Expanding the CRISPR Toolbox for Engineering Lycopene Biosynthesis in Corynebacterium glutamicum by Zhan, Zhimin, Chen, Xiong, Ye, Zhifang, Zhao, Ming, Li, Cheng, Gao, Shipeng, Sinskey, Anthony J., Yao, Lan, Dai, Jun, Jiang, Yiming, Zheng, Xueyun

“…By optimizing the promoter, ORI and PAM sequences, the CRISPR/MAD7 system facilitated highly efficient gene deletion and exhibited a broad spectrum of PAM sites. …”
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Computational Approaches for Understanding and Redesigning Enzyme Catalysis by Karvelis, Elijah

“…These variations in mutant activity on AHB were explained by analysis of WT-AHB simulations, which showed that only some of the structural mechanisms related to enhanced ACL catalysis transferred to, and thereby facilitated, AHB catalysis. This thesis highlights the influence of conformational states that are visited during the dynamics of substrate turnover and their role on enzyme catalysis, and it furthermore suggests a framework with which researchers may consider and apply these effects when engineering catalytic function.…”
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Novel acetamide indirectly targets mycobacterial transporter MmpL3 by proton motive force disruption by Shetty, Annanya, Xu, Zhujun, Lakshmanan, Umayal, Hill, Jeffrey, Choong, Meng Ling, Chng, Shu-Sin, Yamada, Yoshiyuki, Poulsen, Anders, Dick, Thomas, Gengenbacher, Martin

“…Biochemical analysis using mycobacterial spheroplasts and various membrane assays suggest that E11 indirectly inhibits MmpL3-facilitated translocation of trehalose monomycolates by proton motive force disruption. …”
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The peculiarities of knowledge management processes in SMEs : the case of Singapore by Wee, Janet Chui Neo, Chua, Alton Yeow Kuan

“…In SMEs, knowledge creation takes the form of innovative customized solutions to meet customers' needs; knowledge sharing occurs through cross functionality, overlapping roles, and facilitated by close physical proximity in open workspaces; and knowledge reuse is often made tacitly, where common knowledge is prevalently embedded within the KM processes of SMEs. …”
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Cold atoms laboratory in a hollow core fiber by Leong, Wui Seng

“…Moreover, I create a 1D array of Schr\"odinger cats states in facilitated by an optical lattice in the HCF and study its potential for applications in quantum sensing and quantum simulation in HCF. …”
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Co-electrolysis of seawater and carbon dioxide inside a microfluidic reactor to synthesize speciality organics by Rarotra, Saptak, Singh, Amit Kumar, Mandal, Tapas Kumar, Bandyopadhyay, Dipankar

“…The coupling of solar cell panels with electrodes generated a high-intensity electrical field across the electrodes at low voltage, which facilitated the co-electrolysis of CO2 and seawater. …”
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Host genotype and microbiome associations in co-occurring clonal and non-clonal kelp, Ecklonia radiata by Gonzalez, Sebastian Vadillo, Vranken, Sofie, Coleman, Melinda A., Wernberg, Thomas, Steinberg, Peter D., Marzinelli, Ezequiel Miguel

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Quantitative evaluation approach for translation of perceptual soundscape attributes: initial application to the Thai language by Watcharasupat, Karn N., Jaratjarungkiat, Sureenate, Lam, Bhan, Jitwiriyanont, Sujinat, Ooi, Kenneth, Ong, Zhen-Ting, Pichetpan, Nitipong, Akaratham, Kanyanut, Suthiwan, Titima, Rojtinnakorn, Monthita, Gan, Woon-Seng

“…Subsequent statistical analysis of the evaluation scores revealed acoustico-psycholinguistic properties of the translation candidates which were not previously identified by the expert panel and facilitated a more objective selection of the final translations for subsequent usage. …”
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Development of pigmented human skin constructs via 3D drop-on-demand bioprinting by Ng, Wei Long

“…This critical step facilitates the patterning of epidermal melanin units (EMUs, cell-cell interactions between the keratinocytes and melanocytes) at an optimal ratio and density. …”
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Matrix polysaccharides and SiaD diguanylate cyclase alter community structure and competitiveness of Pseudomonas aeruginosa during dual-species biofilm development with Staphylococ... by Chew, Su Chuen, Yam, Joey Kuok Hoong, Matysik, Artur, Seng, Zi Jing, Klebensberger, Janosch, Givskov, Michael, Doyle, Patrick, Rice, Scott A., Yang, Liang, Kjelleberg, Staffan

“…In contrast to the effect mediated by Psl, Pel reduced the effective crosslinking of the biofilm matrix and facilitated superdiffusivity in microcolony regions. …”
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Artificial photosynthesis by light absorption, charge separation, and multielectron catalysis by Đokić, Miloš, Soo, Han Sen

