Structural and molecular characterization of ligands activating the orphan nuclear receptor Nurr1 (NR4A2) by Ng, Xin Yi

“…Extending from previous studies done by our lab, small molecule ligands were designed to target a plausible co-regulator binding pocket which was unveiled following L1 binding on the ligand binding domain (LBD) of Nurr1. …”
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Protein arginine methyltransferase 6 enhances ligand-dependent and -independent activity of estrogen receptor α via distinct mechanisms by Sun, Yang, Chung, Hwa Hwa, Woo, Amanda Rui En, Lin, Valerie C.-L.

“…Instead, PRMT6 competes with Hsp90 for ERα binding: PRMT6 and Hsp90 bindings to ERα are mutually exclusive and PRMT6 over-expression reduces ERα interaction with Hsp90. …”
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The KDEL receptor by Bräuer, P

“…</p> <p><em>In vivo</em> cell localisation and <em>in vitro</em> binding assays were developed to examine different mutants of the KDELR and test hypotheses generated from structural data. …”

Development of high-affinity peptide-drug conjugates for investigating the TRIM24 PHD and bromodomain by Platt, MA

“…Although, no significant antiproliferative effects were observed in the ER⁺ MCF-7 breast cancer cell line, the triple negative CAL-51 cell line appeared sensitive to simultaneous TRIM24 PHD and BRD inhibition. …”

Transport and inhibition of the sphingosine-1-phosphate exporter SPNS2 by Li, HZ, Pike, ACW, Chang, Y, Prakaash, D, Gelova, Z, Stanka, J, Moreau, C, Scott, HC, Wunder, F, Wolf, G, Scacioc, A, McKinley, G, Batoulis, H, Mukhopadhyay, S, Garofoli, A, Pinto-Fernández, A, Kessler, BM, Burgess-Brown, NA, Štefanić, S, Wiedmer, T, Dürr, KL, Puetter, V, Ehrmann, A, Khalid, S, Sauer, DB

“…Here, we use a combination of cryo-electron microscopy, immunofluorescence, in vitro binding and in vivo S1P export assays, and molecular dynamics simulations to probe SPNS2’s substrate binding and transport. …”

Investigating molecular vulnerabilities in TP53-mutant transformed myeloproliferative neoplasms by Paterson, A

“…MPN are a heterogenous spectrum of stem cell-derived neoplasm defined by excess clonal proliferation of mature myeloid entities. …”

Integrated transcriptomic and proteomic profiling of responses to clinical and novel bisphosphonates by Lu, J

“…This DPhil thesis aim to comprehensively investigate bisphosphonates by exploring their cellular uptake and subcellular localization, associated genomic and proteomic changes, effects on cell growth and senescence, binding targets, and undiscovered mechanisms of action. …”

Surface modification using layer-by-layer method for antimicrobial applications by Sim, Sherlyne Xiu Hui.

“…RGD promotes the specific binding between host cell and biomedical devices; it should not have a significant effect on bacterial adhesion.…”
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Structural features that affect β2 integrin expression and regulation of adhesion by Guan, Siyu

“…(It should be noted that wild-type LFA-1 requires one activating agent to bind ICAM-1 and two to bind ICAM-3.) …”
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Observation of E-cadherin adherens junction dynamics with metal-induced energy transfer imaging and spectroscopy by Chen, T, Karedla, N, Enderlein, J

“…Epithelial cadherin (E-cad) mediated cell-cell junctions play a crucial role in the establishment and maintenance of tissues and organs. …”

Structure and function of atypical BTB domains in health and disease by Cruz Walma, DA

“…Novel 3D cell culture models alongside several microscopy and proteomic assays revealed BTBD7 may contribute to <em>in vitro</em> cellular phenotypes consistent with tubular morphogenesis that were not observed <em>in vivo</em>, perhaps due to incomplete knockout of the gene. …”

Hybrid nanomaterials for improved photocatalytic performance by Ho, Jeffrey Weng Chye.

