Biased competition between Lgr5 intestinal stem cells driven by oncogenic mutation induces clonal expansion by Snippert, Hugo J., Schepers, Arnout G., van Es, Johan H., Simons, Benjamin D., Clevers, Hans

“…Thus, an unequal competition between wild‐type and mutant intestinal stem cells initiates a biased drift that leads to the clonal expansion of crypts carrying oncogenic mutations.…”
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Functional characterization of orexin system by Chua, Peiling

“…Also, currently there is an absence of a good behavioral paradigm which mimics major depression so we hope to develop one by applying chronic stress through forced swimming test, together with clomipramine, to male wild-type mice. However, the results were not on par with our assumptions as we presumed that the mice will develop depression-like symptoms after 7 days of treatment and we also did not manage to generate the viral vectors. …”
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Improving gene therapy by Wang, Eliza Lisha

“…Retroviruses produced from these packaging lines transfected with the recombinant envelope plasmids were used to infect wild-type Jurkat T-cells. FACS analysis of these Jurkat T-cells indicated that the packaging lines did produce retroviral particles expressing the env protein. …”
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The role of PTEN in human bone marrow-derived mesenchymal stem cells mediated tumor cells suppression. by Toh, Xin Yi.

“…In parallel, isogenic glioma cell lines U251, which overexpresses wild-type PTEN upon tetracycline treatment, resulted in 1.43-fold glioma cell growth retardation when cultured in the presence of MSC. …”
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Characterization of the alternative splicing regulating the proapoptotic gene BIM. by Lee, Qing Feng.

“…Eight-nucleotide serial deletions were introduced to a chimeric BIM minigene to investigate on the presence of cis-acting elements in BIM Exon 4. All wild-type and mutated BIM minigenes resulted in 100% inclusion of Exon 4. …”
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Translation termination mechanism studied by solution state NMR by Li, Yan

“…Through comparison of the solution structure of Q122FM(Y)F126 mutant, the Y125F mutant of eRF1 N-domain and the wild type eRF1 N-domain which are determined, we built the correlation between the structure and the stop-codon recognition and found that the conserved GTS loop adopts alternate conformations. …”
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The aging effects of serial passaging in neural progenitor cells for in vitro neurodegenerative disease modelling by Low, Selwyn Jun Xi

“…In this study, the effects of serial passaging on the proliferation and differentiation capabilities of wild type (WT) and Parkinson's disease (PD) NPCs were evaluated by assessing gene expression changes across early and late passaged cells. …”
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Preliminary study on the effect of high and low cyclic-di-GMP level on 3-chloroaniline biodegradation by Comamonas testosteroni by Qian, Duoduo

“…Comamonas testosteroni WDL7 wild type, yedQ, and yhjH mutants were incubated with 3-cholroanilne as the sole carbon source in planktonic environment, in which mutant yedQ showed a better performance on growth and surviving, pyhjH mutant showed a delayed degradation profile.…”
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"Kemunting : a journey" - Malay identity in pre- and post- independence Singapore by Muhammad Hidhir Md Razak

“…The Malay name for the rose myrtle, "kemunting" refers to the shrubs commonly found growing wild along the sandy stretches of the seaside where Yah spends most of her days. …”
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Viral determinants of enterovirus 71 neuroinvasive potential by Rachel

“…A clinical isolate of EV71 strain 41 wild type (WT) together with the infectious clones were used to determine the relative expression of EV71 in human rhabdomyosarcoma (RD) cells. …”
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Bacteria encode post-mortem protein catabolism that enables altruistic nutrient recycling by Gibson, SER, Frost, I, Hierons, SJ, Moses, T, Poon, WCK, West, SA, Cann, MJ

“…Growth assays reveal that the lysate of Lon protease-null bacteria does not provide a growth benefit to wild type cells. This deficiency is reversed with exogenous recombinant Lon protease, confirming its post-mortem role and is independent of Lon ATPase activity. …”

Mechanical problem solving by plush-crested jays: are tools special after all? by Lois-Milevicich, J, Rat-Fischer, L, de la Colina, MA, Gómez, RO, Reboreda, JC, Kacelnik, A

“…We investigate this problem using plush-crested jays (Cyanocorax chrysops), a corvid new to cognitive research that shows highly flexible and inquisitive behaviour in the wild and has not been reported to use tools. We tested jays in two tasks of apparent similar manipulative complexity and incentive, one involving a tool (T) and the other not (NT). …”

TagAlong: Informal Learning from a Remote Companion with Mobile Perspective Sharing by Greenwald, Scott W., Khan, Mina, Vazquez, Christian D., Maes, Pattie

“…It was developed to work robustly in the wild, depending only on widely-available components and infrastructure. …”
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Use of genetically modified bacteria to repair cracks in concrete by Zhang, Zhigang, Weng, Yiwei, Ding, Yuanzhao, Qian, Shunzhi

“…The results show that the crack-repair efficiency was enhanced very evidently by spraying genetically modified bacterial-liquid as no microbiologically induced calcite precipitation (MICP) was found within the cracks for concrete samples sprayed using wild type bacterial-liquid. In addition, the crack-repair process was also shortened significantly in the case of genetically modified bacteria.…”
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Sorptive properties of biofilm matrix for heavy metal sequestering by Elisa Tiaras

“…The characteristics and the ability to treat heavy metals in 4 strains, namely PAO1 wild-type, ΔPelA strain, ΔPslBCD strain and double mutant ΔPelA- ΔPslBCD strain, were compared in the study. 2 types of metals that are commonly found in the environment were used in the study: copper (Cu) and zinc (Zn). …”
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Computational investigations of HIV-1 protease and protease inhibitors interactions by Sham, Hoi Fung

“…This was done by using molecular docking of PIs with HIV-1 proteases using AutoDock Vina to predict the binding conformations of the respective docked complexes. Both wild type HIV-1 proteases and mutated HIV-1 proteases were used in the docking simulations. …”
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The role of EZH2-VAV interaction in tumorigenesis by Wong, Jong Fu

“…While cytosolic EZH2 shows greater transforming and metastatic capacity than wild-type EZH2, targeted disruption of EZH2-VAV interaction abolishes EZH2-promoted tumorigenesis. …”
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Physiological functions of fungus-derived prostaglandin E₂ in Candida albicans by Leong, Royston Weng Onn

“…Neither PGE2 supplementation nor blockade altered the in vitro growth or virulence of C. albicans, and an ole2/ole2 fungal mutant defective in PGE2 production manifested similar virulence as the wild-type strain in a mouse model of disseminated candidiasis. …”
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Consensus on current management of endometriosis by Johnson, NP, Hummelshoj, L, World Endometriosis Society Montpellier Consortium

Climate change: crop performance and potential by Ramlan, Mohd Fauzi

“…A similar study has been done in the production of bell pepper in lowland areas. In-depth analyses on plant partitioning and yield components are highlighted. …”
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