“…In this context, we showcase our team’s contributions in presenting a unique oxidative carbon-carbon bond cleavage reaction in aliphatic alcohols and biomass model compounds, under ambient atmospheric conditions, facilitated by vanadium photocatalysts. We offer this discovery as a promising alternative to water oxidation in an integrated AP system, which would concurrently generate both solar fuels and valuable solar chemicals.…”
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Waste palm leaves-based hydrochar support catalytic esterification of palm fatty acid distillate by Aliyu, Muhammad, Rashid, Umer, Wan Ab Karim Ghani, Wan Azlina, Mohd Salleh, Mohamad Amran, Hazmi, Balkis, Alharthi, Fahad A., Antunes, Elsa, Yu, Jianglong

“…The significant acidity density (18.57 mmol/g) facilitated the simultaneous esterification process. …”

Dual-functional witherite in improving chemical looping performance of iron ore and simultaneous adsorption of HCl in syngas at high temperature by Wang, Haiming, Liu, Guicai, Boon, Yinn Zhao, Veksha, Andrei, Giannis, Apostolos, Lim, Teik-Thye, Lisak, Grzegorz

“…The hexagonal structure of the BaFe12O19 facilitated the lattice oxygen mobility and thus improved greatly the redox performance of the IO. …”
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Barium aluminate improved iron ore for the chemical looping combustion of syngas by Wang, Haiming, Dou, Xiaomin, Veksha, Andrei, Liu, Wen, Giannis, Apostolos, Ge, Liya, Lim, Teik-Thye, Lisak, Grzegorz

“…Based on complementary characterization analyses, we found that more oxygen vacancies were formed in the modified OC due to the oxygen non-stoichiometry nature of the BaAl2O4 and its interaction with the iron oxide species. This interaction facilitated the rapid migration of the lattice oxygen in the bulk phase, thereby enhancing the reactivity and increasing the oxygen transport capacity of the OCs. …”
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The opioid receptor influences circadian rhythms in human keratinocytes through the β-arrestin pathway by Bigliardi, Paul, Junnarkar, Seetanshu, Markale, Chinmay, Lo, Sydney, Bigliardi, Elena, Kalyuzhny, Alex, Ong, Sheena, Dunn, Ray, Wahli, Walter, Bigliardi-Qi, Mei

“…Furthermore, we observed that β-arrestin-1 regulates the transcription of its target genes, including PER2, by facilitating histone-4 acetylation. Through the ChIP assay, we determined that Met-Enkephalin enhances β-arrestin-1 binding to acetylated H4 in the PER2 promoter. …”
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Hospitalised patients as stewards of their own antibiotic therapy: a qualitative analysis informing the strategic design of interventions to encourage shared decision-making in ter... by Guo, Huiling, Lye, David C., Ng, Tat Ming, Somani, Jyoti, Kwa, Andrea Lay Hoon, Chung, Jasmine Shimin, Chow, Angela

“…Understanding the facilitators and barriers to SDM can inform the design and implementation of interventions to empower these patients to engage in SDM on their antibiotic therapies. …”
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Quantum spin Hall effect and topological phase transition in InNxBiySb1−x−y/InSb quantum wells by Song, Zhigang, Bose, Sumanta, Fan, Weijun, Zhang, Dao Hua, Zhang, Yan Yang, Li, Shu Shen

“…These edge-states are facilitated by the time-reversal symmetry and they are robust against nonmagnetic impurity scattering. …”
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The history of Koi aquaculture in Singapore from 1965 to the present : translating translocated scientific knowledge from Japan into biosecurity by Tan, Delfinn Sweimay

“…The protocol was then transferred to Singapore and officials used a militarized concept of disease to translate the protocol into biosecurity regulations. This facilitated the formation of new koi-cultivation practices in the form of strict quarantine and certification measures that impacted farm practices and eventually the ornamental fish aquaculture industry in general. …”
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Agriculture and citadel in the Bronze Age Aegean: the view from western Anatolia by Maltas, T

“…Burial evidence from settlements with highly developed and corporate centralising structures, however, suggests that these ostensibly equalising institutions facilitated the emergence of high status and wealthy individuals. …”

Fabrication and biocompatibility evaluation of hydroxyapatite–polycaprolactone–gelatin composite nanofibers as a bone scaffold by Aminatun, None, Sujak M. K, Aisyah, Izak R, Djony, Hadi, Sofijan, Sari, Yessie Widia, Gunawarman, None, Cahyati, Nilam, Yusuf, Yusril, Che Abdullah, Che Azurahanim

“…The highest porosity percentage was (77.27 ± 11.57) %, and a sufficient degradation rate of up to 3.5 months facilitated the proliferation process of osteoblast cells. …”
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