Multidose transient transfection of human embryonic kidney 293 cells modulates recombinant adeno‐associated virus2/5 Rep protein expression and influences the enrichment fraction o... by Srinivasan, Prasanna, Canova, Christopher T, Sha, Sha, Nguyen, Tam NT, Joseph, John, Sangerman, Jose, Maloney, Andrew J, Katsikis, Georgios, Ou, Rui Wen, Hong, Moo Sun, Ng, Jaclyn, Yuan, Arella, Antov, Daniel, Song, Sally, Chen, Wenyu, Neufeld, Caleb, Wolfrum, Jacqueline M, Barone, Paul W, Sinskey, Anthony J, Springs, Stacy L, Braatz, Richard D

“…Analysis of our previously developed mechanistic model for rAAV2/5 production attributed these low fill fractions to a poorly coordinated timeline between capsid synthesis and viral DNA replication and the repression of later phase capsid formation by Rep proteins. Here, we extend the model by quantifying the expression dynamics of total Rep proteins and their influence on the key steps of rAAV2/5 production using a multiple dosing transfection of human embryonic kidney 293 (HEK293) cells. …”
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Rapid taqman-based quantification of chlorophyll d-containing cyanobacteria in the genus acaryochloris by Behrendt, Lars, Nielsen, Jeppe L., Sørensen, Søren J., Larkum, Anthony W. D., Winther, Jakob R., Kühl, Michael

“…The majority of this evidence is based on amplification of the gene coding for the 16S rRNA, and due to the wide geographical distribution of these sequences, a global distribution of Acaryochloris species was suggested. Here, we present a rapid, reliable, and cost-effective TaqMan-based quantitative PCR (qPCR) assay that was developed for the specific detection of Acaryochloris species in complex environmental samples. …”
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Regulation of mRNA abundance by polypyrimidine tract-binding protein-controlled alternate 5′ splice site choice by Hamid, Fursham M., Makeyev, Eugene V.

“…How alternate splice sites are selected by the splicing machinery and how AS is integrated into gene regulation networks remain important questions of eukaryotic biology. Here we report that polypyrimidine tract-binding protein 1 (Ptbp1/PTB/hnRNP-I) controls alternate 5′ and 3′ splice site (5′ss and 3′ss) usage in a large set of mammalian transcripts. …”
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Direct seeding of mass-cultured coral larvae is not an effective option for reef rehabilitation by Edwards, Alasdair J., Guest, James R., Heyward, Andrew J., Villanueva, Ronald D., Baria, Ma. Vanessa, Bollozos, Iris S. F., Golbuu, Yimnang

“…While some studies have shown positive short-term effects on settlement, to date, none have examined the long-term effects of larval seeding for a broadcast-spawning coral. Here, we test whether larval seeding significantly increases coral recruitment rates both in the short (5 wk) and longer (~6 mo to 1 yr) term. …”
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Universality for the largest eigenvalue of sample covariance matrices with general population by Bao, Zhigang, Pan, Guangming, Zhou, Wang

“…This paper is aimed at deriving the universality of the largest eigenvalue of a class of high-dimensional real or complex sample covariance matrices of the form WN = Σ 1 /2 XX* Σ 1/2. Here, X = (xij)M,N is an M x N random matrix with independent entries xij, 1 ≤ i ≤ M, 1 ≤ j ≤ N such that Exij = 0, E|xij|2 = 1/N. …”
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Protein arginine methyltransferase 6 enhances ligand-dependent and -independent activity of estrogen receptor α via distinct mechanisms by Sun, Yang, Chung, Hwa Hwa, Woo, Amanda Rui En, Lin, Valerie C.-L.

