A C-terminal peptide of TFPI-1 facilitates cytosolic delivery of nucleic acid cargo into mammalian cells by Fazil, Mobashar Hussain Urf Turabe, Chalasani, Madhavi Latha Somaraju, Choong, Yeu Khai, Schmidtchen, Artur, Verma, Navin Kumar, Saravanan, Rathi

“…The GGL27 peptide is non-cytotoxic and is internalized in a broad range of mammalian cell-types, including transformed cell lines and primary cells. GGL27 spontaneously forms complexes with nucleic acids of variable sizes, protects them from nuclease degradation, and delivers cargo effectively. …”
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Impact of Using Different Promoters and Matrix Attachment Regions on Recombinant Protein Expression Level and Stability in Stably Transfected CHO Cells by Ho, Steven C. L., Mariati, Yeo, Jessna H. M., Fang, Shiyi Goh, Yang, Yuansheng

“…The information presented here would be valuable to those developing vectors for generation of CHO cell lines with stable and high productivity.…”
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Three-dimensional hydraulic modeling of particle deposition on the patterned isopore membrane in crossflow microfiltration by Choi, Dong-Chan, Jung, Seon-Yeop, Won, Young-June, Jang, Jun Hee, Lee, Jaewoo, Chae, Hee-Ro, Ahn, Kyung Hyun, Lee, Sangho, Park, Pyung-Kyu, Lee, Chung-Hak

“…Particularly, the ratio of crossflow velocity to pore water flux mostly governed the extent of particle depositions (i.e., membrane fouling) on the patterned membrane surface and 3-D modeling based on computational fluid dynamics was also conducted to predict the formation of two distinct stream lines (bulk and vortex) and elucidate the mechanisms of anti-fouling characteristics of patterned membranes.…”
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Multidimensional identification of tissue biomarkers of gastric cancer by Guo, Tiannan, Fan, Lingling, Ng, Wai Har, Zhu, Yi, Ho, Mengfatt, Wan, Wei Keat, Lim, Kiat Hon, Ong, Whee Sze, Lee, Sze Sing, Huang, Shiang, Kon, Oi Lian, Sze, Siu Kwan

“…Recognizing that interrogating plasma membrane proteins individually overlooks extensive interactions among them, we have systematically investigated the membrane proteomes and transcriptomes of six gastric cancer cell lines. Our data revealed aberrantly high expression of proteins whose functions accurately reflect the clinical phenotype of gastric cancer, and prioritized critical RTKs and CD proteins in gastric cancer. …”
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Bacteria-mediated anti-cancer therapy by Bhave, Madhura Satish

“…Subsequent studies were conducted on colorectal cancer cell lines HCT116 (human) and CT26 (murine) to understand the nature of IB- and CM- mediated inhibition. …”
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Generation of lentiviral-mediated shRNA vectors to inhibit the expression of caspase-1 and Pycard/ASC in dendritic cells. by Tan, Bee Kim.

“…Collectively, we have generated an efficient lentiviral vector-mediated shRNA silencing system in primary cell lines which could provide an insight to into the biological mechanisms involved in inflammasome activation.…”
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Analysis of hepatocyte antigen presentation and T cell/hepatocyte interaction in the context of hepatitis B virus Infection. by Noviani, Maria.

“…Nevertheless, as this study was performed with hepatocyte lines, further ex vivo study is needed to validate our current findings. …”
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Discovery of exosomes as gastric cancer biomarkers from conditioned cell culture media. by Low, Su Jun.

“…We isolated exosomes by concentrating 100ml of conditioned cell culture media from 3 gastric cancer cell lines AGS, NCIN87 and IM95, and subjected them to ultracentrifugation. …”
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A study on factors affecting receptivity to traditional chinese medicine In Singapore. by Chu, Hui Wen., Liu, Jia., Tan, Pei Ting.

“…Differences in opinions were also observed along socio-economic lines such as age, education, ethnicity, dominant language and gender. …”
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Effects of hemodynamic shear stress on circulating breast cancer cells by Khairunisa Liyana Abdul Malek

“…In this project, three lines of metastatic breast cancer cells, 231-C3, 231-M1 and 231-M1A were circulated in a microfluidic model mimicking the blood circulatory system over four different time periods: 2 hours, 4 hours, 9 hours and 18 hours while being exposed to three different hemodynamic shear stress at values of 15 dyn/cm2, 30 dyn/cm2 and 45 dyn/cm2. …”
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Laplacian Regularized Subspace Learning for interactive image re-ranking by Zhang, Lining., Wang, Lipo., Lin, Weisi.

