Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…Compared to the human FKBP12−FK506 complex reported earlier, the structure reveals structural differences in the β5−β6 segment that lines the FK506 binding site. The presence in PfFKBD of Cys-106 and Ser-109 (substituting for His-87 and Ile-90, respectively, in human FKBP12), which are 4−5 Å from the nearest atom of the FK506 compound, suggests possible routes for the rational design of analogues of FK506 with specific antiparasitic activity. …”
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Functional comparison of human Adenomatous Polyposis Coli (APC) and APC-like in targeting beta-catenin for degradation by Schneikert, Jean, Chandra, Shree Harsha Vijaya, Ruppert, Jan Gustav, Ray, Suparna, Wenzel, Eva Maria, Behrens, Jürgen

“…We show here that colon cancer cell lines express also the paralog APC-like (APCL or APC2). …”
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Clonal phylogenies inferred from bulk, single cell, and spatial transcriptomic analysis of epithelial cancers by Erickson, A, Figiel, S, Rajakumar, T, Rao, S, Yin, W, Doultsinos, D, Magnussen, A, Singh, R, Poulose, N, Bryant, RJ, Cussenot, O, Hamdy, FC, Woodcock, D, Mills, IG, Lamb, AD

“…We first performed in-silico comparisons of inferred and directly resolved SNV and CNV status, from single cancer cells, from three different cell lines. We found that inferred SNV phylogenies accurately recapitulate DNA phylogenies (entanglement = 0.097). …”

Performance, heterosis and heritability in selected tropical maize single, double and three-way cross hybrids by Saleh, G.B., Abdullah, D., Anuar, A.R.

“…A series of tropical maize hybrids, involving 10 single, four double and four three-way crosses were evaluated with their parental inbred lines and three control varieties, at Universiti Putra Malaysia, Serdang, Malaysia, in a randomized complete block design, with three replications. …”
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When the Earth Breathes: An Anthology of Volcanic Urbanism by Carucci Alvarez, Maria Gabriela

“…Igneous landscapes, through the production of new basalt floors, rich soils and ocean intrusions, traverse and redefine property boundary lines and national coastlines, which extends beyond plan views and into sectional shifts. …”
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Concept-level sentiment analysis in the manufacturing industry by Teng, He Xu

“…Manufacturers can make use of this technology to improve quality control, standardization and maintenance through producing predictive analyzes of equipment functionality and radically streamlining factory lines. Manufacturing will continue to be an important pillar of Singapore’s economy. …”
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Diameter-free estimates for the quadratic Vinogradov mean value theorem by Mudgal, A

“…Consequently, our strategy differs from these two lines of approach, and we employ techniques from incidence geometry, arithmetic combinatorics and analytic number theory. …”

Ritual speech, performativity, and modality by Frangulov, AM

“…The incorporation of historical data from the ritual corpora of the Rigveda and Hittite ritual tablets, has not only enriched the quality of analysis through quantitative analysis, but it also provided catalysts for many of the discussions raised and many future lines of inquiry. In the examination of the facts of performativity in ritual speech, this thesis has shown that formal semantic consideration of this type of data can reveal many insights into performative speech as a whole. …”

Src family kinase expression and subcellular localization in macrophages: implications for their role in CSF‐1‐induced macrophage migration by Dwyer, AR, Mouchemore, KA, Steer, JH, Sunderland, AJ, Sampaio, N, Greenland, EL, Joyce, DA, Pixley, FJ

“…We report a comprehensive analysis of individual Src family kinase expression in macrophage cell lines and primary macrophages and demonstrate colony‐stimulating factor‐1–induced changes in Src family kinase subcellular localization, which provides clues to their distinct and redundant functions in macrophages. …”

Investigating the inner controls on explosive eruptions using textural analyses and petrology by Bernard, Olivier

“…Second I use apatite to finely study the volatile budgets and degassing rates of the magma batches that fed the sub-plinian, effusive and vulcanian phases of the 2006 Rabaul eruption. …”
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Studies on high-frequency noise characteristics in deep submicron NMOSFETs by Zeng, Rong

“…The reduction of minimum noise figure with the increase in body bias may provide a possible methodology to finely adjust the device high-frequency noise performance for circuit design.…”
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Augmented Object Intelligence with XR-Objects by Dogan, Mustafa Doga, Gonzalez, Eric, Ahuja, Karan, Du, Ruofei, Cola?o, Andrea, Lee, Johnny, Gonzalez-Franco, Mar, Kim, David

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Site-specific chemical and topological modifications to augment mRNA therapeutic potential by Aditham, Abhishek J.

