Discovery of stabilized functional mutants of human aquaporin 1 by Soh, Miao Yi

“…A cell-based freeze-thaw functionality assay that was developed by the lab was conducted on yeast Saccharomyces cerevisiae cells transformed with the plasmids to identify the functional mutants, i.e., mutants that express the mutated AQP1 protein that would allow them to exhibit a protective effect in response to rapid freezing. ~ 5% of mutants from a total of 2816 screened from thirty-three 96-well plates were identified after a series of initial and verification screens. …”
Get full text

Optimization of carbon and nitrogen sources for phytase production by Mitsuokella jalaludinii, a new rumen bacterial species by Lan, G.Q., Abdullah, N., Jalaludin, S., Ho, Y.W.

“…Methods and Results: Replacement of glucose, cellobiose and starch in MF1 medium by RB or palm kernel cake and replacement of trypticase peptone and yeast extract in the medium by SM or enzymatic digested soybean milk significantly increased the phytase production by M. jalaludinii. …”
Get full text

Phase separation of a microtubule plus-end tracking protein into a fluid fractal network by Czub, MP, Uliana, F, Grubić, T, Padeste, C, Rosowski, KA, Lorenz, C, Dufresne, ER, Menzel, A, Vakonakis, I, Gasser, U, Steinmetz, MO

“…Here, we have study the phase separation of Bik1, a CLIP-170 family member and key +TIP involved in budding yeast cell division. Bik1 is a dimer with a rod-shaped conformation primarily defined by its central coiled-coil domain. …”

Modelling pH dynamics, SCOBY biomass formation, and acetic acid production of Kombucha fermentation using black, green, and Oolong Teas by Chong, Ann Qi, Chin, Nyuk Ling, Talib, Rosnita A., Basha, Roseliza Kadir

“…Kombucha is a traditional, fermented beverage made with an essential biomaterial known as SCOBY (symbiotic culture of bacteria and yeast). Three different tea types, namely black, green, and oolong, were compared in kombucha fermentation in terms of pH dynamics, the formation of SCOBY biomass, and the production of acetic acid. …”
Get full text

Application of scoby bacterial cellulose as hydrocolloids on physicochemical, textural and sensory characteristics of mango jam by Chong, Ann Qi, Chin, Nyuk Ling, A. Talib, Rosnita, Kadir Basha, Roseliza

“…BACKGROUND: The scoby pellicle of symbiotic culture of bacteria and yeast is a by-product from kombucha fermentation. …”

Temporal Telepresence: Immersive Interfaces for TeleAbsence by Pillis, D.

“…To store the past in a simulation may enable greater understanding of ourselves, our stories, and our histories. …”
Get full text

Development of fed-batch strategy to produce bacteria cellulose by Chua, Gek Kee, Soo, Yang Kee

“…First, batch fermentation mode was carried out to determine the glucose consumption profile of Gluconacetobacter xylinus using Yeast Extract Glucose Chloramphenicol Medium (YGC). …”
Get full text

Molecular characterization of Candida auris outbreak isolates in Qatar from patients with COVID-19 reveals the emergence of isolates resistant to three classes of antifungal drugs by Abid, Fatma Ben, Salah, Husam, Sundararaju, Sathyavathi, Dalil, Lamya, Abdelwahab, Ayman H., Salameh, Sarah, Ibrahim, Emad B., Almaslmani, Muna A., Tang, Patrick, Perez-Lopez, Andres, Tsui, Clement Kin-Ming

“…During the COVID-19 pandemic in Qatar, many patients who were severely ill were colonized and infected by Candida auris, an invasive multidrug-resistant yeast pathogen that spreads through nosocomial transmission within healthcare facilities. …”
Get full text

Legionella pneumophila filamentous morphological form and the generation of novel spherical form upon interaction with protozoan hosts by Chan, Sock Hoai

