Biomolecular hydration in protein-protein interaction, protein stability and aggregation and lead optimization : computational studies by Mohan Rajan Pradhan

“…Driven by the observation that restoring the wild type function of mutant p53 is known to prevent tumors, I am in the process of screening libraries of compounds against this new pocket to identify binders that may guide the development of molecules to restabilize mutant p53. …”
Get full text

Phosphatidylinositol 4,5-Bisphosphate Is an HCV NS5A Ligand and Mediates Replication of the Viral Genome by Cho, Nam-Joon, Lee, Choongho, Pang, Phillip S., Pham, Edward A., Fram, Benjamin, Nguyen, Khanh, Xiong, Anming, Sklan, Ella H., Elazar, Menashe, Koytak, Elif S., Kersten, Caroline, Kanazawa, Kay K., Frank, Curtis W., Glenn, Jeffrey S.

“…Methods: We used a combination of quartz crystal microbalance, circular dichroism, molecular genetics, and immunofluorescence to study specific binding of PI(4,5)P2 by the HCV NS5A protein. We evaluated the effects of PI(4,5)P2 on the function of NS5A by expressing wild-type or mutant forms of Bart79I or FL-J6/JFH-5’C19Rluc2AUbi21 RNA in Huh7 cells. …”
Get full text
Get full text

Elucidating the mechanism of mRNA transport using novel imaging technologies by Stoychev, D

“…The MS2-MCP system is used to insert into mRNAs of interest special structures to which RNA-binding proteins, fused to a fluorescent protein such as GFP, can then bind. …”

The molecular pathogenesis of FUS mutations in amyotrophic lateral sclerosis using in vivo and in vitro models by Kent, L

“…Fused in sarcoma (FUS) is an RNA binding protein, and mutations in the FUS gene are responsible for approximately 4% of familial and <1% of sporadic ALS cases. …”

Methods for the Study of Galactofuranose in Mycobacteria by Taylor, Katherine I.

“…Finally, we characterized the kinetics of a mild electrophile, the squaric ester, and assessed its utility in selectively binding and modifying a key galactofuranosyl transferase involved in mycobacterial cell wall biosynthesis. …”
Get full text

Vypal2: a versatile peptide ligase for precision tailoring of proteins by Zhang, Dingpeng, Wang, Zhen, Hu, Side, Lescar, Julien, Tam, James P., Liu, Chuan-Fa

“…The last two decades have seen an increasing demand for new protein-modification methods from the biotech industry and biomedical research communities. Owing to their mild aqueous reaction conditions, enzymatic methods based on the use of peptide ligases are particularly desirable. …”
Get full text

Seroprevalence of SARS-CoV-2 in children and adolescents by Ratcliffe, H

“…</p> <br> <p>The findings of ‘STORY’ confirm that SARS-CoV-2 infections in children are generally asymptomatic or mild and rarely progress to severe disease and hospitalisation. …”

Methyl and dimethyl o-xylenyl-substituted fullerene acceptors for polymer solar cells by Kim, Hee Un, Park, Jong Baek, Grimsdale, Andrew Clive, Hwang, Do-Hoon

“…These values correlate with the binding affinities of the fluorophore and quencher. …”
Get full text
Get full text

Selectivity of stop codon recognition in translation termination is modulated by multiple conformations of GTS loop in eRF1 by Li, Yan, Pillay, Shubhadra, Frolova, Ludmila, Pervushin, Konstantin, Wong, Leo E.

“…By solving the solution structures of the wild-type N-domain of human eRF1 exhibited omnipotent specificity, i.e. recognition of all three stop codons, and its unipotent mutant with UGA-only specificity, we found the conserved GTS loop adopting alternate conformations. …”
Get full text
Get full text

Using an ER-specific optogenetic mechanostimulator to understand the mechanosensitivity of the endoplasmic reticulum by Song, Yutong, Zhao, Zhihao, Xu, Linyu, Huang, Peiyuan, Gao, Jiayang, Li, Jingxuan, Wang, Xuejie, Zhou, Yiren, Wang, Jinhui, Zhao, Wenting, Wang, Likun, Zheng, Chaogu, Gao, Bo, Jiang, Liwen, Liu, Kai, Guo, Yusong, Yao, Xiaoqiang, Duan, Liting

“…Sustained mechanostimuli increase the levels of binding-immunoglobulin protein (BiP) expression and phosphorylated eIF2α, two markers for ER stress. …”
Get full text

Investigating the role of CDK1 in governing the transcriptional landscape in cancer cells by Pluta, AJ

“…Subsequent analysis of the upregulated genes identified several transcription factors as candidates for involvement in mediating the transcriptional effects of CDK1 across different cell types, after their binding motifs were found to be enriched upstream of the transcription start site of the dysregulated genes. …”

Crack healing in reactive MgO-slag system via bacteria induced carbonate precipitation by Zheng, Yanying

“…In the Concrete industry, the most widely used binding ingredient for concrete, portland cement (PC), is accountable for around 8% of global anthropogenic carbon dioxide (CO2) emissions. …”
Get full text

Conformational changes involved in signal transduction in avian cryptochromes by Gehrckens, AS

“…</p> <p>Finally, by mapping conformational changes found in molecular dynamics simulations onto changes observed in a previous HDX-MS data set on the <em>Drosophila</em> cryptochrome, a few gaps left by the incomplete coverage of the current experimental data were filled by acquiring information on the phosphate-binding loop and the protrusion motif, two regions which have also been found to undergo light-induced structural changes for the avian cryptochromes investigated. …”

Three-component Friedel-Crafts-type difunctionalization of ynamides with (hetero)arenes and iodine(iii) electrophile by Kikuchi, Jun, Nagata, Toya, Ito, Shingo, Yoshikai, Naohiko

Get full text

Theoretical Modelling and Facile Synthesis of a Highly Active Boron-Doped Palladium Catalyst for the Oxygen Reduction Reaction by Vo Doan, Tat Thang, Wang, Jingbo, Poon, Kee Chun, Tan, Desmond Chun Long, Khezri, Bahareh, Webster, Richard David, Su, Haibin, Sato, Hirotaka

“…Our theoretical modelling reveals that B-doped Pd (Pd-B) weakens the absorption of ORR intermediates with nearly optimal binding energy by lowering the barrier associated with O2 dissociation, suggesting Pd-B should be highly active for ORR. …”
Get full text
Get full text

Translational GTPase BipA is involved in the maturation of a large subunit of bacterial ribosome at suboptimal temperature by Goh, Kwok Jian, Ero, Rya, Yan, Xin-Fu, Park, Jung Eun, Kundukad, Binu, Zheng, Jun, Sze, Siu Kwan, Gao, Yong-Gui

“…Furthermore, tandem mass tag-mass spectrometry analysis of the ΔbipA strain proteome revealed upregulations of a number of proteins (e.g., DeaD, RNase R, CspA, RpoS, and ObgE) implicated in ribosome biogenesis and RNA metabolism, and these proteins were restored to wild-type levels by plasmid-borne bipA supplementation or the genomic rluC deletion, implying BipA involvement in RNA metabolism and ribosome biogenesis. …”
Get full text

Optical properties of semi-conductive MXene Sc2COx, CsPbBr3 nanoplatelets and quantum dots by Chen, Qiran

“…For any given NPL with a certain thickness, there is a slight blue shift in the peak position of optical gain as the carrier density increases because of band filling effect. The maximum optical gain of thinner NPLs needs higher carrier density to reach saturation. …”
Get full text