The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice by Carvalho, P, Gomes, C, Gonçalves, I, Lourenço, TF, Vlad, D, Langdale, JA, Saibo, NJM

“…To engineer C₄ into a C₃ plant, it is essential to understand how C₄ genes, such as <i>phosphoenolpyruvate carboxylase (PEPC1)</i>, are regulated to be expressed at high levels and in a cell-specific manner.</li> <li>Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the <i>Setaria viridis PEPC1</i> promoter. …”

Recent insight into the advances and prospects of microbial lipases and their potential applications in industry by Eskandari, Azadeh, Thean, Chor Leow, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…This updated review presents new knowledge about various origins of microbial lipases, such as fungi, bacteria, and yeast. It highlights both the traditional and modern purification methods, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, the aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF), moreover, delves into the diverse applications of microbial lipases across several industries, such as food, vitamin esters, textile, detergent, biodiesel, and bioremediation. …”

On zero-dimensional polynomial systems and their applications to the elliptic curve discrete logarithm problem by Yun, Yang

“…We give an upper bound of the last fall degree of the Weil descent system of $\mathcal{F}$ from $k$ to $k'$, which depends on $q$, $m$, the last fall degree of $\mathcal{F}$, the degree of $\mathcal{F}$ and the number of solutions of $\mathcal{F}$, but not on $n$. …”
Get full text

Design of system for parking and repositioning of bikes in a public bike-sharing system by Siong, Jun Wei

“…Next, the concept of the Wagner-Whitin model is used to determine the bike reallocation plan that incurs the least cost. Lastly, the proposed model accounts for the returning bikes, which is important as it will affect the inventory and thus, inventory cost, from one period to another. …”
Get full text

Behaviour of reinforced welded tubular joints by Wong, Looi Kian.

“…In the offshore industry, a common operation problem is the last minute change of barge scheduled for transporting an offshore jacket to the designated location for installation while fabrication of the jacket is almost complete. …”
Get full text

Unexpected anthropogenic emission decreases explain recent atmospheric mercury concentration declines by Feinberg, Aryeh, Selin, Noelle E., Braban, Christine F., Chang, Kai-Lan, Custódio, Danilo, Jaffe, Daniel A., Kyllönen, Katriina, Landis, Matthew S., Leeson, Sarah R., Luke, Winston, Molepo, Koketso M., Murovec, Marijana, Nerentorp Mastromonaco, Michelle G., Aspmo Pfaffhuber, Katrine, Rüdiger, Julian, Sheu, Guey-Rong, St. Louis, Vincent L.

“…Global anthropogenic emission inventories report increases in Northern Hemispheric (NH) Hg emissions during the last three decades, in contradiction with the observed decline in atmospheric Hg concentrations at NH measurement stations. …”
Get full text

Impact resistance of reinforced concrete members by Low, Kun Sheng

“…As building structures became modernized, there is an increased need to understand the behaviour of concrete structures against extreme loading conditions such as impact, blast and earthquakes. In this paper, we will focus on the impact resistance of reinforced concrete members. …”
Get full text

Heat of hydration, bleeding, viscosity, setting of Ca(OH)<inf>2</inf>-GGBS and MgO-GGBS grouts by Yu, Hua, Yi, Yaolin, Unluer, Cise

“…Hydrated lime (Ca(OH)2)- and reactive magnesia (MgO)-activated ground granulated blast-furnace slag (GGBS) have shown advantages in geotechnical applications compared with Portland cement (PC). …”
Get full text

Quantifying the internal and external drivers of Southeast Asian rainfall extremes on decadal timescales by Wang, Shouyi, Ummenhofer, Caroline C., Murty, Sujata A., Nguyen, Hung T. T., Buckley, Brendan M.

“…Historical records and paleoclimate archives that span the last millennium reveal extreme multi-year rainfall variations that significantly affected the societies of mainland Southeast Asia. …”
Get full text

Drawing memories. by Chang, Jerly Wei Kiang.

“…The film tells a simple and heart wrenching story about a little boy who is brought back to the last reminiscence of his mother. It illustrates a little boy's pain and sorrow of not being able to let go of the death of his mother and how he eventually was able to pick up the courage to move on after given the opportunity to see his mother for the very last time. …”
Get full text

A brief on Saudi Arabia’s Energy Efficiency Program (SEEP) by Bin Salman Al-Saud, A

“…The Kingdom has witnessed unprecedented economic and industrial development in the last decades, which has led to an increase in the Kingdom’s domestic energy consumption. …”

A study of GPS based attitude determination technique by Dai, Lin

Get full text

CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome by Dalvie, Neil C., Lorgeree, Timothy R., Yang, Yuchen, Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J., Del Rosario, Amanda M., Love, Kerry R., Love, J. C.

“…Background The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. …”
Get full text

Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site by Banach, Bailey B, Pletnev, Sergei, Olia, Adam S, Xu, Kai, Zhang, Baoshan, Rawi, Reda, Bylund, Tatsiana, Doria-Rose, Nicole A, Nguyen, Thuy Duong, Fahad, Ahmed S, Lee, Myungjin, Lin, Bob C, Liu, Tracy, Louder, Mark K, Madan, Bharat, McKee, Krisha, O’Dell, Sijy, Sastry, Mallika, Schön, Arne, Bui, Natalie, Shen, Chen-Hsiang, Wolfe, Jacy R, Chuang, Gwo-Yu, Mascola, John R, Kwong, Peter D, DeKosky, Brandon J

“…Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. …”
Get full text

Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. …”
Get full text
Get full text

Finding transcription factor binding motifs for coregulated genes by combining sequence overrepresentation with cross-species conservation by Jia, Hui., Li, Jinming.

“…Compared with the prevailing motif finding software, our method has some advantages in finding transcriptional factor binding motifs for potential coregulated genes if the gene upstream sequences of multiple closely related species are available. Although we used yeast genomes to assess our method in this study, it might also be applied to other organisms if suitable related species are available and the upstream sequences of coregulated genes can be obtained for the multiple closely related species.…”
Get full text
Get full text

Niemann-Pick type c disease: pathogenesis and therapy by Colaco, A

“…To gain further insight into the function of NPC1 and proteins it interacts with we used the yeast orthologue, Ncr1p. I recreated the Δncr1 mutant and characterized the mutant using an array of systematic screens to identify different processes and pathways that may play a role in NPC pathogenesis. …”

Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods by Mohd Yusof, Rokiah, Haque, Formuzul, Ismail, Maznah, Hassan, Zaiton

“…Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. …”

Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants by Eskandari, Azadeh, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. …”

Prioritising conservation areas in Southeast Asia: highlighting zoological biodiversity hotspots and assessing spatiotemporal patterns of the felids guild by Chiaverini, L

“…Specifically, biodiversity hotspots have originally been identified as those global areas containing at least 0.5% or 1,500 of the World’s vascular plant species as endemic and that have lost at least 70% of their primary vegetation. …”