Intraperitoneal injection of the Porphyromonas gingivalis outer membrane vesicle (OMV) stimulated expressions of neuroinflammatory markers and histopathological changes in the brai... by Adewoyin, Malik, Hamarsha, Ahmed, Akinsola, Rasaq, Teoh, Seong Lin, Azmai, Mohammad Noor Amal, Abu Bakar, Noraini, Nasruddin, Nurrul Shaqinah

“…Brains of experimental zebrafish were dissected at desired time points for colorimetric assays, ELISA, and histology. This study discovered that nitric oxide and PGE2 were significantly increased at 45 min, while IL-1β and IL-6 were expressed at subsequent 12 h and 24 h time points, respectively. …”
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Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma by Lyu, Zhenlei, Mahenderan, Appukutty, Radhakrishnan, Ammu Kutty G. K., Chin, Yit Siew, Yin, Chao

“…CCK-8 and colony formation assays were used to detect cell viability and proliferation. ELISA was employed to determine the levels of cytokines secreted by macrophages. …”
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Assessment of phagocytosis and cytokine secretions by monocytes in the presence of plasmodium falciparum by Xuan, Keh Min

“…falciparum, to assess phagocytic capability of infected red blood cells by human monocytes and further measure the cytokine secretions of monocytes following phagocytosis by using ELISA. In this study, monocytes were isolated from whole blood collected from healthy individuals while Plasmodium falciparum (3D7) was cultured under optimal conditions. …”
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Effects of transport inhibitors on the cellular uptake of liposomes from mycobacterium smegmatis by human monocytes dendritic cells by Nordin, Nor Asyikin

“…Supernatant will be collected to measure the cytokine level by ELISA. Therefore, in this proposed study, we are going to fill up the gaps by investigating the uptake mechanisms of liposomes-Msmeg as a potent lipid-based vaccine by DCs from PBMC in healthy individuals.…”
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Essays on the dynamics of the U.S. real exchange rate by Li, Yingzhu

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Towards a Single Bio-molecule Detector Based on CMOS Nanofluidic Platform by Zikrallah, Ahmed S.

“…Ideally, we would be able to continuously monitor the secretome of these cell therapies as they are transformed and expanded in manufacturing.However, state-of-theart techniques for monitoring typically low concentrations of cytokines require either Mass Spectroscopy (MS) or immunoassays like Enzyme-linked Immunosorbent Assay (ELISA). We propose the use of CMOS technology to build a proteomic platform with a single biomolecule resolution. …”
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Skin dendritic cell and T cell activation associated with dengue shock syndrome by Duyen, Huynh Thi Le, Cerny, Daniela, Trung, Dinh The, Pang, Jassia, Velumani, Sumathy, Toh, Ying Xiu, Qui, Phan Tu, Hao, Nguyen Van, Simmons, Cameron, Haniffa, Muzlifah, Wills, Bridget, Fink, Katja

“…We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. …”
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The chronic cerebral hypoperfusion model induces proinflammatory cascades in Alzheimer's disease by Abedi, Zahra, Basri, Hamidon, Hassan, Zurina, Inche Mat, Liyana Najwa, Khaza’ai, Huzwah, Mohd Ali, Razana

“…The gene expression levels of cathepsin B, NLRP3, an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 were analysed using real-time PCR, while the expression levels of the inflammatory cytokines were estimated using the ELISA method. Structural damage to the hippocampus was assessed using hematoxylin and eosin (HE) staining. …”
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Micro electro mechanical systems (MEMS) enabled digital pen by Tan, Joel Qi Zhi

“…Upon completing the data collection, data were imported into AlaVar5 to compute the Allan standard deviation of each axis of the accelerometer and gyroscope. …”
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Nuclear magnetic resonance spectroscopic studies of the structure and interactions between hepatitis B virus core and surface antigens with peptides by Muhamad, Azira

“…In a previous study, a disulfide constrained cyclic peptide cyclo S1,S9 Cys-Glu-Thr-Gly-Ala-Lys-Pro-His-Cys (S1, S9-cyclo-CETGAKPHC) was isolated from a phage displayed cyclic peptide library using an affinity selection method against HBsAg. …”
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Seroepidemiology of COVID-19 in pregnant women and their infants in Uganda and Malawi across multiple waves 2020-2022 by Hookham, L, Cantrell, L, Cose, S, Freyne, B, Gadama, L, Imede, E, Kawaza, K, Lissauer, S, Musoke, P, Nankabirwa, V, Sekikubo, M, Sommerfelt, H, Voysey, M, PeriCOVID Consortium, Le Doare, K

