Investigation of electrochemistry of vitamin K1-lipid emulsion films deposited on electrode surface in the presence and absence of nafion multilayers. by Poh, Hwee Ling.

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Live-cell imaging and functional dissection of Xist RNA reveal mechanisms of X chromosome inactivation and reactivation by Ha, Norbert How Ong, Lai, Lan-Tian, Chelliah, Rosi, Zhen, Yashu, Seet, Vanessa Pei Yi, Lai, Soak-Kuan, Li, Hoi-Yeung, Ludwig, Alexander, Sandin, Sara, Chen, Lingyi, Zhang, Li-Feng

“…We double-tagged Xist (inactivated X chromosome-specific transcript), a prototype long non-coding RNA pivotal for X chromosome inactivation (XCI), using the programmable RNA sequence binding domain of Pumilio protein, one tag for live-cell imaging and the other replacing A-repeat (a critical domain of Xist) to generate “ΔA mutant” and to tether effector proteins for dissecting Xist functionality. …”
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TCR affinity biases Th cell differentiation by regulating CD25, Eef1e1, and Gbp2 by Kotov, Dmitri I., Mitchell, Jason S., Pengo, Thomas, Ruedl, Christiane, Way, Sing Sing, Langlois, Ryan A., Fife, Brian T., Jenkins, Marc K.

“…Naive CD4+ T lymphocytes differentiate into various Th cell subsets following TCR binding to microbial peptide:MHC class II (p:MHCII) complexes on dendritic cells (DCs). …”
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A yeast two-hybrid system for the screening and characterization of small-molecule inhibitors of protein–protein interactions identifies a novel putative Mdm2-binding site in p53 by Wong, Jin Huei, Alfatah, Mohammad, Sin, Mei Fang, Sim, Hong May, Verma, Chandra Shekhar, Lane, David P., Arumugam, Prakash

“…In addition, we identified a conserved segment in the core DNA-binding domain of p53 that facilitates stable interaction with Mdm2 in yeast cells and in vitro. …”
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HSV-1 amplicon system for human artificial chromosome formation in human ES/iPS cells and pluripotency induction by Khoja, S

“…The HAC system, therefore, needs further refinements to improve the frequency of HAC formation and reduce the chromosomal integration of HAC constructs in hiPS cells. Overall, these findings provide a simple and safe way of pluripotency induction and genetic modification of pluripotent stem cells using the HSV-1 amplicon system and represent an important advance towards patient specific gene and cell therapy.…”

Understanding the molecular mechanism of ion and amino acid transport in neurons by Baroniņa, A

“…To determine the kinetic parameters of ligand binding in vitro, I established a proteoliposome reconstitution system for detergent-purified EAAT3 and showed that the protein is functional and transports substrates with affinities consistent with results from cell-based studies reported in the literature.…”

Endothelial damage arising from high salt hypertension is elucidated by vascular bed systematic profiling by Vinaiphat, Arada, Pazhanchamy, Kalailingam, JebaMercy, Gnanasekaran, Ngan, SoFong Cam, Leow, Melvin Khee-Shing, Ho, Hee Hwa, Gao, Yong-Gui, Lim, Kah-Leong, Richards, A. Mark, de Kleijn, Dominique P. V., Chen, Christopher P., Kalaria, Raj N., Liu, Jian, O'Leary, Deborah D., McCarthy, Neil E., Sze, Siu Kwan

“…Despite extensive epidemiological and basic science interrogation of the relationship between high salt (HS) intake and blood pressure, it remains unclear how HS impacts endothelial cell (EC) and vascular structure in vivo. This study aims to elucidate HS-induced vascular pathology using a differential systemic decellularization in vivo approach. …”
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Optical properties of ultrathin metal oxide and metal films : effect of free electrons, quantum confinement and localized surface plasmon resonance by Li, Xiao Dong

“…The evolution of the dielectric function is shown related to the changes in the interband absorption, discrete-exciton absorption, and continuum-exciton absorption, which can be attributed to the quantum confinement effect on both the band gap and exciton binding energies. Thickness dependence of optical properties of a-IGZO thin films has also been observed. …”
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Methyl and dimethyl o-xylenyl-substituted fullerene acceptors for polymer solar cells by Kim, Hee Un, Park, Jong Baek, Grimsdale, Andrew Clive, Hwang, Do-Hoon

“…These values correlate with the binding affinities of the fluorophore and quencher. …”
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Development of a protein nanoparticle platform for targeting EGFR expressing cancer cells by Buecheler, Jakob W., Howard, Christopher B., de Bakker, Christopher J., Goodall, Stephen, Jones, Martina L., Win, Thinzar, Peng, Tao, Tan, Cher Heng, Chopra, Akhil, Mahler, Stephen M., Lim, Sierin

“…The conjugation of the anti-EGFR antibody fragment (26 kDa) with a subunit of the E2 protein (26 kDa) was confirmed by mass spectrometry with an estimated molecular weight of 52 kDa. The binding of the conjugated E2 particle to native EGFR on MDA MB 231 cells and recombinant EGFR was confirmed using flow cytometry and biolayer interferometry, respectively. …”
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Physicochemical Properties of Films from Semirefined Carrageenan/TiO2 Integrated with Cinnamaldehyde Pickering Emulsion for Active Food Packaging by Khadijah Husna, Abd Hamid, Azilah, Ajit, Azren Aida, Asmawi, Mohd Hafiz, Arzmi, Nurul Aini, Mohd Azman

