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    Conception of conjugative cloning vectors. by Tan, Peng Fei.

    Published 2012
    “…Combining DNA sequencing technology with conjugation protocols, tri-parental mating was performed using 3 different Escherichia coli strains, S17-1 strain as the donor of a pUC vector with RP4 OriT incorporated, a PHL 628 strain possessing the ~7.8kb large fragment(MalA gene) and lastly a S17-1 Rifamycin-resistant strain being the collector of the recombinant shuttle plasmid. …”
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    Final Year Project (FYP)