Investigation of Synthetic Proteins Produced via Automated Fast-Flow Peptide Synthesis by Cowfer, Amanda Elizabeth

“…I will discuss the iterative steps my team took to vary both chemical and mechanical and control variables to improve per-coupling yield enough to enable access to full-length single-domain proteins. On this journey, we utilized test peptides to validate synthesis quality and later synthesized a suite of full-length single-domain biologically active proteins. …”
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Hodge theory and its applications to molecular data analysis by Koh, Ronald Joon Wei

“…Nevertheless, there are currently many methods to call TADs, all of which are not based on a rigorous topological mathematical model. …”
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High-resolution HDX-MS reveals distinct mechanisms of RNA recognition and activation by RIG-I and MDA5 by Liu, Chuan-Fa, Zheng, Jie, Yong, Hui Yee, Panutdaporn, Nantika, Tang, Kai, Luo, Dahai

“…RIG-I and MDA5 are the major intracellular immune receptors that recognize viral RNA species and undergo a series of conformational transitions leading to the activation of the interferon-mediated antiviral response. However, to date, full-length RLRs have resisted crystallographic efforts and a molecular description of their activation pathways remains hypothetical. …”
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Supported Lipid Bilayer Platform To Test Inhibitors of the Membrane Attack Complex: Insights into Biomacromolecular Assembly and Regulation by Yorulmaz, Saziye, Jackman, Joshua Alexander, Hunziker, Walter, Cho, Nam-Joon

“…On the other hand, clusterin directly interacts with C5b-7 such that C5b-7 is still able to bind to the lipid bilayer, and clusterin affects the subsequent binding of other complement proteins involved in the MAC assembly. …”
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Engineering modular platforms for rapid vaccine development by Brune, K

“…Successfully decorating VLPs is still a major challenge. Genetic fusion or chemical modification is often time-consuming and can lead to misassembly or misfolding, which obstructs generation of the desired immune response. …”

Elucidating the mechanism of mRNA transport using novel imaging technologies by Stoychev, D

“…The MS2-MCP system is used to insert into mRNAs of interest special structures to which RNA-binding proteins, fused to a fluorescent protein such as GFP, can then bind. …”

Conformational landscape of the epidermal growth factor receptor kinase reveals a mutant specific allosteric pocket by Kannan, Srinivasaraghavan, Venkatachalam, Gireedhar, Lim, Hong Hwa, Surana, Uttam, Verma, Chandra

“…[Nature, 2016, 534, 129–132] reported a small molecule inhibitor (called EAI045) that binds at an allosteric pocket, does not compete with ATP and displays high potency and selectivity towards certain activating mutants (L858R, T790M, L858R/T790M) of EGFR, with IC50 values ranging from 3 nM to 49 nM. …”
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Amplifying signals in the tumor microenvironment for drug development and diagnostics by Martin Alonso, Maria Carmen

“…At the same time, improving the sensitivity of ctDNA-based tests, that remains limited by the scarcity of ctDNA in blood, holds the key to unlock the full potential of liquid biopsy across many important clinical applications. …”
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Room-temperature continuous-wave vertical-cavity surface-emitting lasers based on 2D layered organic–inorganic hybrid perovskites by Zhang, Hongbo, Hu, Yuzhong, Wen, Wen, Du, Bowen, Wu, Lishu, Chen, Yu, Feng, Shun, Zou, Chenji, Shang, Jingzhi, Fan, Hong Jin, Yu, Ting

“…Two-dimensional (2D) layered lead halide perovskites with large exciton binding energies, efficient radiative recombination, and outstanding environmental stability are regarded as supreme candidates for realizing highly compact and ultralow threshold lasers. …”
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Synthesis, antimalarial activity, and molecular dynamic approaches of salophen metal complexes by Ali, Amatul Hamizah, Mukhtar, Nur Hafizah, Ling, Ng Yee, Norazmi, Nur Aqilah Zahirah, Agustar, Hani Kartini, Ling, Lau Yee, Abd Karim, Nurul Huda, Yusoff, Siti Fairus Mohd, Borzehandani, Mostafa Yousefzadeh, Hassan, Nurul Izzaty

“…Malaria is still one of the main infectious diseases in tropical climates. …”
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Structural and mechanistic insights into the Rel protein of Mycobacterium tuberculosis by Singal, Bharti