“…Our investigation showed that filamentation cannot be neatly pegged to a single dominant factor but is promoted under a concerted influence of environmental conditions leaning towards (i) higher than ambient temperatures, at 37-42°C, (ii) static fluid movement, and (iii) with at least a minimal organic content of 5% buffered yeast extract, or equivalent. In the course of investigating interaction of the filamentous form with protozoan hosts, a spherical form was discovered. …”
Get full text

Characterization of the near native conformational states of the SAM domain of Ste11 protein by NMR spectroscopy by Gupta, Sebanti, Bhattacharjya, Surajit

“…SAM domain of the Ste11 protein of budding yeast plays important roles in mitogen-activated protein kinase cascades. …”
Get full text
Get full text

On the total synthesis of complex alkaloids by Shennan, BDA

“…Following a concerted investigation into C−H oxidation for the former and a transfer of chirality approach for the latter, a Baker’s yeast reduction is adopted as an efficient route towards an enantioenriched, oxidised pyrrolidine building block which is advanced to a pentacyclic intermediate.…”

The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice by Carvalho, P, Gomes, C, Gonçalves, I, Lourenço, TF, Vlad, D, Langdale, JA, Saibo, NJM

“…To engineer C₄ into a C₃ plant, it is essential to understand how C₄ genes, such as <i>phosphoenolpyruvate carboxylase (PEPC1)</i>, are regulated to be expressed at high levels and in a cell-specific manner.</li> <li>Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the <i>Setaria viridis PEPC1</i> promoter. …”

Recent insight into the advances and prospects of microbial lipases and their potential applications in industry by Eskandari, Azadeh, Thean, Chor Leow, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…This updated review presents new knowledge about various origins of microbial lipases, such as fungi, bacteria, and yeast. It highlights both the traditional and modern purification methods, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, the aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF), moreover, delves into the diverse applications of microbial lipases across several industries, such as food, vitamin esters, textile, detergent, biodiesel, and bioremediation. …”

CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome by Dalvie, Neil C., Lorgeree, Timothy R., Yang, Yuchen, Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J., Del Rosario, Amanda M., Love, Kerry R., Love, J. C.

“…Background The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. …”
Get full text

Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site by Banach, Bailey B, Pletnev, Sergei, Olia, Adam S, Xu, Kai, Zhang, Baoshan, Rawi, Reda, Bylund, Tatsiana, Doria-Rose, Nicole A, Nguyen, Thuy Duong, Fahad, Ahmed S, Lee, Myungjin, Lin, Bob C, Liu, Tracy, Louder, Mark K, Madan, Bharat, McKee, Krisha, O’Dell, Sijy, Sastry, Mallika, Schön, Arne, Bui, Natalie, Shen, Chen-Hsiang, Wolfe, Jacy R, Chuang, Gwo-Yu, Mascola, John R, Kwong, Peter D, DeKosky, Brandon J

“…Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. …”
Get full text

Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. …”
Get full text
Get full text

Finding transcription factor binding motifs for coregulated genes by combining sequence overrepresentation with cross-species conservation by Jia, Hui., Li, Jinming.

“…Compared with the prevailing motif finding software, our method has some advantages in finding transcriptional factor binding motifs for potential coregulated genes if the gene upstream sequences of multiple closely related species are available. Although we used yeast genomes to assess our method in this study, it might also be applied to other organisms if suitable related species are available and the upstream sequences of coregulated genes can be obtained for the multiple closely related species.…”
Get full text
Get full text

Niemann-Pick type c disease: pathogenesis and therapy by Colaco, A

“…To gain further insight into the function of NPC1 and proteins it interacts with we used the yeast orthologue, Ncr1p. I recreated the Δncr1 mutant and characterized the mutant using an array of systematic screens to identify different processes and pathways that may play a role in NPC pathogenesis. …”

Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods by Mohd Yusof, Rokiah, Haque, Formuzul, Ismail, Maznah, Hassan, Zaiton

“…Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. …”

Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants by Eskandari, Azadeh, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. …”