“…We show that mocRNAs are subject to slower deadenylation and enhance therapeutic protein expression in cell lines and primary cell cultures. We expanded on this technology by creating mRNAs with chemically branched poly(A) tails, or multitail mRNAs, which increase the density of modifications at the 3’ end of mocRNA and further stabilize mRNA against deadenylation. …”
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Rhetorical contestation and the mediterranean migration crisis, 2013-2018 by He, Wendy Qingli

“…In addition, I argue that the securitization of the migration crisis is perpetuated by the EU and Italy’s use of complicity to construct a political narrative that blurs the lines of responsibility and culpability. By ascribing complicity to others using highly politicized rhetoric, the EU and Italy complicate the narrative of the migration crisis, making it more challenging to attribute responsibility to any one party. …”
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Engineering a surface to enhance endothelial cell adhesion towards creating a 'roll-up' blood vessel prosthesis by Chua, Hui Yee.

“…The optimized gelatin “ink” allowed extended viability for the entrapped HUVECs and the drawn scaffold lines to retain their integrity whilst in growth media. …”
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In vitro and in silico investigation of TRPC3 mechanism and dynamics by Collingridge, J

“…This work provides computational evidence for the ability of TRPC3 to form a widely conserved, mechanistically important π-helix structural component in the pore-lining S6 helix. This has not previously been reported for TRPC3, and simulations presented here suggest a role for the π-helix in promoting a transition towards an open state of the channel. …”

Psoriatic Arthritis Priority Setting Partnership: patient and clinician informed considerations for future UK health service delivery by James, L, Hailey, L, Bundy, C, Burstow, H, Chandler, D, Cowper, R, Helliwell, P, Joannes, L, Kelly, A, Kennedy, B, Kinsella, S, McAteer, H, Mukherjee, S, Packham, J, Wise, E, Young, H, Dures, E, Coates, LC

“…Further analysis within the rheumatology service theme revealed additional sub-themes that emphasised integrating multidisciplinary services, improving access to advice lines and ensuring fair access to treatments.</p> <br> <p><strong>Conclusion:</strong> The five key themes provide valuable insights into the important areas of interest within health service delivery in the UK. …”

Investigating the contribution of the unfolded protein response to prostate cancer bone metastasis by Kaya, Z

“…Using paired androgen refractory cancer of the prostate (ARCaP) cell lines ARCaPE and ARCaPM which differ in their epithelial (E) and mesenchymal (M) characteristics, I found that components of IRE1 and ATF6 ER stress pathways are higher in ARCaPE cells than in ARCaPM and decreased upon osteogenic differentiation of ARCaPM cells. …”

Engineering control of rhizobacteria for plant growth promotion by Green, PWL

“…The rhizopine system was used to demonstrate tight transcriptional control of the NifA master transcriptional regulator of <em>nif</em> genes for nitrogen fixation <em>in vitro</em>, thereby developing a rhizopine responsive diazotrophic strain which can be assayed for plant growth promotion on rhizopine producing barley lines. To test the feasibility of controlled synthetic symbiosis in a nodule environment, rhizopine control of nitrogen fixation was assayed in the model symbiotic rhizobium <em>S. meliloti</em>. …”

Elevated reactive oxygen species can drive the alternative lengthening of telomeres pathway in ATRX-null cancers by Goncalves, T, Cunniffe, S, Ma, T, Mattis, N, Rose, A, Kent, T, Mole, D, Geiller, HB, van Bijsterveldt, L, Humphrey, T, Hammond, E, Gibbons, R, Clynes, D, Rose, A

“…We also demonstrate that elevation of ROS can trigger over-activity of the ALT pathway in osteosarcoma and glioma cell lines, resulting in excessive DNA damage and cell death. …”