Characterization of immune response and duration of protection in buffaloes immunized with haemorrhagic septicaemia vaccines by Chandrasekaran, S., Kennett, L., Yeap, P.C., Muniandy, N., Rani, B., Mukkur, T.K.S.

“…These results suggested that there was a relationship between ELISA antibody titres and active protection in buffaloes. …”
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Mechanism of damnacanthal induced apoptosis in CEM-SS cell line by Gopalsamy, Banulata, Saiful Yazan, Latifah, Abdul Hamid, Hisyam

“…Cell viability and morphology was tested with trypan blue assay, flow cytometry analysis detected the apoptotic activity of damnacanthal, caspase colorimetric protease assay tested the Caspase 2, 3, 6, 8, and 9’s involvement and Enzyme-linked Immunosorbent Assay (ELISA) was carried out to quantify the Human p53, Bcl-2, and Bax expression levels. …”
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Linking dual mode of action of host defense antimicrobial peptide thanatin: structures, lipopolysaccharide and LptAm binding of designed analogs by Sinha, Sheetal, Dhanabal, Vidhya Bharathi, Sperandeo, Paola, Polissi, Alessandra, Bhattacharjya, Surajit

“…Further, atomic resolution structures and interactions of Ala based thanatin analogs define plausible correlations with antibacterial activity and LPS, LptAm interactions. …”
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Development and application of novel proteomics methods in proteomics research by Hao, Piliang

“…The existing challenges for accurately identifying and quantifying protein deamidation sites in proteomics include tremendous false positive identifications of deamidated peptides from database searches, substantial interference of artificial deamidation from sample preparation, difficulties in differentiating and quantifying the isomeric n-Asp and isoAsp deamidation products in proteome scale, and inability of validating quantification of deamidated peptides by either ELISA or multiple reaction monitoring. In this thesis, novel proteomic methods were developed to overcome these challenges and facilitate the large-scale characterization of protein deamidation. …”
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Are all renewable energy sources the same? a comparative analysis of public perceptions and preferences for renewable energy types in Southeast Asian cities by del Barrio Alvarez, Daniel, Wang, Nan, Sugiyama, Masahiro, Numata, Masako, Thuy, Truong Dang, Abd Aziz, Azlina, Palanca-Tan, Rosalina, Ze Ya, Aung, Yusuf, Ariel Anshory, Abd. Ghani, Awang Noor, Castillo, Gem, Siyaranamual, Martin Daniel

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Cross-reactive dengue human monoclonal antibody prevents severe pathologies and death from Zika virus infections by Kam, Yiu-Wing, Lee, Cheryl Yi-Pin, Teo, Teck-Hui, Howland, Shanshan W., Siti Naqiah Amrun, Lum, Fok-Moon, See, Peter, Kng, Nicholas Qing-Rong, Huber, Roland G., Xu, Mei-Hui, Tan, Heng-Liang, Choo, Andre, Maurer-Stroh, Sebastian, Ginhoux, Florent, Fink, Katja, Wang, Cheng-I, Ng, Lisa F.P., Rénia, Laurent

“…The SIgN-3C variant with Leu-to-Ala mutations in the Fc region (SIgN-3C-LALA) did not induce antibody-dependent enhancement (ADE) in vitro but provided similar levels of protection in vivo. …”
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Airway proteomics reveals broad residual anti-inflammatory effects of prednisolone in mepolizumab-treated asthma by Howell, I, Yang, F, Brown, V, Cane, J, Marchi, E, Azim, A, Busby, J, McDowell, PJ, Diver, SE, Borg, C, Heaney, LG, Pavord, ID, Brightling, CE, Chaudhuri, R, Hinks, TSC

“…We also analysed additional sputum proteins using ELISA. <p><b>Results</b></p> In patients receiving mepolizumab, prednisolone significantly downregulated sputum proteins related to type-2 inflammation and chemotaxis including IL-4, IL-5, IL-13, CCL24, CCL26, EDN, CCL17, CCL22, OX40 receptor, FCER2, and the ST2 receptor. …”

Immunological screening of Lassa virus among health workers and contacts of patients of Lassa fever in Ondo State by Shaibu, JO, Salu, OB, Amoo, OS, Idigbe, I, Musa, AZ, Ezechi, OC, Abejegah, C, Ayodeji, O, Salako, BL, Omilabu, SA, Audu, RA

“…Plasma samples were analyzed for IgG and IgM markers using ReLASV® Pan-Lassa NP IgG/IgM ELISA Kit (Zalgen Labs, LLC, USA) while RNAs extracted from IgM positive samples were analyzed for LASV RNA according to manufacturers’ instructions. …”

Inhibition of nitric oxide production in RAW 264.7 cells and cytokines IL-1β in osteoarthritis rat models of 70 % ethanol extract of Arcangelisia flava (L.) merr stems by Pratama, Rizki Rahmadi, Sari, Riza Ambar, Sholikhah, Irawati, Mansor, Hakiman, Chang, Hsin-I, Sukardiman, Widyowati, Retno

