Optimizing Cas9-mediated HDR in HEK293 by Lee, Cheryl

“…This project aims to improve HDR efficiency, by increasing proximity of ideal donor repair template to repair site via fusing donor plasmid to gRNA. Our findings show that plasmid donors are able to achieve a consistent HDR efficiency.…”
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A computer vision and smartphone-based tool for onsite color correction and dimensional measurements by Xiong, Wenxi

“…This project aims to develop a portable and low-cost solution which is capable of accurately measuring objects in 3-dimensional (3D) space and providing on-site color correction functionality under varying lighting conditions. …”
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Development of a lab on a chip for nerve agent detection by Tan, Hsih Yin

“…An escalating number of threats in terrorist activities had led to an urgent demand for innovative devices for on-site detection of chemical and biological agents as well as explosive materials. …”
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Effect of WuWeiXiaoDuYin (五味消毒饮) and its components on LFA-1 mediated leukocyte adhesion to ICAM-1. by Tan, Kang Ting.

“…Leukocytes circulate as non-adherent cells and are recruited to the specific site of inflammation after the activation of cell adhesion molecules by pro-inflammatory mediators and cytokines. …”
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Scene text detection using neural network by Tan, Yi Hong

“…The second is to use supervised pre-training followed by domain-specific finetuning to coaching giant convolutional neural networks once the tagged training information is lean [1]. we tend to train a replacement model supported a dataset collected by ourselves from google web site. In this project, we tend to first of all introduce some relevant ideas. we tend to describe the selective search technique used for region proposal, and also the compositions of building blocks of convolutional neural network (CNN) that embrace convolutional layer, pooling layer, corrected linear units (ReLU), fully-connected layer and loss layer. we tend to detail the method of information set preparation that consists of data assortment, information labelling and format transformation, that is taken into account as vital preparation work for coaching method.The core part of this project is that the institution of the scene text detection system from the module style to the model testing and validation.…”
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Structural basis of RNA binding by leucine zipper GCN4 by Nikolaev, Yaroslav, Pervushin, Konstantin

“…Solution NMR experiments showed that the LZ-RNA interaction interface is located in the first two heptads of LZ moiety, and that only the dimeric (coiled coil) LZ conformation is capable of binding RNA. Site-directed mutagenesis of the LZ-GCN4 RNA binding interface showed that substrate binding is facilitated by lysine and arginine side chains, and that at least one nucleophilic residue is located in proximity to the RNA phosphate backbone. …”
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BugSigDB captures patterns of differential abundance across a broad range of host-associated microbial signatures by Geistlinger, L, Mirzayi, C, Zohra, F, Azhar, R, Elsafoury, S, Grieve, C, Wokaty, J, Gamboa-Tuz, SD, Sengupta, P, Hecht, I, Ravikrishnan, A, Gonçalves, RS, Franzosa, E, Raman, K, Carey, V, Dowd, JB, Jones, HE, Davis, S, Segata, N, Huttenhower, C, Waldron, L

“…We present BugSigDB, a community-editable database of manually curated microbial signatures from published differential abundance studies accompanied by information on study geography, health outcomes, host body site and experimental, epidemiological and statistical methods using controlled vocabulary. …”

Targeted mutagenesis of zebrafish antithrombin III triggers disseminated intravascular coagulation and thrombosis, revealing insight into function by Liu, Y, Kretz, CA, Maeder, ML, Richter, CE, Tsao, P, Vo, AH, Huarng, MC, Rode, T, Hu, Z, Mehra, R, Olson, ST, Joung, JK, Shavit, JA

“…Mutation of the human AT3-reactive center loop abolished the ability to rescue, but the heparin-binding site was dispensable. These results demonstrate overall conservation of AT3 function in zebrafish, but reveal developmental variances in the ability to tolerate excessive clot formation. …”

Paracrine provision of lipids in the immune system by Pond, C

“…By supplying appropriate fatty acids to lymph nodes and dendritic cells, perinodal adipocytes intervene between the diet and the immune systems nutrition and partially emancipate it from fluctuations in abundance and composition of dietary lipids. Site-specific differences in the composition of lipids taken up from contiguous adipocytes create further dendritic cell diversity. …”

Environmental change and connectivity drive coral reef fish abundance in the Western Indian Ocean by Warmuth, LM, Kelly, S, Samoilys, MA, Popova, E, Head, CEI, Bonsall, MB

“…We designed a proportional oceanographic connectivity metric describing the relative strength of connectivity between all WIO coral reefs and each survey site. We created statistical models for four trophic groups: grazers and detritivores, herbivorous excavators, corallivores, and primary piscivores across 51 sites in the WIO. …”

