Electrochemical scrutinizing techniques on initial development of pseudomonas aeruginosa strain (PAO1) biofilms by Foo, Yu Hao

“…The accumulation of biofilms on the surface of industrial equipment is natural yet often detrimental. In clinical settings, biofilms forming on medical implants pose health risks for patients with compromised immune systems; biofilms release a variety of virulence factors and are extremely resistant to antibiotics. …”
Get full text

Growth of myxococcus xanthus in continuous-flow-cell bioreactors as a method for studying development by Smaldone, Gregory T., Jin, Yujie, Whitfield, Damion L., Mu, Andrew Y., Wong, Edward C., Wuertz, Stefan, Singer, Mitchell

“…To demonstrate the versatility of our growth environment, we carried out time-lapse confocal laser scanning microscopy to visualize M. xanthus biofilm growth and fruiting body development, as well as fluorescence staining of exopolysaccharides deposited by biofilms. …”
Get full text
Get full text

Stomach tissue classification using autofluorescence spectroscopy and machine learning by Lim, Soo Y., Yoon, Hong M., Kook, Myeong-Cherl, Jang, Jin I., So, Peter T. C., Kang, Jeon W., Kim, Hyung M.

“…Abstract Background and objectives Determination of stomach tumor location and invasion depth requires delineation of gastric histological structure, which has hitherto been widely accomplished by histochemical staining. In recent years, alternative histochemical evaluation methods have been pursued to accelerate intraoperative diagnosis, often by bypassing the time-consuming step of dyeing. …”
Get full text

Conditional knock out of N-WASP in keratinocytes causes skin barrier defects and atopic dermatitis-like inflammation by Kalailingam, Pazhanichamy, Tan, Hui Bing, Jain, Neeraj, Sng, Ming Keat, Chan, Jeremy Soon Kiat, Tan, Nguan Soon, Thanabalu, Thirumaran

“…Neural-Wiskott Aldrich Syndrome Protein (N-WASP) is expressed ubiquitously and regulates actin cytoskeleton remodeling. In order to characterize the role of N-WASP in epidermal homeostasis and cutaneous biology, we generated conditional N-WASP knockout mouse using CK14-cre (cytokeratin 14) to ablate expression of N-WASP in keratinocytes. …”
Get full text
Get full text

Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells by Tan, Jerome, Chen, Jiahui, Roxby, Daniel, Chooi, Wai H., Nguyen, Tan D., Ng, Shi Y., Han, Jongyoon, Chew, Sing Y.

“…Despite its merits, iPSC differentiation into spinal cord progenitor cells (SCPCs) is variable, necessitating reliable assessment of differentiation and validation of cell quality and safety. Phenotyping is often performed via label-based methods including immunofluorescent staining or flow cytometry analysis. …”
Get full text

Correlative light and electron microscopy of telomeric repeat-binding factor 1 in mammalian cells by von Otter, Eric

“…Chromatin fibers range from 5 nm to 30 nm in diameter with a mean diameter of 18.5 nm which decreases to 13.1 nm in low salt conditions. In order to establish a comparative data-set of telomeric and non-telomeric chromatin, nucleosomes were stained for CLEM by eGFP_APEX2 labelling of H2B. …”
Get full text
Get full text

Construction and characterization of a Lactococcus lactis in-trans surface display system harboring murine glycosylated tyrosinase related protein-2 by Kalyanasundram, Jeevanathan

“…Codon optimized mtrp-2 and cA genes also did not result in target protein production. In order to investigate post-translational modification interruption, expression of codon optimized mtrp-2125-276 epitope devoid of mtrp-2 native maturation signal peptide was performed which resulted in misfolded plus aggregated mTRP-2125-276 and mTRP-2125-276 –cA protein production. …”
Get full text

Multi-spectral polarimetry imaging for early gastric cancer diagnosis by Wang, Wenfeng

“…This dissertation presents several studies in the development of polarimetry imaging techniques for gastric cancer diagnosis in order to approach the eventual goal of the detection of early gastric cancer in vivo in real time. …”
Get full text