CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome by Dalvie, Neil C., Lorgeree, Timothy R., Yang, Yuchen, Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J., Del Rosario, Amanda M., Love, Kerry R., Love, J. C.

“…Background The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. …”
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Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site by Banach, Bailey B, Pletnev, Sergei, Olia, Adam S, Xu, Kai, Zhang, Baoshan, Rawi, Reda, Bylund, Tatsiana, Doria-Rose, Nicole A, Nguyen, Thuy Duong, Fahad, Ahmed S, Lee, Myungjin, Lin, Bob C, Liu, Tracy, Louder, Mark K, Madan, Bharat, McKee, Krisha, O’Dell, Sijy, Sastry, Mallika, Schön, Arne, Bui, Natalie, Shen, Chen-Hsiang, Wolfe, Jacy R, Chuang, Gwo-Yu, Mascola, John R, Kwong, Peter D, DeKosky, Brandon J

“…Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. …”
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Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. …”
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Finding transcription factor binding motifs for coregulated genes by combining sequence overrepresentation with cross-species conservation by Jia, Hui., Li, Jinming.

“…Compared with the prevailing motif finding software, our method has some advantages in finding transcriptional factor binding motifs for potential coregulated genes if the gene upstream sequences of multiple closely related species are available. Although we used yeast genomes to assess our method in this study, it might also be applied to other organisms if suitable related species are available and the upstream sequences of coregulated genes can be obtained for the multiple closely related species.…”
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Niemann-Pick type c disease: pathogenesis and therapy by Colaco, A

“…To gain further insight into the function of NPC1 and proteins it interacts with we used the yeast orthologue, Ncr1p. I recreated the Δncr1 mutant and characterized the mutant using an array of systematic screens to identify different processes and pathways that may play a role in NPC pathogenesis. …”

Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods by Mohd Yusof, Rokiah, Haque, Formuzul, Ismail, Maznah, Hassan, Zaiton

“…Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. …”

Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants by Eskandari, Azadeh, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. …”

UMPSA hosts RBTX Petrosains Challenge Eastern Zone 2024 by Naqiah, Puaad

“…PEKAN, 4 September 2024 - Universiti Malaysia Pahang Al-Sultan Abdullah (UMPSA) hosted the RBTX Petrosains Challenge East Zone 2024 Competition at the Multipurpose Hall, UMPSA Pekan.…”
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International market entry. by Lim, Ley Yen., Soh, Stephanie Yan Ling., Tan, Yee Fei.

“…This Applied Research Project attempts to provide an insight on international expansion by Singapore businesses into South East Asia, specifically in the food service industry. …”
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One Plus One is More than Two: A Practical Combination of Power and Fault Analysis Attacks on PRESENT and PRESENT-Like Block Ciphers by Patranabis, Sikhar, Mukhopadhyay, Debdeep, Breier, Jakub, Bhasin, Shivam

“…We present the first practically realizable sidechannel assisted fault attack on PRESENT, that can retrieve the last round key efficiently using single nibble faults. …”
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THE MAYFLY / by Hazel, James, author 656313

“…When lawyer Charlie Priest is attacked in his own home by a man searching for information he claims Priest has, he is drawn into a web of corruption that has its roots in the last desperate days of World War Two. When his attacker is found murdered the next day, Priest becomes a suspect and the only way to clear his name is to find out about the mysterious House of Mayfly - a secret society that people will kill for. …”

Selective load shedding priority algorithm development by Ong, Jing Xian

“…In modern power systems, load shedding is usually the last resort to maintain system stability. Raising the power generated is the go-to method to tackle increasing load demand. …”
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Nonlinear partial differential equations in neuroscience: from modelling to mathematical theory by Carrillo de la Plata, JA, Roux, P

“…The second part concerns hyperbolic transport equations, namely the model of the Time Elapsed since the last discharge and the jump-based Leaky Integrate and Fire model. …”

Enhanced monsoon-driven upwelling in Southeast Asia during the Little Ice Age by Chen, Mengli, Martin, Patrick, Ren, Haojia, Zhang, Run, Samanta, Dhrubajyoti, Chen, Yi-Chi, Hughen, Konrad A., Phan, Kim Hoang, Vo, Si Tuan, Goodkin, Nathalie Fairbank

“…Our data indicates a link between surface ocean nutrient supply and the East Asian Summer Monsoon strength in an economically important marginal sea. …”
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Empowering the employability of people with disability (PWD) through a skills intervention program by Omar, Muhd Khaizer, Mohd Puad, Mohd Hazwan, Yaakub, Mohamad, Muslim, Marlinah, Gui, Qing

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Empirical examination of economic linkages between Japan and rest of Asia. by Lim, Kok Soon., Yong, Kin Loong.

“…The overall objective of our paper is to use econometric analysis to empirically examine whether the Japanese economy does, in fact, have strong effects on other East Asian economies. Our results indicate that although Japanese economic performance does affect the performance of the region’s economies, the effects are weaker than might be expected. …”
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Xi's 'balance of interest solution': pre-empting an Asian NATO? by Suryanarayana, P. S.

“…He seems to suggest that the balance of national interests, not balance of power, is better at promoting a regional order for Asia or East Asia too.…”
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New York Stock Exchange (NYSE) : Day-of-the-week effect and bid-ask spreads by Guo, Lisa Xiuhui, Ernest Julian Sutanto, Quek, Benny Shiwei

“…We find that the technology sector is associated with higher transaction cost. Last but not least, our results in the last section present the absence of DOW effect in all three types of classifications by trading volume.…”
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The role of Holocene relative sea-level change in preserving records of subduction zone earthquakes by Dura, Tina, Engelhart, Simon E., Vacchi, Matteo, Horton, Benjamin Peter, Kopp, Robert E., Peltier, William Richard, Bradley, Sarah

“…In near-field regions that were located beneath northern hemisphere ice sheets (e.g., western Vancouver Island), RSL fall from a mid-Holocene highstand has limited prehistoric earthquake records to the last 1 ka. In intermediate field regions (e.g., southern Washington and central Oregon), gradual RSL rise over the last ∼7 ka has preserved widespread records of prehistoric earthquakes. …”
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React based web-application for stock price forecasting with a novel stacking algorithm by Mittal, Madhav

“…The model performances are compared against a threshold performance of the “Last Value” algorithm. The “Last Value” algorithm is a naïve algorithm that assumes zero volatility in the data and thus sets all forecasts to be the value of the last observation. …”
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