Discovery of novel ligands using phage display by Nur Azri Muhamad Azan

“…Recombinant laminin-globular 3 (rLG3) from laminin-5 was developed and purified in order to be used to study the binding affinity with α3β1 and α6β4 via ELISA test. However, the test showed there was insignificant optical density against the concentration of α6β4. …”
Get full text

Development of a novel colorimetric immunoassay for C-Reactive Protein using colloidal nanoparticles by Tjitra, Niklas Lesmana

“…In this project, colorimetric gold nanoparticle assays based on local surface plasmon coupling phenomena were developed using anti-CRP antibodies and affimer proteins. ELISA experiments were first performed to confirm the binding of antibodies and affimer proteins to CRP. …”
Get full text

Power management system design and optimization for European Student Moon Orbitor (ESMO) by Muhammad Dzahir Mohamed Zain Affandi.

“…ESMO is a European Space Agency (ESA) initiative to involve the universities to build the first student satellite to orbit the Moon. …”
Get full text

Part geometry-driven crystallographic texture control in a 3D-printed austenitic steel – a strategy for near-monocrystalline microstructure generation by Chandra, Shubham, Tan, Xipeng, Kumar, Punit, Ramamurty, Upadrasta

“…Strikingly, the strength of the 〈001〉 || z crystallographic texture and columnar grain width with increasing prism angle is seen to follow a ‘bell-curve’ profile which peaks with the X-90 shape. …”
Get full text

Systemic interrogation of immune-oncology-related proteins in patients with locally advanced prostate cancer undergoing androgen deprivation and intensity-modulated radiotherapy by Guldvik, IJ, Ramberg, H, Kristensen, G, Røder, A, Mills, IG, Lilleby, W, Taskén, KA

“…PurposeThe primary objective was to establish whether blood-based leucine-rich alpha-2-glycoprotein (LRG1) can predict outcomes in patients with locally advanced prostate cancer undergoing androgen-deprivation therapy (ADT) and radiotherapy (RT) and to determine how it may relate to 92 immune-oncology (I-O)-related proteins in this setting.MethodsBaseline blood level of LRG1 from patients treated with ADT and RT enrolled in the CuPCa (n = 128) and IMRT (n = 81) studies was measured using ELISA. A longitudinal cohort with matched blood samples from start of ADT, start of RT, and end of RT protocol from 47 patients from the IMRT cohort was used to establish levels of I-O proteins by high-multiplexing Proximal Extension Assay by Olink Proteomics. …”

Characterization of immune response and duration of protection in buffaloes immunized with haemorrhagic septicaemia vaccines by Chandrasekaran, S., Kennett, L., Yeap, P.C., Muniandy, N., Rani, B., Mukkur, T.K.S.

“…These results suggested that there was a relationship between ELISA antibody titres and active protection in buffaloes. …”
Get full text

Mechanism of damnacanthal induced apoptosis in CEM-SS cell line by Gopalsamy, Banulata, Saiful Yazan, Latifah, Abdul Hamid, Hisyam

“…Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. …”
Get full text

Development and application of novel proteomics methods in proteomics research by Hao, Piliang

“…The existing challenges for accurately identifying and quantifying protein deamidation sites in proteomics include tremendous false positive identifications of deamidated peptides from database searches, substantial interference of artificial deamidation from sample preparation, difficulties in differentiating and quantifying the isomeric n-Asp and isoAsp deamidation products in proteome scale, and inability of validating quantification of deamidated peptides by either ELISA or multiple reaction monitoring. In this thesis, novel proteomic methods were developed to overcome these challenges and facilitate the large-scale characterization of protein deamidation. …”
Get full text

Removal of noxious environmental pollutant using photocatalytic membrane reactor by Yeap, Ken Vin.

“…The average roughness of the membrane is 10nm with a very well distributed (Gaussian bell curve) pore size. The technique used in this project was able to achieve uniformity on the skin layer, as the band gap remains the same which membrane coated with multiple layers. …”
Get full text

Airway proteomics reveals broad residual anti-inflammatory effects of prednisolone in mepolizumab-treated asthma by Howell, I, Yang, F, Brown, V, Cane, J, Marchi, E, Azim, A, Busby, J, McDowell, PJ, Diver, SE, Borg, C, Heaney, LG, Pavord, ID, Brightling, CE, Chaudhuri, R, Hinks, TSC

“…We also analysed additional sputum proteins using ELISA. <p><b>Results</b></p> In patients receiving mepolizumab, prednisolone significantly downregulated sputum proteins related to type-2 inflammation and chemotaxis including IL-4, IL-5, IL-13, CCL24, CCL26, EDN, CCL17, CCL22, OX40 receptor, FCER2, and the ST2 receptor. …”

Immunological screening of Lassa virus among health workers and contacts of patients of Lassa fever in Ondo State by Shaibu, JO, Salu, OB, Amoo, OS, Idigbe, I, Musa, AZ, Ezechi, OC, Abejegah, C, Ayodeji, O, Salako, BL, Omilabu, SA, Audu, RA

