A model for cis-regulation of transcriptional condensates and gene expression by proximal lncRNAs by Natarajan, Pradeep, Shrinivas, Krishna, Chakraborty, Arup K

“…Long noncoding RNAs (lncRNAs) perform several important functions in cells including cis-regulation of transcription. …”
Get full text

Live-cell imaging and functional dissection of Xist RNA reveal mechanisms of X chromosome inactivation and reactivation by Ha, Norbert How Ong, Lai, Lan-Tian, Chelliah, Rosi, Zhen, Yashu, Seet, Vanessa Pei Yi, Lai, Soak-Kuan, Li, Hoi-Yeung, Ludwig, Alexander, Sandin, Sara, Chen, Lingyi, Zhang, Li-Feng

“…We double-tagged Xist (inactivated X chromosome-specific transcript), a prototype long non-coding RNA pivotal for X chromosome inactivation (XCI), using the programmable RNA sequence binding domain of Pumilio protein, one tag for live-cell imaging and the other replacing A-repeat (a critical domain of Xist) to generate “ΔA mutant” and to tether effector proteins for dissecting Xist functionality. …”
Get full text
Get full text

Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2 by Gu, Yue, Shunmuganathan, Bhuvaneshwari, Qian, Xinlei, Gupta, Rashi, Tan, Rebecca S. W., Kozma, Mary, Purushotorman, Kiren, Murali, Tanusya M., Tan, Nikki Y. J., Preiser, Peter Rainer, Lescar, Julien, Nasir, Haziq, Somani, Jyoti, Tambyah, Paul A., Smith, Kenneth G. C., Renia, Laurent, Ng, Lisa F. P., Lye, David C., Young, Barnaby E., MacAry, Paul A.

Get full text

Investigating viral glycosylation pathways for drug and vaccine development by Brun, J

“…Previously, it had been observed that a single high-dose treatment with the iminosugar MON-DNJ led to long-lasting antiviral effects in lethal dengue mouse models via alpha-glucosidase I inhibition. …”

Nonreciprocal phenomena in superconductivity by Davydova, Marharyta

“…Nonreciprocal superconductors describe certain classes of unconventional superconductors that include certain kinds of mixed-pairing and finite-momentum ones. …”
Get full text

Outer-membrane permeabilization, LPS transport inhibition: activity, interactions, and structures of thanatin derived antimicrobial peptides by Swaleeha Jaan Abdullah, Yan, Bernice Tan Siu, Palanivelu, Nithya, Dhanabal, Vidhya Bharathi, Bifani, Juan Pablo, Bhattacharjya, Surajit

“…Thanatin, a 21-residue-long insect-derived antimicrobial peptide (AMP), is a promising lead molecule for the potential development of novel antibiotics. …”
Get full text

Structure of the C-terminal domain of the multifunctional ICP27 protein from herpes simplex virus 1 by Nordlund, Par, Patel, Vidhi, Dahlroth, Sue-Li, Rajakannan, Venkatachalam, Ho, Hai Ting, Cornvik, Tobias

“…The structure reveals the C-terminal half of ICP27 to have a novel fold consisting of -helices and long loops, along with a unique CHCC-type of zinc-binding motif. …”
Get full text
Get full text

Gene expression regulation by well-known non-coding RNAs by Tan, Jiazi

“…Our results extend our knowledge on the flexibility of the 5’ss/U1 RNA duplex structure that leads to productive splicing. The Xist long ncRNA is essential for random X chromosome inactivation (XCI). …”
Get full text

Identification of bacterial biofilm and the Staphylococcus aureus derived protease, staphopain, on the skin surface of patients with atopic dermatitis by Sonesson, Andreas, Przybyszewska, Kornelia, Eriksson, Sigrid, Mörgelin, Matthias, Kjellström, Sven, Davies, Julia, Potempa, Jan, Schmidtchen, Artur

Get full text
Get full text

Biomolecular hydration in protein-protein interaction, protein stability and aggregation and lead optimization : computational studies by Mohan Rajan Pradhan

