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2221
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2222
Establishment of optimise strategies in production of recombinant Hepatitis B surface antigen for chip-based antibody detection approach
Published 2015“…The main results drawn showed that the IEC Q Sepharose XL column and optimum conditions of 4 mL sample loading volume, an elution buffer with 1.5 M NaCl concentration at pH 8 with an elution gradient length of 20 column volume and subsequent purification with SEC at a sample volume of 5 mL and a flow rate of 1 mL/min was able to efficiently purify HBsAg. An up-scaled version the established purification strategy comprising of the two techniques further demonstrated adaptability for scale-up operations with a final total PF of 94.82 ± 16.20, HBsAg purity of 95.48% and recovery yield of 78.07%. …”
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Thesis -
2223
Dysregulation of the Pdx1/Ovol2/Zeb2 axis in dedifferentiated β-cells triggers the induction of genes associated with epithelial-mesenchymal transition in diabetes
Published 2022“…We carried out loss- and gain-of-function experiments in MIN6 and EndoC-bH1 cell lines. We analysed previously published mouse andhuman T2D data sets.Results:Bulk RNA-seq revealed thatb-cell dedifferentiation is associated with the induction of genes associated with epithelial-to-mesenchymal transition (EMT) in prediabetic (2-week-old) and diabetic (12-week-old) Tg7 mice. …”
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Journal Article -
2224
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2225
Conformational design characterization of a truncated diamine oxidase
Published 2014“…By maintaining the native protein’s pH profile, the mini protein was highly active at 40 °C and stable at pH 7, with a half-life of 80 min at 50 °C. Results from the kinetic studies showed that the Km value for native diamine oxidase was 0.274 mM and 1.3 mM for the mini protein. …”
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Thesis -
2226
Tropical peat subsidence, nutrient losses and oil palm seedling growth due to different water table depths
Published 2018“…The system was made of exchange chamber of air pump with the flow rate ranges between 1 and 3.5 L min-1, exchange chamber of (2670 mL plastic container) and a trap (250 mL Erlenmeyer flask). …”
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Thesis -
2227
HiPIMS deposition of VO2 thermochromic coating for enhanced optical properties
Published 2022“…In chapter 4, a HiPIMS deposition technique for VO2(M) thin film on a commercial material, such as soda-lime glass substrate, was established with a high deposition rate of 5.7 nm/min. The VO2(M) deposited on soda-lime glass exhibits excellent crystallinity and thermochromic properties (highest luminous transmission Tlum ≈ 30.4%, and solar modulation ΔTsol ≈ 12%) in comparison with similarly prepared VO2 on high temperature glass and quartz substrates. …”
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Thesis-Doctor of Philosophy -
2228
Evaluation of paper spray mass spectrometry for direct analysis of small molecule drugs and hemoglobin constant spring peptide markers
Published 2016“…Further application of the PS-MS assay to preclinical pharmacokinetic (PK) study found that, the assay was simple and rapid with overall analysis time of 1 min and a full plasma concentration vs. time profile of sunitinib could be obtained from a single mouse. …”
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Thesis -
2229
Antimicrobial activity, phytochemical and toxicity analyses of salam [Syzygium polyanthum (Wight) Walp.] leaf extract and its application in food
Published 2018“…Generally the significant reduction of natural microflora in tested food samples were started at 0.50% (v/v) of extract at 5 min. During storage, 5% (v/v) showed better effect in controlling the microbial survival throughout the storage time. …”
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Thesis -
2230
The prospective associations of 24-hour movement behaviors and domain-specific activities with executive function and academic achievement among school-aged children in Singapore
Published 2024“…However, higher moderate-to-vigorous PA (MVPA) relative to remaining movement behaviors at age 5.5 years was associated with lower academic achievement [Mean difference (95% confidence interval): −0.367 (−0.726, −0.009) z-score], and reallocating MVPA time to sleep showed higher academic achievement scores [30 min from MVPA to sleep: 0.214 (0.023, 0.404) z-score]. …”
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Journal Article -
2231
The iHealth-T2D study, prevention of type 2 diabetes amongst South Asians with central obesity and prediabetes: study protocol for a randomised controlled trial
Published 2022“…Usual care comprised a single 30-min session of lifestyle modification advice from the community health worker. …”
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Journal Article -
2232
Genetic analysis of over 1 million people identifies 535 new loci associated with blood pressure traits
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Journal Article -
2233
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2234
Exploring the stability and dynamics of nanobubbles
Published 2017“…These nanobubbles appear not to be stabilised by the line pinning or oversaturation that sustain surface nanobubbles. …”
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Thesis -
2235
Effect of dopant solubility and excess doping on the superconducting properties of doped Nb3Sn prepared by field assisted sintering technique
Published 2024“…The Y<sub>2</sub>O<sub>3</sub> particles was also considered as a possible cause of pinning down of grain boundaries in addition to NbO leading to grain refinement through Zener pinning. …”
Journal article -
2236
Cross-sectional analysis of university students’ health using a digitised health survey
Published 2021“…Areas of concern were highlighted in student’s body weight, visual acuity, and binge drinking. A large proportion of students were underweight (body mass index (BMI) < 18.5)—18.9% of females and 10.6% of males—and 7% of males were obese (BMI > 30). …”
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Journal Article -
2237
Defending, contesting and rejecting formal drinker categories: how UK university students identify as ‘light-drinkers’ or ‘non-drinkers’
Published 2021“…To explore this, we presented self-identified ‘non’ or ‘light’ drinkers with official formal definitions of ‘light’ and ‘binge’ drinking as found in public health and academic research. …”
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Article -
2238
Spintronics devices for neuromorphic computing
Published 2020“…To solve this, we discuss the possibility of using a geometrically pinned magnetic domain wall memory device that utilises anti-notches for pinning, as the free layer of an MTJ, for future use in a three-terminal MRAM. …”
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Final Year Project (FYP) -
2239
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2240
Transverse domain wall profile for spin logic applications
Published 2015“…The topological defects of Transverse DWs (TDW) are of paramount importance as regards to the deterministic pinning and movement of DW within complex networks of conduits. …”
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Journal Article