High-resolution HDX-MS reveals distinct mechanisms of RNA recognition and activation by RIG-I and MDA5 by Liu, Chuan-Fa, Zheng, Jie, Yong, Hui Yee, Panutdaporn, Nantika, Tang, Kai, Luo, Dahai

“…Here we employ hydrogen/deuterium exchange coupled with mass spectrometry (HDX-MS) to probe the apo states of RIG-I and MDA5 and to dissect the molecular details with respect to distinct RNA species recognition, ATP binding and hydrolysis and CARDs activation. …”
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Nutraceuticals silybin B, resveratrol, and epigallocatechin-3 gallate-bind to cardiac muscle troponin to restore the loss of lusitropy caused by cardiomyopathy mutations in vitro,... by Yang, Z, Sheehan, AM, Messer, AE, Tsui, S, Sparrow, A, Redwood, C, Kren, V, Gould, IR, Marston, SB

“…Methods: We did molecular dynamics simulations (5x1500ns for each condition) of the unphosphorylated and phosphorylated cardiac troponin core with the G159D DCM mutation in the presence of the 5 ligands and analysed the effects on several dynamic parameters. …”

Mixed lead-tin perovskite films with >7 μs charge carrier lifetimes realized by maltol post-treatment by Hu, S, Truong, MA, Otsuka, K, Handa, T, Yamada, T, Nishikubo, R, Iwasaki, Y, Saeki, A, Murdey, R, Kanemitsu, Y, Wakamiya, A

“…Maltol, a naturally occurring flavor enhancer and strong metal binding agent, was found to effectively suppress Sn(IV) formation and passivate defects in mixed Pb–Sn perovskite films. …”

Solution X-ray scattering studies on the dengue virus non-structural protein 5 and -3 as well as the capsid protein by Saw, Wuan Geok

“…Upon adding RNA into the NS5 solution, an increase in size of NS5 was detected using SAXS. …”
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Enantiomeric separation of racemic mixtures using chiral-selective and organic-solvent-resistant thin-film composite membranes by Ong, Chi Siang, Oor, Jia Zheng, Tan, Jonathan Shiliang, Chew, Jia Wei

“…In this study, we fabricated a novel thin-film composite nanofiltration membrane composed of (2-hydroxypropyl)-beta-cyclodextrin (HP-β-CD) as the chiral selector for the enantiomeric separation of racemic 1-phenylethanol chiral compounds in organic solvents. …”
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HLA-DQB1*05 subtypes and not DRB1*10:01 mediates risk in anti-IgLON5 disease by Yogeshwar, SM, Muñiz-Castrillo, S, Sabater, L, Peris-Sempere, V, Mallajosyula, V, Luo, G, Yan, H, Yu, E, Zhang, J, Lin, L, Fagundes Bueno, F, Ji, X, Picard, G, Rogemond, V, Pinto, AL, Heidbreder, A, Höftberger, R, Graus, F, Dalmau, J, Santamaria, J, Iranzo, A, Schreiner, B, Giannoccaro, MP, Liguori, R, Shimohata, T, Kimura, A, Ono, Y, Binks, S, Mariotto, S, Dinoto, A, Bonello, M, Hartmann, CJ, Tambasco, N, Nigro, P, Prüss, H, McKeon, A, Davis, MM, Irani, SR, Honnorat, J, Gaig, C, Finke, C, Mignot, E

“…The functional relevance of the observed HLA-DQ molecules was studied with competition binding assays. These proof-of-concept experiments revealed preferential binding of IgLON5 in a post-translationally modified, but not native, state to all three risk-associated HLA-DQ receptors. …”

The Mark of Cain: The crime of terrorism in times of armed conflict as interpreted by the Court of Appeal of England and Wales in R v. Mohammed Gul by Coco, A

