Characterizing the interplay between super enhancers and chromatin interactions in cancer by Choudhary, Ruchi

“…Using HiChIP experiments in five human cell lines, we discovered multiple transcription factor (TF) clusters regulating chromatin interactions between SEs/enhancers and promoters, with one hub enriched in c-MYC and YY1. …”
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Investigating the human teashirt genes TSHZ2 and TSHZ3 in pancreatic and endocrine lineage commitment using genome modified human embryonic stem cells (hESC) by Eng, Shermaine Zi Hui

“…The CRISPR RNA-guided FokI-dCas9 nuclease (RFN) genome editing system was employed to create loss-of-function hESC lines. Two guide RNAs revealed successful editing in the 3’ end of TSHZ2 coding sequence while gRNAs tested for the 5’ end of TSHZ2 and for TSHZ3 were ineffective. …”
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Quantum dots-siRNA nanoplexes for gene silencing in central nervous system tumor cells by Lin, Guimiao, Chen, Ting, Zou, Jinyun, Wang, Yucheng, Wang, Xiaomei, Li, Jiefeng, Huang, Qijun, Fu, Zicai, Zhao, Yingying, Lin, Marie Chia-Mi, Xu, Gaixia, Yong, Ken-Tye

“…High gene transfection efficiency (>80%) was achieved on two glioblastoma cell lines, U87 and U251. The gene expression level (49.99 ± 10.23% for U87, 43.28 ± 9.66% for U251) and protein expression level (51.58 ± 7.88% for U87, 50.69 ± 7.59% for U251) of TERT is observed to decrease substantially after transfecting the tumor cells for 48 h. …”
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Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…Compared to the human FKBP12−FK506 complex reported earlier, the structure reveals structural differences in the β5−β6 segment that lines the FK506 binding site. The presence in PfFKBD of Cys-106 and Ser-109 (substituting for His-87 and Ile-90, respectively, in human FKBP12), which are 4−5 Å from the nearest atom of the FK506 compound, suggests possible routes for the rational design of analogues of FK506 with specific antiparasitic activity. …”
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Functional comparison of human Adenomatous Polyposis Coli (APC) and APC-like in targeting beta-catenin for degradation by Schneikert, Jean, Chandra, Shree Harsha Vijaya, Ruppert, Jan Gustav, Ray, Suparna, Wenzel, Eva Maria, Behrens, Jürgen

“…We show here that colon cancer cell lines express also the paralog APC-like (APCL or APC2). …”
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Clonal phylogenies inferred from bulk, single cell, and spatial transcriptomic analysis of epithelial cancers by Erickson, A, Figiel, S, Rajakumar, T, Rao, S, Yin, W, Doultsinos, D, Magnussen, A, Singh, R, Poulose, N, Bryant, RJ, Cussenot, O, Hamdy, FC, Woodcock, D, Mills, IG, Lamb, AD

“…We first performed in-silico comparisons of inferred and directly resolved SNV and CNV status, from single cancer cells, from three different cell lines. We found that inferred SNV phylogenies accurately recapitulate DNA phylogenies (entanglement = 0.097). …”

Performance, heterosis and heritability in selected tropical maize single, double and three-way cross hybrids by Saleh, G.B., Abdullah, D., Anuar, A.R.

“…A series of tropical maize hybrids, involving 10 single, four double and four three-way crosses were evaluated with their parental inbred lines and three control varieties, at Universiti Putra Malaysia, Serdang, Malaysia, in a randomized complete block design, with three replications. …”
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When the Earth Breathes: An Anthology of Volcanic Urbanism by Carucci Alvarez, Maria Gabriela

“…Igneous landscapes, through the production of new basalt floors, rich soils and ocean intrusions, traverse and redefine property boundary lines and national coastlines, which extends beyond plan views and into sectional shifts. …”
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Concept-level sentiment analysis in the manufacturing industry by Teng, He Xu

