Accelerating the design of biomimetic materials by integrating RNA-seq with proteomics and materials science by Guerette, Paul A, Hoon, Shawn, Seow, Yiqi, Raida, Manfred, Masic, Admir, Wong, Fong T, Ho, Vincent H B, Kong, Kiat Whye, Demirel, Melik C, Pena-Francesch, Abdon, Amini, Shahrouz, Tay, Gavin Z, Ding, Dawei, Miserez, Ali

“…Here we show that biomimetic engineering can be accelerated by integrating high-throughput RNA-seq with proteomics and advanced materials characterization. …”
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Different modes of regulation govern miRNA expression levels in Drosophila melanogaster by Lim, Mandy Yu Theng

“…Loquacious (Loqs) is a dsRNA-binding protein that binds Dicer-1 to cleave pre-miRNAs into short duplexes. …”
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Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs by Garcia-Guerra, A, Sathyaprakash, C, de Jong, O, Lim, W, Vader, P, El Andaloussi, S, Bath, J, Reine, J, Aoki, Y, Turberfield, A, Wood, MA, Rinaldi, C

“…Endogenous microRNAs (miRNAs) can be used to profile cell type and cell state, and are ideal inputs for RNA nanodevices. Here, we present CRISPR MiRAGE (miRNA-activated genome editing), a tool comprising a dynamic single-guide RNA that senses miRNA complexed with Argonaute proteins and controls downstream CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) activity based on the detected miRNA signature. …”

Intraperitoneal administration of mRNA encoding interleukin-12 for immunotherapy in peritoneal carcinomatosis by Arrizabalaga, L, Di Trani, CA, Fernández-Sendin, M, Bella, Á, Russo-Cabrera, JS, Gomar, C, Ardaiz, N, Belsue, V, González-Gomariz, J, Zalba, S, Gil-Korilis, A, Garrido, MJ, Melero, I, Aranda, F, Berraondo, P

“…To explore the therapeutic potential of this approach, mRNA complexes encoding single-chain interleukin-12 (IL12), an immunostimulatory molecule (mRNA-IL12), were investigated. mRNA-IL12-treated mice exhibited a significant survival advantage in models of peritoneal carcinomatosis and acquired immune memory, as shown upon subcutaneous rechallenge. …”

Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys by Mockenhaupt, Stefan, Grosse, Stefanie, Rupp, Daniel, Bartenschlager, Ralf, Grimm, Dirk

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Scaffold-mediated sustained, non-viral delivery of miR-219/miR-338 promotes CNS remyelination by Milbreta, Ulla, Lin, Junquan, Pinese, Coline, Ong, William, Chin, Jiah Shin, Shirahama, Hitomi, Mi, Ruifa, Williams, Anna, Bechler, Marie E., Wang, Jun, ffrench-Constant, Charles, Hoke, Ahmet, Chew, Sing Yian

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LSTrAP-Cloud : a user-friendly cloud computing pipeline to infer coexpression networks by Tan, Qiao Wen, Goh, William, Mutwil, Marek

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Recent advances in chitosan-based carriers for gene delivery by Cao, Ye, Tan, Yang Fei, Wong, Yee Shan, Liew, Melvin Wen Jie, Venkatraman, Subbu

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In Vivo RNA Delivery to Hematopoietic Stem and Progenitor Cells via Targeted Lipid Nanoparticles by Shi, Dennis, Toyonaga, Sho, Anderson, Daniel G

“…Here, we developed a c-kit (CD117) antibody-targeted lipid nanoparticle (LNP) that, following a single intravenous injection, can deliver RNA (both siRNA and mRNA) to HSCs in vivo in rodents. …”
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Inosine-containing mRNA induces an innate immune response and is translated with lower efficiency by Bao, Caroline

“…Inosine is a nucleoside formed by deamination of adenosine by adenosine deaminases acting on RNA (ADAR). ADAR editing activity is known to play a key role in modulating the host cell’s immune response to RNA. …”
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The enhancer RNA ARIEL activates the oncogenic transcriptional program in T-cell acute lymphoblastic leukemia by Tan, Shi Hao, Leong, Wei Zhong, Ngoc, Phuong Cao Thi, Tan, Tze King, Bertulfo, Fatima Carla, Lim, Mei Chee, An, Omer, Li, Zhenhua, Yeoh, Allen Eng Juh, Tenen, Daniel G., Sanda, Takaomi, Fullwood, Melissa Jane