A KLF2-BMPER-Smad1/5 checkpoint regulates high fluid shear stress-mediated artery remodeling by Deng, H, Zhang, J, Wang, Y, Joshi, D, Pi, X, De Val, S, Schwartz, MA

“…Here we report that high FSS suppresses Smad1/5 by elevating KLF2, which induces the bone morphogenetic protein (BMP) pathway inhibitor, BMP-binding endothelial regulator (BMPER), thereby de-inhibiting Akt. …”

High-density lipoprotein function is modulated by the SARS-CoV-2 spike protein in a lipid-type dependent manner by Correa, Yubexi, Del Giudice, Rita, Waldie, Sarah, Thépaut, Michel, Micciula, Samantha, Gerelli, Yuri, Moulin, Martine, Delaunay, Clara, Fieschi, Franck, Pichler, Harald, Haertlein, Michael, Forsyth, V. Trevor, Le Brun, Anton, Moir, Michael, Russell, Robert A., Darwish, Tamim, Brinck, Jonas, Wodaje, Tigist, Jansen, Martin, Martín, César, Roosen-Runge, Felix, Cárdenas, Marité

“…It is known that the SARS-CoV-2 spike (S) protein binds the HDL particle, probably depleting it of lipids and altering HDL function. …”
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The SARS-CoV-2 nucleocapsid protein interferes with the full enzymatic activation of UPF1 and its interaction with UPF2 by Nuccetelli, V, Mghezzi-Habellah, M, Deymier, S, Roisin, A, Gérard-Baraggia, F, Rocchi, C, Coureux, P, Gouet, P, Cimarelli, A, Mocquet, V, Fiorini, F

“…Additionally, we showed that Np binds directly to UPF2, disrupting the formation of the UPF1/UPF2 complex essential for NMD progression. …”

A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53 by Wong, Jin Huei, Alfatah, Mohammad, Sin, Mei Fang, Sim, Hong May, Verma, Chandra Shekhar, Lane, David P., Arumugam, Prakash

“…Using the p53-Mdm2 interaction to optimize the assay, we show that the p53-Mdm2 inhibitor nutlin-3 is a substrate for the yeast ATP-binding cassette (ABC) transporter Pdr5. By deleting nine ABC transporter-related genes, we generated a ABC9Δ yeast strain that is highly permeable to small molecules. …”
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Zeolitic imidazolate frameworks activate endosomal Toll-like receptors and potentiate immunogenicity of SARS-CoV-2 spike protein trimer by Alsaiari, Shahad K., Nadeef, Seba, Daristotle, John L., Rothwell, William, Du, Bujie, Garcia, Johnny, Zhang, Linzixuan, Sarmadi, Morteza, Forster, Timothy A., Menon, Nandita, Lin, Stacey Qiaohui, Tostanoski, Lisa H., Hachmann, Nicole, Wang, Erika Yan, Ventura, John D., Barouch, Dan H., Langer, Robert, Jaklenec, Ana

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Homology modeling and expression of recombinant NS5-RDRP based on the Indonesian local strain of dengue virus for anti-dengue drug development by Subroto, Toto, Firdaus, Ade Rizqi Ridwan, Kusumawardani, Shinta, Tohari, Taufik Ramdani, Nababan, Theresia Adventina, Pertiwi, Wulan, Fadhlillah, Muhammad, Kusuma, Sri Agung Fitri, Rukayadi, Yaya

“…As a result, the RdRp gene, which belongs to the non-structural protein 5 (NS5) of DENV, was successfully isolated and sequenced from DENV RNA samples. …”
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Optical properties of ultrathin metal oxide and metal films : effect of free electrons, quantum confinement and localized surface plasmon resonance by Li, Xiao Dong

“…The evolution of the dielectric function is shown related to the changes in the interband absorption, discrete-exciton absorption, and continuum-exciton absorption, which can be attributed to the quantum confinement effect on both the band gap and exciton binding energies. Thickness dependence of optical properties of a-IGZO thin films has also been observed. …”
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Understanding the molecular mechanism of ion and amino acid transport in neurons by Baroniņa, A