“…After providing a brief overview of biomolecular hydration and outlining the need for new insights to explain experimental observations such as aggregation (Chapter 1), a hydration metric is identified that is linked to Aggregation Prone Regions (APRs) in proteins. …”
Get full text

Development of high-affinity peptide-drug conjugates for investigating the TRIM24 PHD and bromodomain by Platt, MA

“…</p> <br> <p>The PDCs showed low nanomolar^® IC₅₀ values for displacement of a TRIM24 PHD- and BRD-binding peptide (H3K9Me₃K18Ac), and exhibited avidity for TRIM24, which arises from simultaneous in <em>cis</em> binding to the PHD and BRD. …”

Imprinting of IgA responses in previously infected individuals receiving bivalent mRNA vaccines (WT and BA.4/BA.5 or WT and BA.1) by Goh, Yun Shan, Fong, Siew-Wai, Hor, Pei Xiang, Loh, Chiew Yee, Tay, Matthew Zirui, Wang, Bei, Salleh, Siti Nazihah Mohd, Ngoh, Eve Zi Xian, Lee, Raphael Tze Chuen, Poh, Xuan Ying, Lee, I. Russel, Rao, Suma, Chia, Po Ying, Maurer-Stroh, Sebastian, Wang, Cheng-I, Leo, Yee Sin, Lye, David C., Young, Barnaby Edward, Ng, Lisa F. P., Renia, Laurent

“…Studies are needed to understand how to induce a robust and long-lasting IgA immunity for protection against COVID-19 infection.…”
Get full text

Synthetic post-translational protein modification as a switch for activity by Faulkner, SC

“…Dha chemistry was once again employed to install O- and N-linked GlcNAc mimics in the complement determining region (CDR) of a nanobody cAbLys3, with the aim of reversibly blocking its antigen binding capacity. …”

Cellular studies on the role of OGFOD1, a 2-oxoglutarate-dependent dioxygenase by Attwood, M

“…However, it was not known whether these functions are conserved in mammalian cells or if they are linked.</p> <p>To inform on OGFOD1-dependent cellular phenotypes, OGFOD1 defective cells were generated by genome editing using the CRISPR methodology. …”

Layer-by-Layer Nanoparticles for Cytokine Delivery by Pires, Ivan S.

“…We also find that the LbL film conformation is key to maintaining therapeutic efficacy as thicker films hinder IL-12 delivery. …”
Get full text

Core level shift of diamond and graphite upon Ar+ ion bombardment by Geng, Han He.

“…Diamond-like films (DLF) and highly oriented pyrolytic graphite (HOPG) are made of two allotropes of carbon, diamond and graphite. …”
Get full text

Functional metagenomic analysis of quorum sensing signaling in a nitrifying community by Tan, Chuan Hao, Yeo, Yee Phan, Muhammad Hafiz Ismail, Ng, Noele Kai Jing, Subramoni, Sujatha, Taj, Shireen, Tay, Martin, Chao, Xie, Kjelleberg, Staffan, Rice, Scott A.

Get full text

Investigation of Synthetic Proteins Produced via Automated Fast-Flow Peptide Synthesis by Cowfer, Amanda Elizabeth

“…With modern research requiring rapid and high-fidelity access to long polypeptide chains, an immediate need to develop peptide synthesis technology to produce single-domain protein polypeptides in a single shot will be critical. …”
Get full text

Three-component Friedel-Crafts-type difunctionalization of ynamides with (hetero)arenes and iodine(iii) electrophile by Kikuchi, Jun, Nagata, Toya, Ito, Shingo, Yoshikai, Naohiko

Get full text

Observation of E-cadherin adherens junction dynamics with metal-induced energy transfer imaging and spectroscopy by Chen, T, Karedla, N, Enderlein, J

“…This knowledge enhances our comprehension of tissue and organ development and may serve as a foundation for the development of innovative therapeutic strategies for diseases linked to cell-cell adhesion abnormalities.…”