Structural basis of haptoglobin-haemoglobin scavenging by CD163 by Zhou, X

“…Indeed, biophysical assays show that calcium ions are critical for ligand binding. Mutational studies demonstrate that while monomeric CD163 can scavenge the multimeric Hp(2-2)Hb, uptake of the dimeric Hp(1-1)Hb and Hb is impaired compared to the wild-type receptor, suggesting that receptor multimerisation evolved to facilitate uptake of lower affinity ligands. …”

Recognition of A-U and U-A base pairs in RNA duplexes by modified peptide nucleic acids by Yuan, Zhen

“…Fluorescence binding studies were carried out to measure the binding affinity at physiological pH conditions. …”
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Discovery of novel ligands using phage display by Nur Azri Muhamad Azan

“…Cellular interactions with their surroundings are pivotal for cells to function. These interactions are facilitated by a number of receptors found on the surface of cells, bound to specific glycoproteins found in the extracellular matrix (ECM). …”
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The role of toca-1-N-wasp complex in filopodia formation and endocytosis by Bu, Wenyu

“…Toca-1 or N-WASP induces neurite outgrowth and filopodia formation in N1E115 cells. Toca-1 requires the F-BAR domain, Cdc42 binding site and SH3 domain to induce filopodia. …”
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Surface modified polymers for controlled macroscopic functions by Chng, S

“…In particular, drug delivery properties were investigated using hole-plate bioassays and HPLC-MS methods, and it was found that the antibiotic, Penicillin V, was still active after being loaded onto the polymer scaffolds, with surface modified polymers binding and releasing more drug than the corresponding controls. …”

The study of radiotherapy resistance of hypoxic tumour for improving survival of cancer patient. by Goh, Yeek Teck.

“…Despite the advent of many new targeted cancer therapies, radiotherapy is still considered an important treatment modality in killing localised cancer cells. …”
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Application of COLD-PCR for improved detection of EGFR mutations in clinical samples. by Zhang, Zhi Xuan.

“…Due to the significance of mutation status of the epidermal growth factor receptor (EGFR) gene in non-small-cell lung cancer (NSCLC) affecting patient response to tyrosine kinase inhibitors (TKI), the more sensitive and robust the method used to detect the mutations, the better. …”
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Surface composition dependent ligand effect in tuning the activity of nickel–copper bimetallic electrocatalysts toward hydrogen evolution in alkaline by Wei, Chao, Sun, Yuanmiao, Scherer, Günther G., Fisher, Adrian C., Sherburne, Matthew, Ager, Joel W., Xu, Zhichuan Jason

“…Unfortunately, the principles that governs alkaline HER performance remain unclear and are still under debate. By studying the alkaline HER at a series of NiCu bimetallic surfaces, where the electronic structure is modulated by ligand effect, we demonstrate that alkaline HER activity can be correlated with either the calculated or the experimental-measured d band center (an indicator of hydrogen binding energy) via a volcano-type relationship. …”
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Genetic and molecular characterisation of non-tissue culture adapted influenza B viruses detected in Singapore between 2004-2009 by Muhammad Raihan Jumat

“…Even so, the mechanism yielding this peptide is still unknown since caspase and MMP inhibitors did not have an effect in its expression. …”
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Synthesis and application of peptidoglycan oligomers as metabolic labeling agents for bacteria by He, Jingxi

“…In the following part, mechanistic studies further supported the hypothesis of enzyme mediated metabolic labeling rather than non-specific binding to bacterial surface. The cell wall-deficient L-form strain of enterococcus showed a drastic reduction in PGO-rhodamine incorporation, and calorimetric studies further confirmed the strong binding between our PGOs and the penicillin-binding protein 1a (PBP1a). …”
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Structure and inhibition of novel cyclin-dependent kinases by Dixon-Clarke, S