“…The NMR spectroscopy titration experiments with branched chain amino acids revealed the specific binding of valine to the MtRel ACT and showed structural rearrangement upon valine binding. …”
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Genotype-specific expression and NLR repertoire contribute to phenotypic resistance diversity in Plantago lanceolata by Safdari, Pezhman, Höckerstedt, Layla, Brosche, Mikael, Salojärvi, Jarkko, Laine, Anna-Liisa

“…Molecular processes contributing to this variation in nature are still poorly known, although theory predicts resistance to evolve at specific loci driven by pathogen-imposed selection. …”
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Nuclei sorting-based genome-wide CRISPR screens to identify novel regulators of hepatic insulin signalling by Martin Perez, E

“…Importantly, the mechanisms underlying hepatic insulin signalling are still not completely understood.</p> <p>This project is focused on identifying genes involved in hepatic insulin action and aims at a more detailed molecular understanding of how defects in the signalling pathway may lead to insulin resistance. …”

π-fused diazocines with controllable photoswitching properties as molecular tweezers by Yuan, Wei, Wu, Mengjiao, Qian, Cheng, Guo, Jingjing, Wu, Yinglong, He, Ting, Wei, Haipeng, Chen, Xiaokai, Wang, Dongdong, Liu, Jiawei, Zhao, Yue, Wang, Shihuai, Yang, Jie, Zhang, Zhongzheng, Chen, Yulan, Zhao, Yanli

“…This work may pave the way for designing novel photoswitches with long half-lives and lightcontrolled properties.…”
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Targeting NAPE-PLD: assessing its potential in the fight against malaria by Goh, Xiu Ling

“…Malaria remains a major global challenge with increasing artemisinin-resistant Plasmodium falciparum. This calls for an increased need for discovering novel ring-stage drug targets. …”
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Synthesis of Au@Pd core/shell nanomaterials for high-performance ethanol oxidation reactions by Xu, Weichang

“…During CV scans, the onset potential and peak current density of backward peak reveals the binding affinity of –OH group and the catalytic activity, respectively. …”
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PESI: paratope-epitope set interaction for SARS-CoV-2 neutralization prediction by Wan, Zhang, Lin, Zhuoyi, Rashid, Shamima, Ng, Shaun Yue Hao, Yin, Rui, Senthilnath, J., Kwoh, Chee Keong

“…Traditional methods rely on features based on first principles derived from the binding interface. However, they are burdened by arduous data preprocessing from a limited quantity of protein structures. …”
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Interpretable Computational Modeling of pre-mRNA Splicing for Multiple Eukaryotic Species by McCue, Kayla M.

“…Together these RNA elements and factors form an intricately interconnected regulatory system which is still incompletely understood. In this thesis, I describe SMsplice, an interpretable computational model of splicing that seeks to improve the understanding of how sequence elements influence the splicing pattern of pre-mRNA transcripts in a variety of eukaryotic organisms. …”
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Boosting hydrogen evolution reaction via electronic coupling of cerium phosphate with molybdenum phosphide nanobelts by Zhang, Luyao, Hu, Minghao, Li, Huan, Cao, Bo, Jing, Peng, Liu, Baocang, Gao, Rui, Zhang, Jun, Liu, Bin

“…Molybdenum phosphide (MoP) is regarded as one of the most promising alternatives to noble-metal based electrocatalysts for efficient hydrogen evolution reaction (HER) due to its similar d-band electronic structure to noble metals and tunable features associated with phase and composition. However, it still remains a great challenge to construct MoP electrocatalysts with abundant active sites that possess ideal H binding strength to promote catalytic performance. …”
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14-3-3 interaction with phosphodiesterase 8A sustains PKA signaling and downregulates the MAPK pathway by Mukherjee, Soumita, Roy, Somesh, Mukherjee, Shruti, Harikishore, Amaravadhi, Bhunia, Anirban, Mandal, Atin K.

“…Conversely, PDE8A activates the MAPK pathway by protecting CRAF/Raf1 kinase from PKA-mediated inhibitory phosphorylation at Ser259 residue, a binding site of scaffold protein 14-3-3. It still remains enigmatic as to how the cross-talk involving PDE8A regulation influences cAMP/PKA and MAPK signaling pathways. …”
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