“…The redox reactions will regenerate continuously while the composition of the photocatalyst will remain unchanged. Till date, titanium dioxide (TiO2) is the most commonly used photocatalyst. …”
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Genetic analysis of over 1 million people identifies 535 new loci associated with blood pressure traits by Evangelou, Evangelos, Warren, Helen R., Mosen-Ansorena, David, Mifsud, Borbala, Pazoki, Raha, Gao, He, Ntritsos, Georgios, Dimou, Niki, Cabrera, Claudia P., Ibrahim Karaman, Ng, Fu Liang, Evangelou, Marina, Witkowska, Katarzyna, Tzanis, Evan, Hellwege, Jacklyn N., Giri, Ayush, Edwards, Digna R. Velez, Sun, Yan V., Cho, Kelly, Gaziano, J. Michael, Wilson, Peter W. F., Tsao, Philip S., Kovesdy, Csaba P., Esko, Tonu, Mägi, Reedik, Milani, Lili, Almgren, Peter, Boutin, Thibaud, Debette, Stéphanie, Ding, Jun, Giulianini, Franco, Holliday, Elizabeth G., Jackson, Anne U., Li-Gao, Ruifang, Lin, Wei-Yu, Luan, Jian'an, Mangino, Massimo, Oldmeadow, Christopher, Prins, Bram Peter, Qian, Yong, Sargurupremraj, Muralidharan, Shah, Nabi, Surendran, Praveen, Thériault, Sébastien, Verweij, Niek, Willems, Sara M., Zhao, Jing-Hua, Amouyel, Philippe, Connell, John, de Mutsert, Renée, Doney, Alex S. F., Farrall, Martin, Menni, Cristina, Morris, Andrew D., Raymond Noordam, Paré, Guillaume, Poulter, Neil R., Shields, Denis C., Stanton, Alice, Thom, Simon, Abecasis, Gonçalo, Najaf Amin, Arking, Dan E., Ayers, Kristin L., Barbieri, Caterina M., Batini, Chiara, Bis, Joshua C. M., Blake, Tineka, Bochud, Murielle, Boehnke, Michael, Boerwinkle, Eric, Boomsma, Dorret I., Bottinger, Erwin P., Braund, Peter S., Brumat, Marco, Campbell, Archie, Campbell, Harry, Chakravarti, Aravinda, Chambers, John Campbell, Chauhan, Ganesh, Ciullo, Marina, Cocca, Massimiliano, Collins, Francis, Cordell, Heather J., Davies, Gail, de Borst, Martin H., de Geus, Eco J., Deary, Ian J., Deelen, Joris, Del Greco M., Fabiola, Demirkale, Cumhur Yusuf, Dörr, Marcus, Ehret, Georg B., Elosua, Roberto, Enroth, Stefan, Erzurumluoglu, A. Mesut, Ferreira, Teresa, Frånberg, Mattias, Franco, Oscar H., Gandin, Ilaria, Gasparini, Paolo, Giedraitis, Vilmantas, Gieger, Christian, Girotto, Giorgia, Goel, Anuj, Gow, Alan J., Gudnason, Vilmundur, Guo, Xiuqing, Gyllensten, Ulf, Hamsten, Anders, Harris, Tamara B., Harris, Sarah E., Hartman, Catharina A., Havulinna, Aki S., Hicks, Andrew A., Hofer, Edith, Hofman, Albert, Hottenga, Jouke-Jan, Huffman, Jennifer E., Hwang, Shih-Jen, Ingelsson, Erik, James, Alan, Jansen, Rick, Jarvelin, Marjo-Riitta, Joehanes, Roby, Johansson, Åsa, Johnson, Andrew D., Joshi, Peter K., Jousilahti, Pekka, Jukema, J. Wouter, Jula, Antti, Kähönen, Mika, Kathiresan, Sekar, Keavney, Bernard D., Khaw, Kay-Tee, Knekt, Paul, Knight, Joanne, Kolcic, Ivana, Kooner, Jaspal S., Koskinen, Seppo, Kristiansson, Kati, Kutalik, Zoltan, Laan, Maris, Larson, Marty, Launer, Lenore J., Lehne, Benjamin, Lehtimäki, Terho, Liewald, David C. M., Lin, Li, Lind, Lars, Lindgren, Cecilia M., Liu, YongMei, Loos, Ruth J. F., Lopez, Lorna M., Lu, Yingchang, Lyytikäinen, Leo-Pekka, Mahajan, Anubha, Mamasoula, Chrysovalanto, Marrugat, Jaume, Marten, Jonathan, Milaneschi, Yuri, Morgan, Anna, Morris, Andrew P., Morrison, Alanna C., Munson, Peter J., Nalls, Mike A., Nandakumar, Priyanka, Nelson, Christopher P., Niiranen, Teemu, Nolte, Ilja M., Nutile, Teresa, Oldehinkel, Albertine J., Oostra, Ben A., O'Reilly, Paul F., Org, Elin, Padmanabhan, Sandosh, Palmas, Walter, Palotie, Aarno, Pattie, Alison, Penninx, Brenda W. J. H., Perola, Markus, Peters, Annette, Polasek, Ozren, Pramstaller, Peter P., Nguyen, Quang Tri, Raitakari, Olli T., Ren, Meixia, Rettig, Rainer, Rice, Kenneth, Ridker, Paul M., Ried, Janina S., Riese, Harriëtte, Ripatti, Samuli, Robino, Antonietta, Rose, Lynda M., Rotter, Jerome I., Rudan, Igor, Ruggiero, Daniela, Saba, Yasaman, Sala, Cinzia F., Salomaa, Veikko, Samani, Nilesh J., Sarin, Antti-Pekka, Schmidt, Reinhold, Schmidt, Helena, Shrine, Nick, Siscovick, David, Smith, Albert V., Snieder, Harold, Sõber, Siim, Sorice, Rossella, Starr, John M., Stott, David J., Strachan, David P., Strawbridge, Rona J., Sundström, Johan, Swertz, Morris A., Taylor, Kent D., Teumer, Alexander, Tobin, Martin D., Tomaszewski, Maciej, Toniolo, Daniela, Traglia, Michela, Trompet, Stella, Tuomilehto, Jaakko, Tzourio, Christophe, Uitterlinden, André G., Vaez, Ahmad, van der Most, Peter J., van Duijn, Cornelia M., Vergnaud, Anne-Claire, Verwoert, Germaine C., Vitart, Veronique, Völker, Uwe, Vollenweider, Peter, Vuckovic, Dragana, Watkins, Hugh, Wild, Sarah H., Willemsen, Gonneke, Wilson, James F., Wright, Alan F., Yao, Jie, Zemunik, Tatijana, Zhang, Weihua, Attia, John R., Butterworth, Adam S., Chasman, Daniel I., Conen, David, Cucca, Francesco, Danesh, John, Hayward, Caroline, Howson, Joanna M. M., Laakso, Markku, Lakatta, Edward G., Langenberg, Claudia, Melander, Olle, Mook-Kanamori, Dennis O., Palmer, Colin N. A., Risch, Lorenz, Scott, Robert A., Scott, Rodney J., Sever, Peter, Spector, Tim D., van der Harst, Pim, Wareham, Nicholas J., Zeggini, Eleftheria, Levy, Daniel, Munroe, Patricia B., Newton-Cheh, Christopher, Brown, Morris J., Metspalu, Andres, Hung, Adriana M., O'Donnell, Christopher J., Edwards, Todd L., Psaty, Bruce M., Tzoulaki, Ioanna, Barnes, Michael R., Wain, Louise V., Elliott, Paul, Caulfield, Mark J.