“…Here, we elucidated the molecular functions of the noncoding RNA, ARID5B-inducing enhancer associated long noncoding RNA (ARIEL), in T-ALL pathogenesis. …”
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Multilayered siRNA nanoparticles as a delivery system for extended gene silencing effect by Ng, Alice Jie Ying

“…The 7 layered LbL NPs of HAp | PLR | siRNA | PLR | siRNA | PLR | siRNA | PLR configuration was 431.30 nm in hydrodynamic diameter with positive surface charge of 42.13 mV. …”
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Modulating macrophage phenotype by sustained microRNA delivery improves host‐implant integration by Lin, Junquan, Ibrahim Mohamed, Lin, Po Hen, Shirahama, Hitomi, Milbreta, Ulla, Sieow, Je Lin, Peng, Yanfen, Bugiani, Marianna, Wong, Siew Cheng, Levinson, Howard, Chew, Sing Yian

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Atypical DNA methylation, sRNA-size distribution, and female gametogenesis in Utricularia gibba by Cervantes-Pérez, Sergio Alan, Yong-Villalobos, Lenin, Florez-Zapata, Nathalia M. V., Oropeza-Aburto, Araceli, Rico-Reséndiz, Félix, Amasende-Morales, Itzel, Lan, Tianying, Martínez, Octavio, Vielle-Calzada, Jean Philippe, Albert, Victor A., Herrera-Estrella, Luis

“…The most studied DNA methylation pathway in plants is the RNA Directed DNA Methylation (RdDM), a conserved mechanism that involves the role of noncoding RNAs to control the expansion of the noncoding genome. …”
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Designing siRNA/chitosan-methacrylate complex nanolipogel for prolonged gene silencing effects by Cao, Ye, Tan, Yang Fei, Wong, Yee Shan, Aminuddin, Muhammad, Ramya, Bhuthalingam, Liew, Melvin Wen Jie, Liu, Jiaxin, Venkatraman, Subbu S.

“…Despite immense revolutionary therapeutics potential, sustaining release of active small interfering RNA (siRNA) remains an arduous challenge. The development of nanoparticles with siRNA sustained release capabilities provides an avenue to enhance the therapeutic efficacy of gene-based therapy. …”
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Deep transcriptome profiling reveals limited conservation of A-to-I RNA editing in Xenopus by Nguyen, Tram Anh, Heng, Joel Jia Wei, Ng, Yan Ting, Sun, Rui, Fisher, Shira, Oguz, Gokce, Kaewsapsak, Pornchai, Xue, Shifeng, Reversade, Bruno, Ramasamy, Adaikalavan, Eisenberg, Eli, Tan, Meng How

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Use of 16S rRNA sequencing to determine phylum Chloroflexi diversity in substratum habitats. by Tang, Peggy Pei Yi.

“…16S rRNA sequencing was used to determine Chloroflexi diversity on 12 soil samples taken from an underground tunnel in Sweden. …”
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Ribozyme Mediated gRNA Generation for In Vitro and In Vivo CRISPR/Cas9 Mutagenesis by Lee, Raymond Teck Ho, Ng, Ashley Shu Mei, Ingham, Philip William

“…We have tested this by comparing the efficiency of mutagenesis of gRNA constructs with and without ribozymes and also generated a transgenic zebrafish expressing gRNA using a heat shock promoter (RNA polymerase II-dependent promoter) that was able to induce mutagenesis of its target. …”
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Negative auto-regulation of myostatin expression is mediated by SMAD3 and microRNA-27 by Ge, XiaoJia, McFarlane, Craig, Vajjala, Anuradha, Arigela, Harikumar, Lokireddy, Sudarsanareddy, Bonala, Sabeera, Manickam, Ravikumar, Kambadur, Ravi, Sharma, Mridula

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Knocking down MEG3 long non-coding RNA to Counteract Necroptosis in vitro by Kemp, E