A microfluidic platform to study pathogen-host interactions at single cell level by Zhang, Rui

“…Single bacterium detection with the success rates of 90% for P. aeruginosa and 94% for S. aureus can be achieved with good reproducibility using the optimized DNA isolation protocol. Association profiling of P. aeruginosa and S. aureus in single infection context to A549 cells at three time points revealed different adherence patterns of these two pathogens. …”
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Cryo-EM structure and evolutionary history of the conjugation surface exclusion protein TraT by Seddon, C, David, S, Wong, JLC, Ishimoto, N, He, S, Bradshaw, J, Low, WW, Frankel, G, Beis, K

“…Following transfer of IncF-like plasmids, recipients become refractory to a second wave of conjugation with the same plasmid via entry (TraS) and surface (TraT) exclusion mechanisms. …”

Structural edge detection of photographic images of rooms with machine learning by Yao, Xin Meng

“…Future works include further enhancing the current model’s accuracy and creating an algorithm to construct a wireframe model from the line equations.…”
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The design of a CMOS only low-power low-voltage low-dropout voltage regulator (LDO) with an embedded voltage reference by Xie, Xinzhe

“…A recycling folded cascode (RFC) amplifier achieves higher loop gain, improving load and line regulation. A recovery time enhancement circuit reduces overshoot recovery. …”
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Mimo: multipath signals and location aware by Luo, Yong Bin.

“…In this report, experimental study was conducted to investigate the multipath effect and the improvement to the signal quality by measuring the QoS parameters between MIMO (802.11n) and SISO (802.11a/b/g) under line of sight (LOS) and non-line of sight (NLOS) environment. …”
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A low-cost dual output switch mode power supply (SMPS) for laptop by Palaniappan Balathandayutham

“…It switches at a much-higher frequency 40 kHz than that of the AC line, which means that the transformer that it feeds can be much smaller than one connected directly to the line/mains. …”
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Thermodynamics of hairy black holes in Lovelock gravity by Hennigar, Robie A., Tjoa, Erickson, Mann, Robert B.

“…We also report on a number of novel results, including ‘virtual triple points’ and the first example of a ‘λ-line’ — a line of second order phase transitions — in black hole thermodynamics.…”
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Specific inhibition of PI3K p110δ inhibits CSF‐1‐induced macrophage spreading and invasive capacity by Mouchemore, KA, Sampaio, N, Murrey, MW, Stanley, ER, Lannutti, BJ, Pixley, FJ

“…While primary bone marrow‐derived macrophages and the immortalized bone marrow‐derived macrophage cell line M−/−.WT express all three class IA PI3K isoforms, p110δ predominates in the cell line. …”

Single cell analysis at the nanoscale by Zheng, Xin Ting, Li, Chang Ming

“…The fundamental life processes such as signal transduction, intracellular trafficking, protein degradation, and DNA repair often occur in nanometric subcellular compartments. …”
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Contribution of metabolic alterations to genome instability and its association with cancer development by Woo, Ren He

“…These nuclear abnormalities were found to be caused by DNA double stranded breaks (DSBs). It was discovered that phosphorylated ATR and total Chk1 were deregulated through the proteosomal degradation pathway, which could have caused cells to become susceptible to DSBs via replication stress. …”
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Conception of conjugative cloning vectors. by Tan, Peng Fei.

“…Unlike conventional methods of cloning genes, which usually involve the use of restriction enzymes to clone large gene fragment into the target organism, our triparental system can override this laborious process using simple genetic tools constructed synthetically. Combining DNA sequencing technology with conjugation protocols, tri-parental mating was performed using 3 different Escherichia coli strains, S17-1 strain as the donor of a pUC vector with RP4 OriT incorporated, a PHL 628 strain possessing the ~7.8kb large fragment(MalA gene) and lastly a S17-1 Rifamycin-resistant strain being the collector of the recombinant shuttle plasmid. …”
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Display of chikungunya epitope on ferritin for potential application as vaccine by Su, Chengxun

“…In comparison with the existing widely-used technology of vaccine production where the epitope and carrier protein are separately produced and subsequently coupled together, the novelty of this approach is the one-step production of the whole piece of vaccine by integrating the epitope with protein cage at DNA level. Ferritin is a ubiquitous protein produced by almost all living organisms. …”
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Potential of serum in creation of small-diameter vascular graft by Lim, Wee Keng

“…Our data suggest a further reduction in DNA and α-gal content from FBS- and HS-mediated decellularized PCA. …”
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Stem cell genome-to-systems biology by Chia, Na Yu, Ng, Huck-Hui

“…With the elucidation of protein–protein interaction, protein–DNA regulation, microRNA involvement as well as the epigenetic modifications, it is possible to comprehend the defining features of stem cells at the system level.…”
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Unravelling 2-oxoglutarate turnover and substrate oxidation dynamics in 5-methylcytosine-oxidising TET enzymes by Šimelis, K, Belle, R, Kawamura, A

“…Here, we profile 5-methylcytosine (5mC)-oxidising Ten-Eleven Translocation (TET) enzymes using MS and 1H NMR spectroscopy methods and reveal a high degree of substrate oxidation-independent 2OG turnover under a range of conditions. 2OG decarboxylase activity is substantial (>20% 2OG turned over after 1 h) in the absence of substrate, while, under substrate-saturating conditions, half of total 2OG consumption is uncoupled from substrate oxidation. 2OG kinetics are affected by substrate and non-substrate DNA oligomers, and the sequence-agnostic effects are observed in amoeboflagellate Naegleria gruberi NgTet1 and human TET2. …”

The synthesis and biological characterisation of high affinity, selective macrocyclic ligands targeting the CREBBP/p300 bromodomains by Boyd, AM

“…These large multi-domain proteins play a key role in gene expression through their recruitment of transcription factors onto DNA, both of these paralogues contain bromodomains. …”

A deep learning system accurately classifies primary and metastatic cancers using passenger mutation patterns by Jiao, W, Atwal, G, Polak, P, Karlic, R, Cuppen, E, PCAWG Tumor Subtypes and Clinical Translation Working Group, Danyi, A, de Ridder, J, van Herpen, C, Lolkema, MP, Steeghs, N, Getz, G, Morris, QD, Stein, LD, PCAWG Consortium

“…Our results have clinical applicability, underscore how patterns of somatic passenger mutations encode the state of the cell of origin, and can inform future strategies to detect the source of circulating tumour DNA.…”

Optimisation strategies for directed evolution without sequencing by James, J, Towers, S, Foerster, J, Steel, H

“…Many of these methods, however, require DNA sequencing and synthesis, making them resource-intensive and incompatible with developments in targeted in vivo mutagenesis. …”

Convergence issues in online marketing models : case study of catalog retailers. by Gupta, Ajay., Ghante, Anantha P. V.

“…The primary objective of this dissertation is to study the on-line retail business models to develop an understanding of the convergence of the catalog retailers in integrating online marketing into their business models.…”
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Studies on the Chitin Binding Property of Novel Cysteine-Rich Peptides from Alternanthera sessilis by Kini, Shruthi G., Nguyen, Phuong Q. T., Weissbach, Sophie, Mallagaray, Alvaro, Shin, Joon, Yoon, Ho Sup, Tam, James P.

“…Hevein-like peptides make up a family of cysteine-rich peptides (CRPs) and play a role in plants in their defense against insects and fungal pathogens. …”
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