“…The NO inhibition test was determined by the NO colorimetric assay using Griess reagent and measured by the ELISA plate reader. The measurement of joint diameter and hyperalgesia in osteoarthritis rats was carried out once a week for 7 weeks, and then the IL-1β levels were measured at weeks 3 and 7. …”
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Intraperitoneal injection of the Porphyromonas gingivalis outer membrane vesicle (OMV) stimulated expressions of neuroinflammatory markers and histopathological changes in the brai... by Adewoyin, Malik, Hamarsha, Ahmed, Akinsola, Rasaq, Teoh, Seong Lin, Azmai, Mohammad Noor Amal, Abu Bakar, Noraini, Nasruddin, Nurrul Shaqinah

“…Brains of experimental zebrafish were dissected at desired time points for colorimetric assays, ELISA, and histology. This study discovered that nitric oxide and PGE2 were significantly increased at 45 min, while IL-1β and IL-6 were expressed at subsequent 12 h and 24 h time points, respectively. …”
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Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma by Lyu, Zhenlei, Mahenderan, Appukutty, Radhakrishnan, Ammu Kutty G. K., Chin, Yit Siew, Yin, Chao

“…CCK-8 and colony formation assays were used to detect cell viability and proliferation. ELISA was employed to determine the levels of cytokines secreted by macrophages. …”
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Assessment of phagocytosis and cytokine secretions by monocytes in the presence of plasmodium falciparum by Xuan, Keh Min

“…falciparum, to assess phagocytic capability of infected red blood cells by human monocytes and further measure the cytokine secretions of monocytes following phagocytosis by using ELISA. In this study, monocytes were isolated from whole blood collected from healthy individuals while Plasmodium falciparum (3D7) was cultured under optimal conditions. …”
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Effects of transport inhibitors on the cellular uptake of liposomes from mycobacterium smegmatis by human monocytes dendritic cells by Nordin, Nor Asyikin

“…Supernatant will be collected to measure the cytokine level by ELISA. Therefore, in this proposed study, we are going to fill up the gaps by investigating the uptake mechanisms of liposomes-Msmeg as a potent lipid-based vaccine by DCs from PBMC in healthy individuals.…”
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Towards a Single Bio-molecule Detector Based on CMOS Nanofluidic Platform by Zikrallah, Ahmed S.

“…Ideally, we would be able to continuously monitor the secretome of these cell therapies as they are transformed and expanded in manufacturing.However, state-of-theart techniques for monitoring typically low concentrations of cytokines require either Mass Spectroscopy (MS) or immunoassays like Enzyme-linked Immunosorbent Assay (ELISA). We propose the use of CMOS technology to build a proteomic platform with a single biomolecule resolution. …”
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Skin dendritic cell and T cell activation associated with dengue shock syndrome by Duyen, Huynh Thi Le, Cerny, Daniela, Trung, Dinh The, Pang, Jassia, Velumani, Sumathy, Toh, Ying Xiu, Qui, Phan Tu, Hao, Nguyen Van, Simmons, Cameron, Haniffa, Muzlifah, Wills, Bridget, Fink, Katja

“…We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. …”
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The chronic cerebral hypoperfusion model induces proinflammatory cascades in Alzheimer's disease by Abedi, Zahra, Basri, Hamidon, Hassan, Zurina, Inche Mat, Liyana Najwa, Khaza’ai, Huzwah, Mohd Ali, Razana

“…The gene expression levels of cathepsin B, NLRP3, an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 were analysed using real-time PCR, while the expression levels of the inflammatory cytokines were estimated using the ELISA method. Structural damage to the hippocampus was assessed using hematoxylin and eosin (HE) staining. …”
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Micro electro mechanical systems (MEMS) enabled digital pen by Tan, Joel Qi Zhi

“…Upon completing the data collection, data were imported into AlaVar5 to compute the Allan standard deviation of each axis of the accelerometer and gyroscope. …”
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Nuclear magnetic resonance spectroscopic studies of the structure and interactions between hepatitis B virus core and surface antigens with peptides by Muhamad, Azira

“…In a previous study, a disulfide constrained cyclic peptide cyclo S1,S9 Cys-Glu-Thr-Gly-Ala-Lys-Pro-His-Cys (S1, S9-cyclo-CETGAKPHC) was isolated from a phage displayed cyclic peptide library using an affinity selection method against HBsAg. …”
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Seroepidemiology of COVID-19 in pregnant women and their infants in Uganda and Malawi across multiple waves 2020-2022 by Hookham, L, Cantrell, L, Cose, S, Freyne, B, Gadama, L, Imede, E, Kawaza, K, Lissauer, S, Musoke, P, Nankabirwa, V, Sekikubo, M, Sommerfelt, H, Voysey, M, PeriCOVID Consortium, Le Doare, K

“…A nasopharyngeal swab was taken from mothers at enrolment for RT-PCR confirmation of SARS-CoV-2 infection, and maternal and cord blood samples were tested for SARS-CoV-2 antibodies using Wantai and Euroimmune ELISA. The primary outcome was seroprevalence of SARS-CoV-2 antibodies in maternal blood, reported as the proportion of seropositive women by study site and wave of COVID-19 within each country. …”

Effects of ifenprodil treatment on pain behaviour, inflammation, and nociceptive responses in the spinal cord of complete freund’s adjuvant-induced chronic polyarthritis rat by Noh, Ain’ Sabreena Mohd

“…Histopathological examination was performed on the ipsilateral ankle joint while the lumbar region of the spinal cord (L4-L5) was collected for ELISA, immunohistochemistry and RT-qPCR analyses. …”
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