Use of co-cultivation method to stimulate the production of antimicrobial natural products from mangrove microorganisms by Tan, Jin Wei

“…Cryptic gene clusters from actinomycetes are suspected to harbor potential in producing bioactive products, and in this study, co-cultivation is utilized to stimulate these cryptic gene clusters in novel antibiotics production. 10 actinomycete strains isolated from Sungei Buloh Wetland Reserve were co-cultured with Mycobacterium Abscessus and Asperigillus Westerdijikiae in two liquid mediums, Glucose-Yeast extract-Malt extract (GYM) and Minimum Media (MM). …”
Get full text

Structural basis of RNA binding by leucine zipper GCN4 by Nikolaev, Yaroslav, Pervushin, Konstantin

“…To support further mechanistic studies, herein, we elucidated RNA binding interface of the GCN4 leucine zipper motif from yeast. Solution NMR experiments showed that the LZ-RNA interaction interface is located in the first two heptads of LZ moiety, and that only the dimeric (coiled coil) LZ conformation is capable of binding RNA. …”
Get full text
Get full text

A regulatory role for repeated decoy transcription factor binding sites in target gene expression by Lee, Tek‐Hyung, Maheshri, Narendra

“…Using a synthetic system in budding yeast, we demonstrate that repeated decoy sites inhibit gene expression by sequestering a transcriptional activator and converting the graded dose–response of target promoters to a sharper, sigmoidal‐like response. …”
Get full text

Discovery of stabilized functional mutants of human aquaporin 1 by Soh, Miao Yi

“…A cell-based freeze-thaw functionality assay that was developed by the lab was conducted on yeast Saccharomyces cerevisiae cells transformed with the plasmids to identify the functional mutants, i.e., mutants that express the mutated AQP1 protein that would allow them to exhibit a protective effect in response to rapid freezing. ~ 5% of mutants from a total of 2816 screened from thirty-three 96-well plates were identified after a series of initial and verification screens. …”
Get full text

Optimization of carbon and nitrogen sources for phytase production by Mitsuokella jalaludinii, a new rumen bacterial species by Lan, G.Q., Abdullah, N., Jalaludin, S., Ho, Y.W.

“…Methods and Results: Replacement of glucose, cellobiose and starch in MF1 medium by RB or palm kernel cake and replacement of trypticase peptone and yeast extract in the medium by SM or enzymatic digested soybean milk significantly increased the phytase production by M. jalaludinii. …”
Get full text

The Malaysian 'Islamic' state versus the Islamic state (IS) : evolving definitions of 'Terror' in an 'Islamising' nation-state by Chan, Nicholas Wai Yeap

“…Akin to a case of semantic acrobatics, we demonstrate how the Malaysian state securitised (1980-2001), normalised (2001-2013), and resecuritised (2014 onwards) the term as a result of a balancing act which not only aimed at neutralising the elements of militancy, but also to augment the state's ' Islamic ' credentials in a bid to outplay its major Islamist opponent, the Pan Malaysian Islamic Party (PAS). At the end, following the emergence of a transnational insurgent/quasi-state/ terrorist/ideology hybrid, the Islamic State (IS), we argue that the Malaysian state is now embroiled in an ' Islamic state versus Islamic State' trap, where the state after appropriating and normalising the ' Islamic state' ideal, has no choice but to contend with what is fundamentally a ' terrorist' group (by the state's own definition) in religious terms. …”
Get full text

Effects of fermented products on performance, faecal pH, Enterobacteriaceae and lactic acid bacteria counts and interrelationships, and plasma cholesterol concentration in rats by Loh, T., Foo, H., Tan, S., Goh, Y., Shukriyah, M., Kufli, C.

Get full text

Electrical characterization of bias temperature instability in MOSFETs with the ultrathin SiON and la-doped HfSiO gate dielectrics by Du, Guoan.

“…The initial stage of the project involved the development of a new ultra-fast switching (UFS) method for the measurement of the bias temperature instability (BTI) phenomenon in state-of-the-art MOSFETs. …”
Get full text

Phase separation of a microtubule plus-end tracking protein into a fluid fractal network by Czub, MP, Uliana, F, Grubić, T, Padeste, C, Rosowski, KA, Lorenz, C, Dufresne, ER, Menzel, A, Vakonakis, I, Gasser, U, Steinmetz, MO

“…Here, we have study the phase separation of Bik1, a CLIP-170 family member and key +TIP involved in budding yeast cell division. Bik1 is a dimer with a rod-shaped conformation primarily defined by its central coiled-coil domain. …”

Performance evaluation of SAFIS for benchmark problems by Sinara Vijayan.

