Polyphenoloxidase from guava (Psidium guajava L.) by Augustin, M.A., Ghazali, Hasanah M., Hashim, Hasimah

“…Polyphenoloxidase (PPO) was isolated from guava (Psidium guajava L., cv. Kampuchea). Purification by acetone precipitation and dialysis of the Amicon concentrate resulted in 4.9‐fold and 17.2‐fold purification of the dialysed crude extract respectively. …”

Towards a Scalable Proof Engine: A Performant Prototype Rewriting Primitive for Coq by Gross, Jason, Erbsen, Andres, Philipoom, Jade, Agrawal, Rajashree, Chlipala, Adam

“…The resulting extracted command-line compiler is about 1000× faster while featuring simpler compiler-specific proofs. This work lays some foundation for scaling verification efforts and contributes to the broader goal of developing a proof engine with good asymptotic performance, ultimately aimed at enabling the verification of larger and more complex systems.…”
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Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach by Tam, Yew Joon

“…In this, the HBsAg protein plays a vital role as the basic component for anti-HBs detection assays due to its high reactivity and specificity. As such, the present thesis focuses on assessing and optimising key areas related to the downstream processing conditions specifically in the production of HBsAg from recombinant Pichia pastoris and in the establishment of chip-based anti-HBs detection prototype. …”
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Facial spoofing indicator using deep learning by Lim, Eugen Wei Jie

“…Out of all the biometric verification, facial biometric verification is the most common. …”
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Design of a low-voltage low-power WiMAX low noise amplifier by Zeng, Wei

“…CMOS 0.18m CSM technology was used for the proposed design and WiMAX specifications are met satisfactorily.…”
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Experimental and numerical investigations of press-braked stainless steel channel section beam-columns by Zhang, Lulu, Li, Shuai, Tan, Kang Hai, Zhao, Ou

“…In terms of the design consistency, the American specification and Australian/New Zealand standard were shown to result in more consistent resistance predictions (regardless of cross-section dimensions and buckling orientations) than their Eurocode counterpart.…”
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Flexural buckling behaviour and residual strengths of S700 high strength steel circular hollow section columns after exposure to fire by Koh, Alicia

“…The evaluation results generally revealed that the Eurocode yields rather conservative but relatively accurate and consistent buckling resistance predictions, while the American Specification and Australian Standard offer a high level of accuracy and consistency.…”
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Evolutionary origin of the chordate nervous system revealed by amphioxus developmental trajectories by Dai, Y, Zhong, Y, Liu, H, Pan, R, Yuan, L, Du, J, Li, M, Wang, X, Shi, C, Liu, G, Zhu, P, Shimeld, S, Zhou, X, Li, G

“…We characterized cell specification at the mechanistic level and generated mutant lines to examine the function of five key transcription factors involved in neural specification. …”

Age group classification via face images by Lee, Lai Soon.

“…The comparison was based on the accuracy of verifications and the time taken for each simulation. …”
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"Target trial emulation" for observational studies - potential and pitfalls by Hubbard, RA, Gatsonis, CA, Hogan, JW, Hunter, DJ, Normand, S-LT, Troxel, AB

“…To improve the quality of observational studies, target trial emulation requires careful specification of design elements, analytic methods for addressing confounding, and reporting of sensitivity analyses.…”

Production of single-chain variable fragment epidermal growth factor receptor (scFv-EGFR) antibody by Loh, Valerie Kai Li.

“…Results show that the antibody product may not have been produced successfully using the initial expression system due to recurring inconclusive results from purification and characterisation procedures. However, with the use of pSYN1/TG1 expression system and protocol, Western blot results appear to show positive production of scFv-EGFR B10 (synthetic optimized sequence), and specific affinity of the scFv-EGFR B10 antibody product to EGFR is proven.…”
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Structure and subunit arrangement of Mycobacterial F1FO ATP synthase and novel features of the unique mycobacterial subunit δ by Kamariah, Neelagandan, Huber, Roland G., Nartey, Wilson, Bhushan, Shashi, Bond, Peter J., Grüber, Gerhard

“…Expression and purification of the fused mycobacterial b-δ24-446 construct, excluding the membrane-embedded N-terminal amino acids, provided insight into its secondary structure. …”
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Optimised “green solvent” extraction of long-chain menaquinones (Vitamin K2) from wet Lactococcus lactis biomass by Lee, Sze Ying, Hu, Xiao, Stuckey, David C.

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High power amplifier for S-band downlink system of LEO satellites by Tan, Tee Keng.

“…This procedure was followed to determine the specifications for the individual amplifier modules of the HPA. …”
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Machine-learning-assisted design of high strength steel I-section columns by Cheng, Jinpeng, Li, Xuelai, Jiang, Ke, Li, Shuai, Su, Andi, Zhao, Ou

“…Based on the data in the collected database, the regression model trained by Categorical Boosting and existing codified design provisions, as given in the European code and American specification, were assessed and compared. The European code and American specification were found to yield scattered and inaccurate failure load predictions, while the Categorical Boosting trained model led to substantially more accurate and consistent failure load predictions for high strength steel I-section columns with different material grades, boundary conditions, geometric dimensions and failure modes.…”
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HGVS Nomenclature 2024: improvements to community engagement, usability, and computability by Hart, RK, Fokkema, IFAC, DiStefano, M, Hastings, R, Laros, JFJ, Taylor, R, Wagner, AH, den Dunnen, JT

“…The website has been redesigned using modern documentation tools and practices. The specification was updated to include guidance for transcript selection and to align with recent cross-consortia recommendations for the representation of gene fusions. …”

Vaccines: The Health of Nations - A Paradigm Shift by Datta, Shoumen

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Fisher vector faces in the wild by Simonyan, K, Parkhi, O, Vedaldi, A, Zisserman, A

“…Several recent papers on automatic face verification have significantly raised the performance bar by developing novel, specialised representations that outperform standard features such as SIFT for this problem. …”

Use of peptide asparginyl ligases (PALs) in generating alternative non-Ig protein-drug conjugates for cancer therapy by See, Julia Jing Yi

“…Past research on antibody–drug conjugates (ADCs) focused on protein site-specific modification as chemical conjugation lacks site-selectivity. …”
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Enhancing Protein Crystal Nucleation Using In Situ Templating on Bioconjugate-Functionalized Nanoparticles and Machine Learning by McCue, Caroline, Girard, Henri-Louis, Varanasi, Kripa K.

“…Although protein crystallization offers a promising alternative to chromatography for lower-cost protein purification, slow nucleation kinetics and high protein concentration requirements are major barriers for using crystallization as a viable strategy in downstream protein purification. …”
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