Assessment of gut microbiome variations in the mother and twin infant of captive white-handed gibbons (Hylobates lar) reveals the presence of beneficial and nonpathogenic bacteria by Badrul Munir Md-Zain, Siti Hajar Azmi, Roberta Chaya Tawie Tingga, Millawati Gani, Mohamad Khairulmunir, Abd Rahman Mohd-Ridwan

“…Next-generation sequencing (NGS) of the 16S rRNA gene was performed. The NGS data were analyzed on the basis of amplicon sequence variation. …”
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Identification of lactic acid bacteria from chili bo, a Malaysian food ingredient by Leisner, Jørgen J., Pot, Bruno, Christensen, Henrik, Rusul, Gulam

“…Two other strains did not belong to any previously described 16S rRNA group of LAB and occupied an intermediate position between the L. casei/Pediococcus group and the Weissella group and species of Carnobacterium. …”
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Transcriptome analysis of ischemic stroke recovery induced by neural stem cell preconditioned with baicalein-enriched fraction of oroxylum indicum by Satar, Asmaa’ Mohd

“…The brain issues were harvested and snap-frozen using liquid nitrogen to homogenize the brain tissue for RNA extraction. The extracted RNA was analysed using microarray assay to reveal differentially expressed genes (DEGs), gene ontology (GO) and biological pathways related to neurological behavior improvement of the rats. …”
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A microfluidic platform to study pathogen-host interactions at single cell level by Zhang, Rui

“…The mRNA transcripts of rmd gene (an important gene involved in O antigen synthesis) of P. aeruginosa attached to single A549 cells were quantified using the 2D nucleic acid purification chip. …”
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Runx family genes in a cartilaginous Fish, the elephant Shark (Callorhinchus milii) by Nah, Giselle Sek Suan, Lim, Zhi Wei, Tay, Boon-Hui, Osato, Motomi, Venkatesh, Byrappa

“…Some of these CNEs and miRNA binding sites are absent in teleost fishes such as zebrafish and fugu. …”
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Synthesis and properties of amino- and guanidino-peptoid peptide nucleic acids by Lu, Xiao Wei

“…Peptide nucleic acid (PNA) is a synthetic mimic of DNA and RNA that can bind to complementary DNA and RNA with high affinity and sequence specificity. …”
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Unravelling gene regulation in the epidermis during differentiation by Anisa Banu Abdul Rahim

“…Polyamines are small polycationic molecules that are essential for many cellular processes including DNA replication, RNA modification and protein synthesis. The major polyamines in eukaryotic cells are putrescine (Put), spermidine (Spd) and spermine (Spm). …”
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Up-regulation of Mcl-1 and Bak by coronavirus infection of human, avian and animal cells modulates apoptosis and viral replication by Zhong, Yanxin., Liao, Ying., Fang, Shouguo., Tam, James P., Liu, Ding Xiang.

“…Interestingly, the onset of apoptosis occurred earlier in IBV-infected mammalian cells silenced with short interfering RNA targeting Mcl-1 (siMcl-1), and was delayed in cells silenced with siBak. …”
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A Coxsackievirus B1-mediated nonlytic extracellular vesicle-to-cell mechanism of virus transmission and its possible control through modulation of EV release by Jorfi, Samireh, Ansa-Addo, Ephraim A., Mariniello, Katia, Warde, Purva, Bin Senian, Ahmad Asyraf, Stratton, Dan, Bax, Bridget E., Levene, Michelle, Lange, Sigrun, Inal, Jameel

“…Inhibition of CVB1-mEV production, with calpeptin or siRNA knockdown of in HeLa cells limited spread of CVB1 suggesting these vesicles disseminate CVB1 virions to new host cells by a nonlytic EV-to-cell mechanism. …”
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MAIT cells play a role in liver tissue repair via growth factor secretion by Sayaf, K, Tasin, I, Akther, HD, Garner, LC, Ramamurthy, N, Hackstein, CP, Russo, FP, Klenerman, P

“…In an in vitro wound healing assay, supernatants of activated sorted MAIT cells accelerated wound closure, an effect that could be blocked by anti-VEGFR2. Interrogation of a scRNA-seq data from a human experimental model showed that MAIT cell expression of VEGF in vivo was detectable in regenerating liver tissue.…”

