Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site by Banach, Bailey B, Pletnev, Sergei, Olia, Adam S, Xu, Kai, Zhang, Baoshan, Rawi, Reda, Bylund, Tatsiana, Doria-Rose, Nicole A, Nguyen, Thuy Duong, Fahad, Ahmed S, Lee, Myungjin, Lin, Bob C, Liu, Tracy, Louder, Mark K, Madan, Bharat, McKee, Krisha, O’Dell, Sijy, Sastry, Mallika, Schön, Arne, Bui, Natalie, Shen, Chen-Hsiang, Wolfe, Jacy R, Chuang, Gwo-Yu, Mascola, John R, Kwong, Peter D, DeKosky, Brandon J

“…Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. …”
Get full text

Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. …”
Get full text
Get full text

Finding transcription factor binding motifs for coregulated genes by combining sequence overrepresentation with cross-species conservation by Jia, Hui., Li, Jinming.

“…Compared with the prevailing motif finding software, our method has some advantages in finding transcriptional factor binding motifs for potential coregulated genes if the gene upstream sequences of multiple closely related species are available. Although we used yeast genomes to assess our method in this study, it might also be applied to other organisms if suitable related species are available and the upstream sequences of coregulated genes can be obtained for the multiple closely related species.…”
Get full text
Get full text

Niemann-Pick type c disease: pathogenesis and therapy by Colaco, A

“…To gain further insight into the function of NPC1 and proteins it interacts with we used the yeast orthologue, Ncr1p. I recreated the Δncr1 mutant and characterized the mutant using an array of systematic screens to identify different processes and pathways that may play a role in NPC pathogenesis. …”

Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods by Mohd Yusof, Rokiah, Haque, Formuzul, Ismail, Maznah, Hassan, Zaiton

“…Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. …”

Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants by Eskandari, Azadeh, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. …”

Unveiling the facade of blog posts to reveal subjective well-being : an exploratory study. by Ou, Zhiqi., Tan, Justine Yi Ting., Tan, Jessie Zi Xi.

“…With the increased usage of social media over the past few years, online platforms like blogs and micro blogs may offer a valuable source of information with regards to behavioral economics and policy design. …”
Get full text

Impact of media literacy on soft drink consumption among adolescents in Klang Valley, Malaysia by Mohammad Shahid, Nur Nadirah

“…Results revealed that 36% of respondents drank a minimum of 1 can, bottle or glass of soft drink for the past seven days and possessed good level of media literacy (45.1%). …”
Get full text

One Plus One is More than Two: A Practical Combination of Power and Fault Analysis Attacks on PRESENT and PRESENT-Like Block Ciphers by Patranabis, Sikhar, Mukhopadhyay, Debdeep, Breier, Jakub, Bhasin, Shivam

“…We present the first practically realizable sidechannel assisted fault attack on PRESENT, that can retrieve the last round key efficiently using single nibble faults. …”
Get full text
Get full text

Putting distance through cleansing : the mediating role of temporal distancing on the embodiment of moral purity by Nurul Mira Fatin Abdulkahar

“…More specifically, it is hypothesized that compared to individuals who do not clean their hands, those who do so after recalling a past immoral act would be more likely to temporally distance their present selves away from their past immoral selves so as to preserve favorable self-view, and hence are more likely to perceive their past moral transgressions as less severe than they would have normally believed. …”
Get full text

Selective load shedding priority algorithm development by Ong, Jing Xian

“…In modern power systems, load shedding is usually the last resort to maintain system stability. Raising the power generated is the go-to method to tackle increasing load demand. …”
Get full text

Nonlinear partial differential equations in neuroscience: from modelling to mathematical theory by Carrillo de la Plata, JA, Roux, P

“…The second part concerns hyperbolic transport equations, namely the model of the Time Elapsed since the last discharge and the jump-based Leaky Integrate and Fire model. …”

Contraceptive experience and factors associated with desire for postpartum family planning among pregnant women of the nkongsamba health district, Littoral Region, Cameroon by Ajong, AB, Yakum, MN, Mangala, FN, Bekolo, CE, Agbor, VN, Waffo, LM, Kenfack, B

“…This study aimed to evaluate past contraceptive experience and identify factors associated with the desire for postpartum family planning among women in late pregnancy. …”

Modeling downward counterfactual events : unrealized disasters and why they matter by Lin, Yolanda C., Jenkins, Susanna F., Chow, Jun Rui, Biass, Sébastien, Woo, Gordon, Lallemant, David

“…Disaster risk research’s reliance on past events has proved inadequate when it comes to extreme events. …”
Get full text

History education for transitional justice: how students understand and construct historical legacies in the post-apartheid South African history classroom by Robinson, N

“…The South African history curriculum – a focus of this study – deals extensively with large-scale past abuses, but does not require students to reflect on whether these past abuses have lasting effects.…”

New York Stock Exchange (NYSE) : Day-of-the-week effect and bid-ask spreads by Guo, Lisa Xiuhui, Ernest Julian Sutanto, Quek, Benny Shiwei

“…We find that the technology sector is associated with higher transaction cost. Last but not least, our results in the last section present the absence of DOW effect in all three types of classifications by trading volume.…”
Get full text

The role of Holocene relative sea-level change in preserving records of subduction zone earthquakes by Dura, Tina, Engelhart, Simon E., Vacchi, Matteo, Horton, Benjamin Peter, Kopp, Robert E., Peltier, William Richard, Bradley, Sarah

“…In near-field regions that were located beneath northern hemisphere ice sheets (e.g., western Vancouver Island), RSL fall from a mid-Holocene highstand has limited prehistoric earthquake records to the last 1 ka. In intermediate field regions (e.g., southern Washington and central Oregon), gradual RSL rise over the last ∼7 ka has preserved widespread records of prehistoric earthquakes. …”
Get full text

React based web-application for stock price forecasting with a novel stacking algorithm by Mittal, Madhav

“…The model performances are compared against a threshold performance of the “Last Value” algorithm. The “Last Value” algorithm is a naïve algorithm that assumes zero volatility in the data and thus sets all forecasts to be the value of the last observation. …”
Get full text

Effectiveness of fludrocortisone plus hydrocortisone versus hydrocortisone alone in septic shock: a systematic review and network meta-analysis of randomized controlled trials by Teja, B, Berube, M, Pereira, TV, Law, AC, Schanock, C, Pang, B, Wunsch, H, Walkey, AJ, Bosch, NA

“…The primary outcome was all-cause mortality at last follow-up. Treatment effects are presented as relative risks (RRs) with 95% credible intervals (CrIs). …”

Predicting EPL results with Elo rating, league simulation and machine learning prediction model by Ching, Kai Teng

“…Team strength can be handicapped when the key player is injured or is forced to take an international break in favor of playing for his country. Even past results may be skewed as there are home teams and away teams in football matches and past results sometimes may be skewed towards having a weaker opponent. …”
Get full text