“…However, it is unclear how PRMT6 promotes ERα activity. Here we report that PRMT6 specifically interacts with ERα at its ligand-binding domain. …”
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Modulating macrophage phenotype by sustained microRNA delivery improves host‐implant integration by Lin, Junquan, Ibrahim Mohamed, Lin, Po Hen, Shirahama, Hitomi, Milbreta, Ulla, Sieow, Je Lin, Peng, Yanfen, Bugiani, Marianna, Wong, Siew Cheng, Levinson, Howard, Chew, Sing Yian

“…This opens up a potential avenue for improving host‐implant integration. Here, electrospun poly(caprolactone‐co ‐ethyl ethylene phosphate) nanofiber scaffolds are utilized to deliver microRNAs (miRs) to induce macrophage polarization and modulate FBR. …”
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Biofilm formation inhibition and dispersal of multi-species communities containing ammonia-oxidising bacteria by Keshvardoust, Pejhman, Huron, Vanessa A. A., Clemson, Matthew, Constancias, Florentin, Barraud, Nicolas, Rice, Scott A.

“…The experimental model developed here provides evidence that ammonia-oxidising bacteria (AOB) are involved in both the formation and maintenance of multi-species biofilm communities. …”
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Discovery of a new predominant cytosine DNA modification that is linked to gene expression in malaria parasites by Hammam, Elie, Ananda, Guruprasad, Sinha, Ameya, Scheidig-Benatar, Christine, Bohec, Mylene, Preiser, Peter Rainer, Dedon, Peter C., Scherf, Artur, Vembar, Shruthi S.

“…In the human malaria parasite Plasmodium falciparum, a unicellular eukaryotic pathogen, little is known about the predominant cytosine modifications, cytosine methylation (5mC) and hydroxymethylation (5hmC). Here, we report the first identification of a hydroxymethylcytosine-like (5hmC-like) modification in P. falciparum asexual blood stages using a suite of biochemical methods. …”
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Critical role for cold shock protein YB-1 in cytokinesis by Mehta, Sunali, Algie, Michael, Al-Jabry, Tariq, McKinney, Cushla, Kannan, Srinivasaraghavan, Verma, Chandra Shekhar, Ma, Weini, Zhang, Jessie, Bartolec, Tara K., Masamsetti, V. Pragathi, Parker, Kim, Henderson, Luke, Gould, Maree L., Bhatia, Puja, Harfoot, Rhodri, Chircop, Megan, Kleffmann, Torsten, Cohen, Scott B., Woolley, Adele G., Cesare, Anthony J., Braithwaite, Antony

“…However, the precise mechanism by which YB-1 regulates proliferation is unknown. Here, we found that YB-1 depletion in several cancer cell lines and in immortalized fibroblasts resulted in cytokinesis failure and consequent multinucleation. …”
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Doping in perovskite nanocrystals, perovskite solar cells with organic carrier transport layers, and Selenium solar cells by Liu, Wenbo

“…PSCs can serve as a very good platform to try organic carrier transport materials and promote the development of organic semiconductors. Here, a series of small molecule organic materials with varied functional side chains have been applied as the electron transport layers (ETL) for perovskite materials. …”
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Anion texturing towards dendrite-free Zn anode for aqueous rechargeable battery by Yuan, Du, Zhao, Jin, Ren, Hao, Chen, Yingqian, Chua, Rodney, Tang, Ernest Jun Jie, Cai, Yi, Edison, Eldho, Manalastas, William, Jr., Wong, Ming Wah, Srinivasan, Madhavi

“…Though being widely employed in aqueous energy storage systems, metallic zinc suffers from dendrite formation that severely hinders its applications. Here we report texturing Zn as an effective way to address the issue of zinc dendrite. …”
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"Meta-atomless" architecture based on an irregular continuous fabric of coupling-tuned identical nanopillars enables highly efficient and achromatic metasurfaces by Yaǧcl, H. Bilge, Demir, Hilmi Volkan