“…Manufacturers can make use of this technology to improve quality control, standardization and maintenance through producing predictive analyzes of equipment functionality and radically streamlining factory lines. Manufacturing will continue to be an important pillar of Singapore’s economy. …”
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Diameter-free estimates for the quadratic Vinogradov mean value theorem by Mudgal, A

“…Consequently, our strategy differs from these two lines of approach, and we employ techniques from incidence geometry, arithmetic combinatorics and analytic number theory. …”

Ritual speech, performativity, and modality by Frangulov, AM

“…The incorporation of historical data from the ritual corpora of the Rigveda and Hittite ritual tablets, has not only enriched the quality of analysis through quantitative analysis, but it also provided catalysts for many of the discussions raised and many future lines of inquiry. In the examination of the facts of performativity in ritual speech, this thesis has shown that formal semantic consideration of this type of data can reveal many insights into performative speech as a whole. …”

Src family kinase expression and subcellular localization in macrophages: implications for their role in CSF‐1‐induced macrophage migration by Dwyer, AR, Mouchemore, KA, Steer, JH, Sunderland, AJ, Sampaio, N, Greenland, EL, Joyce, DA, Pixley, FJ

“…We report a comprehensive analysis of individual Src family kinase expression in macrophage cell lines and primary macrophages and demonstrate colony‐stimulating factor‐1–induced changes in Src family kinase subcellular localization, which provides clues to their distinct and redundant functions in macrophages. …”

Use of ontology to manage a consumer product website : a study of a mobile phone site by Zhang, Xin

“…Within the ontology, there are five taxonomies to categorize information into a hierarchical structure linking every component with appropriate relations. …”
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Security enhancements to prevent DNS cache poisoning attacks by Chong, Soon Seng

“…One of the main reason was linked to extended loading time for website due to delays and errors caused by signature generation and validation required in DNSSEC, affecting user experience. …”
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Privacy harms by Cofone, IC, Robertson, AZ

“…To do so, we use standard Bayesian statistics to formalize a key intuition: that information privacy is fundamentally linked to how much other people know about you. A key advantage of our model is that, for the first time, it takes privacy preferences seriously while maintaining tractability. …”

Augmented Object Intelligence with XR-Objects by Dogan, Mustafa Doga, Gonzalez, Eric, Ahuja, Karan, Du, Ruofei, Cola?o, Andrea, Lee, Johnny, Gonzalez-Franco, Mar, Kim, David

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Rhetorical contestation and the mediterranean migration crisis, 2013-2018 by He, Wendy Qingli

“…In addition, I argue that the securitization of the migration crisis is perpetuated by the EU and Italy’s use of complicity to construct a political narrative that blurs the lines of responsibility and culpability. By ascribing complicity to others using highly politicized rhetoric, the EU and Italy complicate the narrative of the migration crisis, making it more challenging to attribute responsibility to any one party. …”
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Engineering a surface to enhance endothelial cell adhesion towards creating a 'roll-up' blood vessel prosthesis by Chua, Hui Yee.

“…The optimized gelatin “ink” allowed extended viability for the entrapped HUVECs and the drawn scaffold lines to retain their integrity whilst in growth media. …”
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Impact of BREXIT on key stakeholders of the maritime industry in UK by Ching, Claudia Loke Wei

“…By and large, UK will generally experience detrimental effects in the near future, but the long term effects of BREXIT still awaits to be seen as UK seeks to establish new trade relations and construct necessary infrastructure in supporting the UK economy post-BREXIT. UK shipping lines and port operators are therefore recommended to look to alternative sources of labour to replace the productivity gap of the EU nationals in the worst case scenario of a hard BREXIT should restrictions in the free movement of labour occur after BREXIT. …”
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In vitro and in silico investigation of TRPC3 mechanism and dynamics by Collingridge, J

“…This work provides computational evidence for the ability of TRPC3 to form a widely conserved, mechanistically important π-helix structural component in the pore-lining S6 helix. This has not previously been reported for TRPC3, and simulations presented here suggest a role for the π-helix in promoting a transition towards an open state of the channel. …”