“…Plasma samples were analyzed for IgG and IgM markers using ReLASV® Pan-Lassa NP IgG/IgM ELISA Kit (Zalgen Labs, LLC, USA) while RNAs extracted from IgM positive samples were analyzed for LASV RNA according to manufacturers’ instructions. …”

Inhibition of nitric oxide production in RAW 264.7 cells and cytokines IL-1β in osteoarthritis rat models of 70 % ethanol extract of Arcangelisia flava (L.) merr stems by Pratama, Rizki Rahmadi, Sari, Riza Ambar, Sholikhah, Irawati, Mansor, Hakiman, Chang, Hsin-I, Sukardiman, Widyowati, Retno

“…The NO inhibition test was determined by the NO colorimetric assay using Griess reagent and measured by the ELISA plate reader. The measurement of joint diameter and hyperalgesia in osteoarthritis rats was carried out once a week for 7 weeks, and then the IL-1β levels were measured at weeks 3 and 7. …”
Get full text

Intraperitoneal injection of the Porphyromonas gingivalis outer membrane vesicle (OMV) stimulated expressions of neuroinflammatory markers and histopathological changes in the brai... by Adewoyin, Malik, Hamarsha, Ahmed, Akinsola, Rasaq, Teoh, Seong Lin, Azmai, Mohammad Noor Amal, Abu Bakar, Noraini, Nasruddin, Nurrul Shaqinah

“…Brains of experimental zebrafish were dissected at desired time points for colorimetric assays, ELISA, and histology. This study discovered that nitric oxide and PGE2 were significantly increased at 45 min, while IL-1β and IL-6 were expressed at subsequent 12 h and 24 h time points, respectively. …”
Get full text

Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma by Lyu, Zhenlei, Mahenderan, Appukutty, Radhakrishnan, Ammu Kutty G. K., Chin, Yit Siew, Yin, Chao

“…CCK-8 and colony formation assays were used to detect cell viability and proliferation. ELISA was employed to determine the levels of cytokines secreted by macrophages. …”
Get full text

Assessment of phagocytosis and cytokine secretions by monocytes in the presence of plasmodium falciparum by Xuan, Keh Min

“…falciparum, to assess phagocytic capability of infected red blood cells by human monocytes and further measure the cytokine secretions of monocytes following phagocytosis by using ELISA. In this study, monocytes were isolated from whole blood collected from healthy individuals while Plasmodium falciparum (3D7) was cultured under optimal conditions. …”
Get full text

Effects of transport inhibitors on the cellular uptake of liposomes from mycobacterium smegmatis by human monocytes dendritic cells by Nordin, Nor Asyikin

“…Supernatant will be collected to measure the cytokine level by ELISA. Therefore, in this proposed study, we are going to fill up the gaps by investigating the uptake mechanisms of liposomes-Msmeg as a potent lipid-based vaccine by DCs from PBMC in healthy individuals.…”
Get full text

Global monitoring of volcanic SO2 degassing with unprecedented resolution from TROPOMI onboard Sentinel-5 Precursor by Barrington, Charlotte, Taisne, Benoit, Theys, N., Hedelt, P., De Smedt, I., Lerot, C., Yu, H., Vlietinck, J., Pedergnana, M., Arellano, S., Galle, B., Fernandez, D., Carlito, C. J. M., Delgado-Granados, H., Loyola, D., Van Roozendael, M.

“…We report here the first volcanic SO2 measurements from the hyperspectral TROPOspheric Monitoring Instrument (TROPOMI) launched in October 2017 onboard the ESA’s Sentinel-5 Precursor platform. Using the operational processing algorithm, we explore the benefit of improved spatial resolution to the monitoring of global volcanic degassing. …”
Get full text
Get full text

Towards a Single Bio-molecule Detector Based on CMOS Nanofluidic Platform by Zikrallah, Ahmed S.

“…Ideally, we would be able to continuously monitor the secretome of these cell therapies as they are transformed and expanded in manufacturing.However, state-of-theart techniques for monitoring typically low concentrations of cytokines require either Mass Spectroscopy (MS) or immunoassays like Enzyme-linked Immunosorbent Assay (ELISA). We propose the use of CMOS technology to build a proteomic platform with a single biomolecule resolution. …”
Get full text
Get full text

Skin dendritic cell and T cell activation associated with dengue shock syndrome by Duyen, Huynh Thi Le, Cerny, Daniela, Trung, Dinh The, Pang, Jassia, Velumani, Sumathy, Toh, Ying Xiu, Qui, Phan Tu, Hao, Nguyen Van, Simmons, Cameron, Haniffa, Muzlifah, Wills, Bridget, Fink, Katja

“…We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. …”
Get full text
Get full text

The chronic cerebral hypoperfusion model induces proinflammatory cascades in Alzheimer's disease by Abedi, Zahra, Basri, Hamidon, Hassan, Zurina, Inche Mat, Liyana Najwa, Khaza’ai, Huzwah, Mohd Ali, Razana

“…The gene expression levels of cathepsin B, NLRP3, an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 were analysed using real-time PCR, while the expression levels of the inflammatory cytokines were estimated using the ELISA method. Structural damage to the hippocampus was assessed using hematoxylin and eosin (HE) staining. …”
Get full text