BRCA1 regulation of breast cancer cell motility by Hernandez Nuñez, ZE

“…These models were used in conjunction with the siRNA knockdown of candidate proteins from the <em>in-silico</em> analysis. siRNA knockdown of SART3 significantly enhanced motility in cells lacking BRCA’s ubiquitin ligase activity, highlighting its potential role in promoting cell motility and emphasising the regulatory role of BRCA1’s ubiquitin ligase activity in breast cancer progression.…”

Immunological screening of Lassa virus among health workers and contacts of patients of Lassa fever in Ondo State by Shaibu, JO, Salu, OB, Amoo, OS, Idigbe, I, Musa, AZ, Ezechi, OC, Abejegah, C, Ayodeji, O, Salako, BL, Omilabu, SA, Audu, RA

“…There was no detectable LASV RNA in the samples analyzed. <p><b>Conclusion</b></p> These findings suggest that majority of the health workers are naïve to the virus and hence may be prone to the viral infection. …”

The molecular pathogenesis of FUS mutations in amyotrophic lateral sclerosis using in vivo and in vitro models by Kent, L

“…Fused in sarcoma (FUS) is an RNA binding protein, and mutations in the FUS gene are responsible for approximately 4% of familial and &amp;LT;1% of sporadic ALS cases. …”

Structural and mechanistic insights into the Rel protein of Mycobacterium tuberculosis by Singal, Bharti

“…The structure of the MtRel TGS determined by NMR spectroscopy and titration with deacylated tRNA answered the long- argued question about interaction of the TGS domain to deacylated tRNA in the absence of ribosomal machinery using biophysical methods. …”
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R-loopDB: a database for R-loop forming sequences (RLFS) and R-loops by Jenjaroenpun, Piroon, Wongsurawat, Thidathip, Sutheeworapong, Sawannee, Kuznetsov, Vladimir A.

“…It provides a comprehensive annotation of Ensembl RLFS-positive genes including 93 454 protein coding genes, 12 480 long non-coding RNA and 7 568 small non-coding RNA genes and 7 554 pseudogenes. …”
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Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences by Sun, J, Philpott, M, Loi, D, Hoffman, G, Robson, J, Mehta, N, Calcutt, E, Gamble, V, Brown, T, Brown, T, Oppermann, U, Cribbs, AP

“…Single-cell transcriptomics, which utilises barcodes and unique molecular identifiers (UMIs) for polyA+ mRNA capture, is compromised by oligonucleotide synthesis errors. …”

Novel method of probe design for characterising unclassified microbial taxa in wastewater by Tan, Shi Ming

“…Taxonomic identification was made possible through phylogenetic assignment of full-length 16S rRNA gene sequences obtained from clone libraries. …”
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Super-enhancers, phase-separated condensates, and 3D genome organization in cancer by Tang, Seng Chuan, Vijayakumar, Udhaya, Zhang, Ying, Fullwood, Melissa Jane

“…Multiple transcription factors can bind to enhancer and promoter sequences and recruit a complex array of transcriptional co-activators and RNA polymerase II to effect transcriptional activation. …”
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Identification of potential common cyclic-di-GMP regulated biomarkers among three gram negative bacteria via transcriptomic and metabolomic profiling by Cai, Zhao

“…In this study, we applied RNA-sequencing (RNA-seq) and High Performance Liquid Chromatography (LC/MS) techniques to analyse the transcript-me and metabolome of Pseudomonas aeruginosa, Burkholderia cenocepacia and Klebsie-lla pneumoniae cells which are genetically modified to have either excessive or reduced intracellular c-di-GMP levels, in hope to discover possible common cross-species biomarkers. …”
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N6-Methyladenosine: a conformational marker that regulates the substrate specificity of human demethylases FTO and ALKBH5 by Zou, Shui, Toh, Joel D. W., Wong, Kendra H. Q., Gao, Yong-Gui, Hong, Wanjin, Woon, Esther C. Y.

“…N6-Methyladenosine (m6A) is currently one of the most intensively studied post-transcriptional modifications in RNA. Due to its critical role in epigenetics and physiological links to several human diseases, it is also of tremendous biological and medical interest. …”
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