“…I also identified rebastinib and dabrafenib as potent, clinically-relevant inhibitors of CDK16 and solved a co-crystal structure that defined the extended type II binding mode of rebastinib. Preliminary trials using these relatively non-selective compounds to inhibit CDK16 in melanoma and medulloblastoma cancer cell lines revealed rebastinib as the more efficacious drug causing loss of cell proliferation in the 1-2 micromolar range. …”

The Long Noncoding RNA RMST Interacts with SOX2 to Regulate Neurogenesis by Ng, Shi-Yan, Bogu, Gireesh K., Soh, Boon Seng, Stanton, Lawrence Walter

“…Through RNA interference and genome-wide SOX2 binding studies, we found that RMST is required for the binding of SOX2 to promoter regions of neurogenic transcription factors. …”
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Structural and molecular characterization of ligands activating the orphan nuclear receptor Nurr1 (NR4A2) by Ng, Xin Yi

“…Extending from previous studies done by our lab, small molecule ligands were designed to target a plausible co-regulator binding pocket which was unveiled following L1 binding on the ligand binding domain (LBD) of Nurr1. …”
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Protein arginine methyltransferase 6 enhances ligand-dependent and -independent activity of estrogen receptor α via distinct mechanisms by Sun, Yang, Chung, Hwa Hwa, Woo, Amanda Rui En, Lin, Valerie C.-L.

“…Instead, PRMT6 competes with Hsp90 for ERα binding: PRMT6 and Hsp90 bindings to ERα are mutually exclusive and PRMT6 over-expression reduces ERα interaction with Hsp90. …”
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The KDEL receptor by Bräuer, P

“…</p> <p><em>In vivo</em> cell localisation and <em>in vitro</em> binding assays were developed to examine different mutants of the KDELR and test hypotheses generated from structural data. …”

Unconventional split aptamers cleaved at functionally essential sites preserve biorecognition capability by Wang, Ruoyu, Zhang, Qiansen, Zhang, Yi, Shi, Hanchang, Nguyen, Kim Truc, Zhou, Xiaohong

“…An allosteric binding mechanism of BPSPAs, in which a new binding pocket is formed upon the target binding, is revealed by all-atom microsecond-scale molecular dynamics simulations. …”
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Development of high-affinity peptide-drug conjugates for investigating the TRIM24 PHD and bromodomain by Platt, MA

“…Although, no significant antiproliferative effects were observed in the ER⁺ MCF-7 breast cancer cell line, the triple negative CAL-51 cell line appeared sensitive to simultaneous TRIM24 PHD and BRD inhibition. …”

Transport and inhibition of the sphingosine-1-phosphate exporter SPNS2 by Li, HZ, Pike, ACW, Chang, Y, Prakaash, D, Gelova, Z, Stanka, J, Moreau, C, Scott, HC, Wunder, F, Wolf, G, Scacioc, A, McKinley, G, Batoulis, H, Mukhopadhyay, S, Garofoli, A, Pinto-Fernández, A, Kessler, BM, Burgess-Brown, NA, Štefanić, S, Wiedmer, T, Dürr, KL, Puetter, V, Ehrmann, A, Khalid, S, Sauer, DB

“…Here, we use a combination of cryo-electron microscopy, immunofluorescence, in vitro binding and in vivo S1P export assays, and molecular dynamics simulations to probe SPNS2’s substrate binding and transport. …”

Investigating molecular vulnerabilities in TP53-mutant transformed myeloproliferative neoplasms by Paterson, A

“…MPN are a heterogenous spectrum of stem cell-derived neoplasm defined by excess clonal proliferation of mature myeloid entities. …”

Integrated transcriptomic and proteomic profiling of responses to clinical and novel bisphosphonates by Lu, J

“…This DPhil thesis aim to comprehensively investigate bisphosphonates by exploring their cellular uptake and subcellular localization, associated genomic and proteomic changes, effects on cell growth and senescence, binding targets, and undiscovered mechanisms of action. …”