“…Based on these studies, an improved SAFIS algorithm called Fast SAFIS is developed. The optimal parameters of Fast SAFIS algorithm are determined and a performance evaluation of Fast SAFIS is done with SAFIS and other algorithms. …”
Get full text

Modelling pH dynamics, SCOBY biomass formation, and acetic acid production of Kombucha fermentation using black, green, and Oolong Teas by Chong, Ann Qi, Chin, Nyuk Ling, Talib, Rosnita A., Basha, Roseliza Kadir

“…Kombucha is a traditional, fermented beverage made with an essential biomaterial known as SCOBY (symbiotic culture of bacteria and yeast). Three different tea types, namely black, green, and oolong, were compared in kombucha fermentation in terms of pH dynamics, the formation of SCOBY biomass, and the production of acetic acid. …”
Get full text

Application of scoby bacterial cellulose as hydrocolloids on physicochemical, textural and sensory characteristics of mango jam by Chong, Ann Qi, Chin, Nyuk Ling, A. Talib, Rosnita, Kadir Basha, Roseliza

“…BACKGROUND: The scoby pellicle of symbiotic culture of bacteria and yeast is a by-product from kombucha fermentation. …”

Performance evaluation of TCP/IP traffic over ABR service by Maung Hla Min.

“…Then we will go into the TCP congestion control mechanisms, which in specific, include slow start, congestion avoidance, fast retransmit and fast recovery. We will discuss the advantages and disadvantages of using TCP Tahoe, TCP Reno and TCP Vegas congestion control mechanisms. …”
Get full text

A low-cost, open-source cylindrical Couette rheometer by Erni, Makita, Hart, A. John, Trumper, David, Owens, Crystal E.

“…To establish the measurement range, the viscosity of four Newtonian samples of 0.1–10 Pa.s were measured with the OSR and compared to benchmark values from a laboratory rheometer, showing under 23% error. …”
Get full text

Prenatal auditory stimulation and impacts on physiological response to feed restriction in broiler chickens at market age by Ahmad-Hanafi, S., Zulkifli, I., Ramiah, S.K., Chung, E.L.T., Kamil, R., Sazili, A.Q., J., Mashitah

“…On d 42, an equal number of birds from each prenatal auditory stimulation (PAS) group were subjected to either ad libitum feeding (AL) or 30-h of feed restriction (FR) in a completely randomised design. …”
Get full text

Development of fed-batch strategy to produce bacteria cellulose by Chua, Gek Kee, Soo, Yang Kee

“…First, batch fermentation mode was carried out to determine the glucose consumption profile of Gluconacetobacter xylinus using Yeast Extract Glucose Chloramphenicol Medium (YGC). …”
Get full text

Molecular characterization of Candida auris outbreak isolates in Qatar from patients with COVID-19 reveals the emergence of isolates resistant to three classes of antifungal drugs by Abid, Fatma Ben, Salah, Husam, Sundararaju, Sathyavathi, Dalil, Lamya, Abdelwahab, Ayman H., Salameh, Sarah, Ibrahim, Emad B., Almaslmani, Muna A., Tang, Patrick, Perez-Lopez, Andres, Tsui, Clement Kin-Ming

“…During the COVID-19 pandemic in Qatar, many patients who were severely ill were colonized and infected by Candida auris, an invasive multidrug-resistant yeast pathogen that spreads through nosocomial transmission within healthcare facilities. …”
Get full text

Legionella pneumophila filamentous morphological form and the generation of novel spherical form upon interaction with protozoan hosts by Chan, Sock Hoai

“…Our investigation showed that filamentation cannot be neatly pegged to a single dominant factor but is promoted under a concerted influence of environmental conditions leaning towards (i) higher than ambient temperatures, at 37-42°C, (ii) static fluid movement, and (iii) with at least a minimal organic content of 5% buffered yeast extract, or equivalent. In the course of investigating interaction of the filamentous form with protozoan hosts, a spherical form was discovered. …”
Get full text

Characterization of the near native conformational states of the SAM domain of Ste11 protein by NMR spectroscopy by Gupta, Sebanti, Bhattacharjya, Surajit

“…SAM domain of the Ste11 protein of budding yeast plays important roles in mitogen-activated protein kinase cascades. …”
Get full text
Get full text

On the total synthesis of complex alkaloids by Shennan, BDA

“…Following a concerted investigation into C−H oxidation for the former and a transfer of chirality approach for the latter, a Baker’s yeast reduction is adopted as an efficient route towards an enantioenriched, oxidised pyrrolidine building block which is advanced to a pentacyclic intermediate.…”