“…A nasopharyngeal swab was taken from mothers at enrolment for RT-PCR confirmation of SARS-CoV-2 infection, and maternal and cord blood samples were tested for SARS-CoV-2 antibodies using Wantai and Euroimmune ELISA. The primary outcome was seroprevalence of SARS-CoV-2 antibodies in maternal blood, reported as the proportion of seropositive women by study site and wave of COVID-19 within each country. …”

Effects of ifenprodil treatment on pain behaviour, inflammation, and nociceptive responses in the spinal cord of complete freund’s adjuvant-induced chronic polyarthritis rat by Noh, Ain’ Sabreena Mohd

“…Histopathological examination was performed on the ipsilateral ankle joint while the lumbar region of the spinal cord (L4-L5) was collected for ELISA, immunohistochemistry and RT-qPCR analyses. …”
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Cytotoxicity assessment of α helix Antarctic yeast oriented antifreeze peptide (Afp1m) on M. dunni (Clone III8C) cells by Khan, Muhammad Shuaib, Abdul Rahman, Mohd Basyaruddin, Abu Bakar, Mohd Zuki, Noordin, Mohammed Mustapha, Ullah, Shakeeb, Abdul Abubakar, Adamu, Rehman, Saifur, Saddiqua, Aisha, Mohammad Yusof, Loqman

“…The findings of this work indicate that Afp1m exhibits cryoprotective properties without inducing toxicity when used for the cryopreservation of M. dunni (Clone III8C) cells. were cul t ured wi t h 10% FBS (Foet al Bovine Serum) using an Elisa reader and in medium containing various amounts (10, 5, 2, 1 and 0.5 mg/mL) of Afp1m, the control group (10% FBS) displayed varying survival percentages (78.86 ± 10.17%, 88.38 ± 3.19%, 88.75 ± 7.19 %, 90.61 ± 7.11%, 91.19 ± 4.52%, and 100.00 ± 0.0 %) throughout 24 hr. …”
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3D printed magnetic digital microfluidics for biomedical applications by Kanitthamniyom, Pojchanun

“…Here, the detection of hepatitis B surface antigen using enzyme-linked immunosorbent assay (ELISA) was demonstrated on the new platform for the potential diagnostics of hepatitis. …”
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Leucine-rich α-2-glycoprotein 1 suppresses endothelial cell activation through ADAM10-mediated shedding of TNF-α receptor by Pang, Kuin Tian, Ghim, Mean, Liu, Chenghao, Tay, Hui Min, Fhu, Chee Wai, Chia, Rui Ning, Qiu, Beiying, Sarathchandra, Padmini, Chester, Adrian H., Yacoub, Magdi H., Wilkinson, Fiona L., Weston, Ria, Warboys, Christina M., Hou, Han Wei, Weinberg, Peter D., Wang, Xiaomeng

“…LRG1 in endothelial cells (ECs) of arteries and serum of patients with critical limb ischemia (CLI) was assessed by immunohistochemistry and ELISA, respectively. LRG1 expression in sheared and tumor necrosis factor-α (TNF-α)-treated ECs was analyzed. …”
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Magnetic nanochain enabled microarray and microfluidic bioassays by Xiong, Qirong

“…The magnetic dynamic nanomixers were expected to find widespread uses in on-surface bioassays that suffer from diffusion limitation, such as microfluidic assays, enzyme-linked immunosorbent assay (ELISA), and heterogeneous catalysis. Finally, we used this dual-functional magnetic nanochains in microfluidic bioassay. …”
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Development of human enterovirus 71 nano calcium phosphate adjuvanted candidate vaccine for parenteral and mucosal delivery by Saeed Ahmed, Mohamed Ibrahim

“…The samples were examined for the presence of HEV71 specific IgG and IgA antibody classes in the serum and saliva using an in-house developed ELISA assay. The in vitro delivery of adjuvant loaded polymeric carrier offered an improved and sustained release of the nano-adjuvant encapsulated in chitosan but not alginate, due to the reversibility of adjuvant-polymer interaction. …”
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Evaluation of diabetic wound healing properties of moringa oleifera lam using in vitro and in vivo wound models by Muhammad Amali, Abubakar

“…Proinflammatory cytokines analyses were performed using ELISA, Western blotting and immunohistochemistry techniques. …”
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Semiconductor quantum well photodetector for fibre optical communication application by Li, Dao Sheng

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Paradoxical reaction to antitubercular treatment in a case of pulmonary tuberculosis by Paudyal, B, Paudel, K, Shakya, M, Basnyat, B