“…Although the use of near-field coupling for tuning electromagnetic resonances found its use in constructing efficient narrow-band designs, such structures fell short of providing high efficiency over a broad spectrum. Here, we propose and show that tightly packed fabrics of identical dielectric nanopillar waveguides with continuously tuned intercoupling distances make excellent and complete achromatic metasurface elements. …”
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Remarkably distinct mechanical flexibility in three structurally similar semiconducting organic crystals studied by nanoindentation and molecular dynamics by Devarapalli, Ramesh, Kadambi, Sourabh Bhagwan, Chen, Chun-Teh, Krishna, Gamidi Rama, Kammari, Bal Raju, Buehler, Markus J., Ramamurty, Upadrasta, Reddy, C. Malla

“…Distinct macroscopic mechanical responses of the three crystals of naphthalene diimide derivatives, 1Me, 1Et, and 1nPr, studied here are very intriguing because their molecular structures are very similar, with the difference only in the alkyl chain length. …”
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Biotoxoid photonic sensors with temperature insensitivity using a cascade of ring resonator and Mach–Zehnder interferometer by Li, Zhenyu, Zou, Jun, Zhu, Huihui, Nguyen, Binh Thi Thanh, Shi, Yuzhi, Liu, Patricia Yang, Bailey, Ryan C., Zhou, Jin, Wang, Hong, Yang, Zhenchuan, Jin, Yufeng, Yap, Peng Huat, Cai, Hong, Hao, Yilong, Liu, Ai Qun

“…Additional complex and costly chemical signal enhancement strategies are usually required to improve the signal-to-noise ratio (SNR). Here, a biotoxoid photonic sensor that is resistant to temperature fluctuation have been demonstrated. …”
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Electrically and thermally tunable smooth silicon metasurfaces for broadband terahertz antireflection by Ding, Lu, Luo, Xianshu, Cheng, Liang, Thway, Maung, Song, Junfeng, Chua, Soo Jin, Chia, Elbert E. M., Teng, Jinghua

“…Further advancement of terahertz systems demands efficient and versatile tunable and reconfigurable metadevices for manipulating various properties of terahertz radiation. Here an electrically and thermally tunable silicon metasurface for broadband terahertz antireflection applica- tion is demonstrated. …”
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MOF-based hierarchical structures for solar-thermal clean water production by Ma, Qinglang, Yin, Pengfei, Zhao, Meiting, Luo, Zhiyong, Huang, Ying, He, Qiyuan, Yu, Yifu, Liu, Zhengqing, Hu, Zhaoning, Chen, Bo, Zhang, Hua

“…However, solar water evaporators that exhibit both high water vapor generation ability and anti-oil-fouling ability have not been reported. Here, a unique metal-organic-framework-based hierarchical structure, referred to as MOF-based hierarchical structure (MHS), is rationally designed and prepared, which simultaneously displays a high solar absorption and a superhydrophilic and underwater superoleophobic surface property. …”
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Speeding up deep neural network training with decoupled and analytic learning by Zhuang, Huiping

“…Existing methods have several limitations, such as structural constraints or requesting invertible activation functions. Here a correlation projection network (CPNet) is developed which removes the aforementioned limitations by treating the network as a combination of multiple 2-layer modules. …”
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Observation of an acoustic octupole topological insulator by Xue, Haoran, Ge, Yong, Sun, Hong-Xiang, Wang, Qiang, Jia, Ding, Guan, Yi-Jun, Yuan, Shou-Qi, Chong, Yidong, Zhang, Baile

“…Recent theories have generalised the concept of quantised dipoles to multipoles, resulting in the discovery of multipole topological insulators which exhibit a hierarchy of multipole topology: a quantised octupole moment in a three-dimensional bulk induces quantised quadrupole moments on its two-dimensional surfaces, which in turn induce quantised dipole moments on one-dimensional hinges. Here, we report on the realisation of an octupole topological insulator in a three-dimensional acoustic metamaterial. …”
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Periodontitis associates with species-specific gene expression of the oral microbiota by Belstrøm, Daniel, Constancias, Florentin, Drautz-Moses, Daniela Isabel, Schuster, Stephan Christoph, Veleba, Mark, Mahé, Frédéric, Givskov, Michael

“…Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. …”
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