“But some were more equal than others:” exploring inequality at Neolithic Çatalhöyük by Twiss, KC, Bogaard, A, Haddow, S, Milella, M, Taylor, JS, Veropoulidou, R, Kay, K, Knüsel, CJ, Tsoraki, C, Vasić, M, Pearson, J, Busacca, G, Mazzucato, C, Pochron, S

“…We perceive broadly similar access to staple foods, but not to goods less directly related to survival; relatively elevated income inequality during the middle portion of the site’s occupation, plausibly deliberately tamped down; and no evidence for institutionalized or lasting economic or social inequality. …”

Object retrieval with large vocabularies and fast spatial matching by Philbin, J, Chum, O, Isard, M, Sivic, J, Zisserman, A

“…We demonstrate the scalability and performance of our system on a dataset of over 1 million images crawled from the photo-sharing site, Flickr [3], using Oxford landmarks as queries. …”

Numerical modeling of the influence of scour and scour protection on monopile dynamic behavior by Mayall, RO, Burd, HJ, McAdam, RA, Byrne, BW, Whitehouse, RJS, Heald, SG, Slater, PL

“…The calibrated 1D model is applied to a field case study at a UK offshore wind farm site.…”

Opportunity in lost spaces: exploring the potential of edible landscapes in viaduct underpasses by Yang, Huihua, Hussein, Mohd Kher, Ibrahim,, Roziya, Zhuang, Qianda

“…Using a case study approach that involves on-site observations, questionnaires, and photographic documentation, this study will document the characteristics of 29 space nodes beneath 18 viaducts and along their routes in Guangzhou’s Tianhe District. …”
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Evaluating efficiency of nondestructive detection of flaws in concrete by Sutan, N. Mohamed, Jaafar, M.S.

“…Results of our tests indicate that both methods can be used in determining depths of flaws in concrete and in performing on-site tests. The accuracy of both methods proved to be better in tests of stronger concretes, and the detection probability ranged from 51.81 to 99.8% after aging during 3 to 28 days under full load, respectively.…”

Driven Factors to Sustainable Paddy Farming Production in Malaysia by Zahari, Fadhilah, Othman, Zakirah, Abu, Noor Hidayah, Salleh, Nurul Azita

“…Using interview as data collection method, case samples were selected from various SRI paddy site in Kedah. The findings indicated that implementing SRI practices in organic paddy cultivation had caused the value chain to be different from conventional paddy value chain in terms of actor and effect of middle man subject to the small scale paddy production. …”
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Developments in THz Polaritonics: Towards Integrated Nonlinear THz Spectroscopy by Sung, Eric Rueyhao

“…In a typical polaritonics experiment, the optical pump light is focused to a single line which launches THz fields with electric field strengths of approximately 10 kV/cm. …”
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Using cluster analysis to reconstruct dengue exposure patterns from cross-sectional serological studies in Singapore by Sangkaew, Sorawat, Tan, Li Kiang, Ng, Lee Ching, Ferguson, Neil M., Dorigatti, Ilaria

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Serotype distribution and incidence of invasive early onset and late onset group B streptococcal disease amongst infants in Singapore by Kam, Kai-Qian, Thoon, Koh Cheng, Tee, Nancy Wen Sim, Ang, Michelle Lay Teng, Tan, Natalie Woon Hui, Yeo, Kee Thai, Li, Jiahui, Chong, Chia Yin

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Germline breast cancer susceptibility genes, tumor characteristics, and survival by Ho, Peh Joo, Khng, Alexis J., Loh, Hui Wen, Ho, Weang-Kee, Yip, Cheng Har, Mohd-Taib, Nur Aishah, Tan, Veronique Kiak Mien, Tan, Benita Kiat-Tee, Tan, Su-Ming, Tan, Ern Yu, Lim, Swee Ho, Jamaris, Suniza, Sim, Yirong, Wong, Fuh Yong, Ngeow, Joanne, Lim, Elaine Hsuen, Tai, Mei Chee, Wijaya, Eldarina Azfar, Lee, Soo Chin, Chan, Ching Wan, Buhari, Shaik Ahmad, Chan, Patrick M. Y., Chen, Juliana J. C., Seah, Jaime Chin Mui, Lee, Wai Peng, Mok, Chi Wei, Lim, Geok Hoon, Woo, Evan, Kim, Sung-Won, Lee, Jong Won, Lee, Min Hyuk, Park, Sue K., Dunning, Alison M., Easton, Douglas F., Schmidt, Marjanka K., Teo, Soo-Hwang, Li, Jingmei, Hartman, Mikael

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