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Interaction Analyses of the Integrin β2 Cytoplasmic Tail with the F3 FERM Domain of Talin and 14-3-3ζ Reveal a Ternary Complex with Phosphorylated Tail by Chatterjee, Deepak, Lu, Lewis Zhiping, Tan, Suet-Mien, Bhattacharjya, Surajit

“…Integrins, which are heterodimeric (α and β subunits) signal-transducer proteins, are essential for cell adhesion and migration. β cytosolic tails (β-CTs) of integrins interact with a number of cytosolic proteins including talin, Dok1, and 14-3-3ζ. …”
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Phosphatidylinositol 4,5-Bisphosphate Is an HCV NS5A Ligand and Mediates Replication of the Viral Genome by Cho, Nam-Joon, Lee, Choongho, Pang, Phillip S., Pham, Edward A., Fram, Benjamin, Nguyen, Khanh, Xiong, Anming, Sklan, Ella H., Elazar, Menashe, Koytak, Elif S., Kersten, Caroline, Kanazawa, Kay K., Frank, Curtis W., Glenn, Jeffrey S.

“…Methods: We used a combination of quartz crystal microbalance, circular dichroism, molecular genetics, and immunofluorescence to study specific binding of PI(4,5)P2 by the HCV NS5A protein. We evaluated the effects of PI(4,5)P2 on the function of NS5A by expressing wild-type or mutant forms of Bart79I or FL-J6/JFH-5’C19Rluc2AUbi21 RNA in Huh7 cells. …”
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Mechanisms that promote the evolution of cross-reactive antibodies upon vaccination with designed influenza immunogens by Yang, Leerang, Caradonna, Timothy M, Schmidt, Aaron G, Chakraborty, Arup K

“…We show that this result follows from an interplay between how B cells engage these antigens and interact with diverse helper T cells and requires T cell-mediated selection of germinal center B cells to be a stringent constraint. …”
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Specific inhibition of PI3K p110δ inhibits CSF‐1‐induced macrophage spreading and invasive capacity by Mouchemore, KA, Sampaio, N, Murrey, MW, Stanley, ER, Lannutti, BJ, Pixley, FJ

“…We have previously demonstrated that CSF‐1‐induced phosphorylation of Y721 (pY721) in the receptor kinase insert triggers its association with the p85 regulatory subunit of phosphoinositide 3′‐kinase (PI3K). Binding of p85 PI3K to the CSF‐1R pY721 motif activates the associated p110 PI3K catalytic subunit and stimulates spreading and motility in macrophages and enhancement of tumor cell invasion. …”

Roles of POPX2 phosphatase in the regulation of apoptosis by Weng, Ting

“…Apart from regulating cell morphology, higher substrate stiffness also increases cell growth. …”
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A university-based clinical approach to renewable energy facility siting in the United States by Susskind, Lawrence, Chun, Jungwoo, Beron, David, Chaudhuri, Anushree, Paul, Sanjana

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Synthesis, biological evaluation, and stability studies of raloxifene mono- and bis-sulfamates as dual-targeting agents by Zaraei, S-O, Dohle, W, Anbar, HS, El-Gamal, R, Leblond, B, Foster, PA, Al-Tel, TH, Potter, BVL, El-Gamal, MI

“…All three possible sulfamate derivatives of the selective estrogen receptor modulator Raloxifene (bis-sulfamate 7 and two mono-sulfamates 8–9) were synthesized and evaluated as inhibitors of the clinical drug target steroid sulfatase (STS), both in cell-free and in cell-based assays, and also as estrogen receptor (ER) modulators. …”