“…I used X-ray crystallography to determine the structures of TREK-2 with bound activatory and inactivatory nanobodies at 2.4 Å and 3.5 Å resolution, respectively. Both nanobodies bind to the channel from the extracellular side but interact with different domains to elicit the opposite effects on the channel conductivity. …”

Myopathy-causing Q147P TPM2 mutation shifts tropomyosin strands further towards the open position and increases the proportion of strong-binding cross-bridges during the ATPase cyc... by Karpicheva, OE, Simonyan, AO, Kuleva, NV, Redwood, CS, Borovikov, YS

“…Wild-type and Q147P recombinant tropomyosins, actin, and myosin subfragment-1 were modified by 5-IAF, 1,5-IAEDANS or FITC-phalloidin, and 1,5-IAEDANS, respectively, and incorporated into single ghost muscle fibers, containing predominantly actin filaments which were free of troponin and tropomyosin. …”

Effects of free electrons and quantum confinement in ultrathin ZnO films : a comparison between undoped and Al-doped ZnO by Li, X. D., Liu, P., Liu, Y., Leong, K. C., Chen, Tupei

“…Band gaps and exciton binding energies of undoped and Al-doped ZnO thin films were determined from optical absorption measurement based on the Elliott’s exciton absorption theory. …”
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Utilization of biomass pectin polymer to build high efficiency electrode architectures with sturdy construction and fast charge transfer structure to boost sodium storage performan... by Zhao, Jing, Yang, Xu, Zhang, Yu, Loh, Xian Jun, Hu, Xiaodong, Chen, Gang, Du, Fei, Yan, Qingyu

“…Thus, the sturdy construction enables Na₃V₂(PO₄)₃/C (NVP) to run for over 15 000 cycles. …”
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Imprinting of IgA responses in previously infected individuals receiving bivalent mRNA vaccines (WT and BA.4/BA.5 or WT and BA.1) by Goh, Yun Shan, Fong, Siew-Wai, Hor, Pei Xiang, Loh, Chiew Yee, Tay, Matthew Zirui, Wang, Bei, Salleh, Siti Nazihah Mohd, Ngoh, Eve Zi Xian, Lee, Raphael Tze Chuen, Poh, Xuan Ying, Lee, I. Russel, Rao, Suma, Chia, Po Ying, Maurer-Stroh, Sebastian, Wang, Cheng-I, Leo, Yee Sin, Lye, David C., Young, Barnaby Edward, Ng, Lisa F. P., Renia, Laurent

“…Results: We found significantly lower fold change in serum binding IgA responses against BA.1, BA.5 and EG.5.1 spike in the bivalent booster group, compared with the monovalent (WT) booster group, following vaccination. …”
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Clinical course, immunogenicity, and efficacy of BNT162b2 mRNA vaccination against SARS-CoV-2 infection in liver transplant recipients by Tan, Eunice X., Lim, Wen Hui, Thong, Elizabeth, Chavatte, Jean-Marc, Zhang, Jinyan, Lim, Jonathan, Jin, Jocelyn Y., Lim, Daniel R. X., Kang, Jaclyn Y. T., Tang, Ansel Shao Pin, Chan, Kai En, Tan, Caitlyn, Tan, Shi Ni, Nah, Benjamin, Huang, Daniel Q., Wang, Lin-Fa, Tambyah, Paul A., Somani, Jyoti, Young, Barnaby Edward, Muthiah, Mark D.

“…We measured the antibody response against the SARS-CoV-2 spike protein using the Roche Elecsys immunoassay to the receptor-binding domain of the SARS-CoV-2 spike protein, and the presence of neutralizing antibodies was measured by the surrogate virus neutralization test (cPass) before first and second doses of vaccination and also between 2 and 3 mo after the second dose of vaccination. …”
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