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Motive and malice prepensed by Getzler, J

“…I would open the notes arriving from Magdalen with great anticipation, looking forward to discussing the contents with David, and knowing that his lines would set me off into fresh discoveries in libraries and archives for a good few weeks or months. …”

Statistical theory of the broadband two-plasmon decay instability by Rusko, RT, Bingham, R, Silva, LO, Harper, M, Aboushelbaya, R, Myatt, JF, Norreys, PA

“…The instability-quenching properties of a spectrum of discrete lines spread over some bandwidth have also been studied. …”

Paper or Tablet? The Impact of Digital Tools on Sketching During Engineering Design Concept Generation by Das, Madhurima, Huang, May, Yang, Maria

“…This is especially important as tablets can automatically smooth lines and help perfect sketch features, but these features may also take longer to use and may result in fewer sketches produced.…”
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Selective targeting of MYC mRNA by stabilized antisense oligonucleotides by Gill, Taylor, Wang, Haichuan, Bandaru, Raj, Lawlor, Matthew, Lu, Chenyue, Nieman, Linda T, Tao, Junyan, Zhang, Yixian, Anderson, Daniel G, Ting, David T, Chen, Xin, Bradner, James E, Ott, Christopher J

“…MYCASO treatment of cancer cell lines leads to significant inhibition of cellular proliferation while specifically perturbing MYC-driven gene expression signatures. …”
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Unsupervised Learning for Generative Scene Editing and Motion by Fang, David S.

“…In this thesis, we propose two distinct lines of unsupervised learning work with generative applications: 1) BlobGSN and 2) optical flow estimation and flow generation with diffusion models. …”
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Analysis of subtelomeric virulence gene families in Plasmodium falciparum by comparative transcriptional profiling by Witmer, Kathrin, Schmid, Christoph D., Brancucci, Nicolas M. B., Luah, Yen-Hoon, Preiser, Peter Rainer, Bozdech, Zbynek, Voss, Till S.

“…Here, we analysed promoters of upsA, upsB and upsC var, rifA1-type rif, stevor, phist and pfmc-2tm genes and investigated their role in endogenous gene transcription by comparative genome-wide expression profiling of transgenic parasite lines. We find that the three major var promoter types are functionally equal and play an essential role in singular gene choice. …”
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A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system by Shen, Yunbing, Jiang, Long, Iyer, Vaishnavi Srinivasan, Raposo, Bruno, Dubnovitsky, Anatoly, Boddul, Sanjaykumar V., Kasza, Zsolt, Wermeling, Fredrik

“…Here, we present a rapid and universal experimental approach to functionally analyze a CRISPR-targeted cell population that does not involve generating clonal lines. As a simple readout, we leverage the CRISPR-induced genetic heterogeneity and use sequencing to identify how different genotypes are enriched or depleted in relation to the studied cellular behavior or phenotype. …”
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Elucidating the size-dependency of in vitro digested polystyrene microplastics on human intestinal cells health and function by Ma, Yiyuan, Koh, Cheryl Jie Yan, Lim, Hong Kit, Shi, Pujiang, Tay, Chor Yong

“…Herein, the potential deleterious biological effects of pristine and in vitro digested polystyrene (PS) MPs of varying sizes (i.e., 0.1, 1, and 10 µm) are systematically examined over a wide concentration range of 25–400 µg mL−1 on two human intestinal cell lines, namely Caco-2 and NCM 460. Specifically, significant internalization of 0.1 and 1 µm PS -MPs have been observed in both cell types 24 h postexposure. …”
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Label-free assessment of differentiation efficiency in iPSC-derived spinal cord progenitor cells via Magnetic Resonance Relaxometry (MRR) by Tan, Jerome Zu Yao, Chen, Jiahui, Roxby, Daniel, Chooi, Wai Hon, Nguyen, Tan Dai, Ng, Shi-Yan, Chew, Sing Yian, Han, Jongyoon

“…In this study, human iPSCs derived from Cord Lining Endothelial cells were differentiated into Spinal-cord Progenitor Cells (SCPCs) through a 10-day process. …”
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