“…Scanning electron microscopy (SEM) revealed a uniform distribution of active compounds in the SRC matrix. The study findings highlight the potential of cinnamaldehyde PE and TNPs in active food packaging films as eco-friendly alternatives to conventional petrochemical-derived plastics in food packaging.…”
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Metal mediated high performance antimicrobial hydrogel films for wound infection management: Zn, Cu, and Mg versus Ag and Au by Sen, Raj Kumar., Prabhakar, Priyanka, Mayandi, Venkatesh, Dwivedi, Neeraj, Yadav, Amit K., Solanki, Pratima R., Gupta, Ayush, Gowri, V. S., Lakshminarayanan, Rajamani, Verma, Navin Kumar, Mondal, D. P., Srivastava, Avanish Kumar, Dhand, Chetna

“…We present non-traditional yet high-performance hydrogel (HG)-based wound films based on polyvinyl alcohol (PVA), chitosan (CS), polyethylene glycol (PEG), and effective antibacterial metal ions (Zn2+, Cu2+, Ag+, Au3+, and Mg2+) for wound infection management. …”
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Cytoplasmic long noncoding RNAs are frequently bound to and degraded at ribosomes in human cells by Carlevaro-Fita, Joana, Rahim, Anisa, Guigó, Roderic, Johnson, Rory, Vardy, Leah Karen Anne

“…Here we make a global, absolute estimate of the cytoplasmic and ribosome-associated population of stringently filtered lncRNAs in a human cell line using polysome profiling coupled to spike-in normalized microarray analysis. …”
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Heterogeneity within colorectal cancer cell lines and epigenetic regulation of CD24 by Ibn Ayub, M

“…This thesis investigated the role of methylation in dynamic regulation of CD24 based heterogeneity in colorectal cancer cell lines. First, E-Cadherin variation between the cell lines LS174T and LS180 was investigated to find out whether E-Cadherin had any causal role in the difference in lumen formation between these two cell lines derived from the same tumour. …”

The emergence of DNAM-1 as the facilitator of NK cell-mediated killing in ovarian cancer by Pounds, R, Croft, W, Pearce, H, Hossain, T, Singh, K, Balega, J, Jeevan, DN, Sundar, S, Kehoe, S, Yap, J, Moss, P, Zuo, J

“…Discussion: These findings reveal profound modulation of the tumour tissue and systemic profile of NK cells which likely contributes to the high rates of local progression and metastasis seen with OC. …”

Interaction of TiO2 and ZnO NPs with human skin cells by Gautam, Archana

“…Therefore, the goal of this study is to assess the physiological effects of TiO2 and ZnO NPs interactions with primary human epidermal keratinocytes and dermal fibroblasts, with attention to accurate dosimetry measurements and particle solubility considerations. Since the current findings on NPs interaction with skin cells are mostly based on 2D monolayer cell culture and animal models, in this study, 3D human skin explants have also been used to better understand the effects of NPs in the skin tissue. …”
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Effects of free electrons and quantum confinement in ultrathin ZnO films : a comparison between undoped and Al-doped ZnO by Li, X. D., Liu, P., Liu, Y., Leong, K. C., Chen, Tupei

“…Band gaps and exciton binding energies of undoped and Al-doped ZnO thin films were determined from optical absorption measurement based on the Elliott’s exciton absorption theory. …”
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Membrane cholesterol modulates oligomeric status and peptide-membrane interaction of severe acute respiratory syndrome coronavirus fusion peptide by Meher, Geetanjali, Bhattacharjya, Surajit, Chakraborty, Hirak

“…The N-terminal fusion peptide (residues 770-788) of an S2 glycoprotein of the severe acute respiratory syndrome coronavirus (SARS-CoV), exposed upon receptor binding, is crucial for virus entry into the host cell. …”
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Defining proteoform-specific interactions for drug targeting in a native cell signalling environment by Lutomski, CA, Bennett, JL, El-Baba, TJ, Wu, D, Hinkle, JD, Burnap, SA, Liko, I, Mullen, C, Syka, JEP, Struwe, WB, Robinson, CV

“…Understanding the dynamics of membrane protein–ligand interactions within a native lipid bilayer is a major goal for drug discovery. Typically, cell-based assays are used, however, they are often blind to the effects of protein modifications. …”

Visualization of robust two-dimensional bulk states with suppressed surface state on epitaxial PdCoO₂ thin films with bipolar surfaces by Kawamoto, Tomoki, Krishnadas, Anjana, Hsu, Chia-Hsiu, Pardo-Almanza, Markel, Fujisawa, Yuita, Chang, Guoqing, Harada, Takayuki, Okada, Yoshinori

“…Furthermore, the origin of the suppressed surface state can be interpreted by screening of the polarity on the surface, which is qualitatively supported by density functional theory calculation. These findings are invaluable for accelerating the search for exotic functionalities in epitaxial ultrathin films and heterostructures of metallic delafossites.…”
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