Multiple functions of shutdown in Drosophila germline development by Chong, Jeronica Wen Hui

“…In animals, maintenance of the germline genomic integrity is crucial for accurate transmission of genetic information to the next generation. A unique small RNA pathway known as the PIWI-interacting RNA (piRNA) pathway is present in the animal germline to prevent transposable elements from subverting genomic integrity. …”
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Characterization of the translational regulation of BIM 3’ UTR by Ho, Jessie Jia Xin

“…Genomic variations of the BIM gene were shown to account for drug resistance. MicroRNAs (miRNA) play a role in post-transcriptional regulation by binding to the messenger RNA (mRNA) 3’ untranslated region (UTR) of protein coding genes which include BIM. …”
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Development of immune-modulatory RNAS to regulate RIG-I-like receptors mediated antiviral immune response for therapy and vaccination by Yong, Hui Yee

“…In this thesis, the development of potent innate immune modulatory RNA (ImmRNA), capable of triggering a robust type I interferon signalling in human cells is reported. …”
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PRMT5 controls the innate immune response by Zhang, C

“…</p> <p>Transfection of HCT116 cells with intron-retained genes resulted in elevated dsRNA and IFN-β levels. Validation of the dsRNA structure of RI transcripts was also attempted using dsRNA immunoprecipitation (RIP). …”

Regulatory RNAs discovered in unexpected places by Pek, Jun Wei, Okamura, Katsutomo

“…Recent studies have discovered both small and long noncoding RNAs (ncRNAs) encoded in unexpected places. These ncRNA genes were surprises at the time of their discovery, but many quickly became well-accepted families of functional regulatory RNA species. …”
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Molecular architecture of the Chikungunya virus replication complex by Tan, Yaw Bia, Chmielewski, David, Law, Michelle Cheok Yien, Zhang, Kuo, He, Yu, Chen, Muyuan, Jin, Jing, Luo, Dahai

“…To better understand how positive-strand (+) RNA viruses assemble membrane-associated replication complexes (RCs) to synthesize, process, and transport viral RNA in virus-infected cells, we determined both the high-resolution structure of the core RNA replicase of chikungunya virus and the native RC architecture in its cellular context at subnanometer resolution, using in vitro reconstitution and in situ electron cryotomography, respectively. …”
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Structural characterization of the full-length Hantaan virus polymerase by Keown, JR, Carrique, L, Nilsson-Payant, BE, Fodor, E, Grimes, JM

“…Hantaviridae contain a viral RNA-dependent RNA polymerase that replicates and transcribes the viral genome. …”

Investigation of NOT1 proteins in regulating gene expression in plasmodium falciparum by Liu, Ying

“…The knockout of Not1.1 affects recruitment of the elongating form of RNA Polymerase II, which however is not significantly correlated to differential mRNA abundance, suggesting the role of NOT1.1 in post-transcriptional regulation. …”
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Detect inosine using nanopore sequencing by Nguyen, Tram Anh

“…Adenosine to Inosine (A-to-I) editing is one of the most prevalent post-transcriptional RNA modifications. It plays numerous crucial roles in determining a cell's fate. …”
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Structural and functional characterisation of chikungunya virus replication complex dynamics by Tan, Yaw Bia

“…Chikungunya virus (CHIKV) is a 12 kilobase (kb) positive sense single strand RNA (+ssRNA) mosquito-borne virus. Through mosquito bites, the infected victims develop Chikungunya Fever (CHIKF) associated with symptoms like acute fever and painful polyarthritis. …”
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Development of CRISPR gene therapy for retinal degenerations by Fry, LE

“…The orientation of the guide RNA (gRNA) relative to Staphylococcus aureus-derived SaCas9 within an all-in-one AAV-CRISPR vector was compared. …”

Exon skipping is correlated with exon circularization by Kelly, S, Greenman, C, Cook, PR, Papantonis, A

“…Here, we stimulated primary human endothelial cells with tumor necrosis factor α or tumor growth factor β, purified RNA, generated &gt; 2.4 billion RNA-seq reads, and used a custom pipeline to characterize circular RNAs derived from coding exons. …”

Robust delivery of RIG-I agonists using extracellular vesicles for anti-cancer immunotherapy by Peng, Boya, Nguyen, Trinh Mai, Jayasinghe, Migara Kavishka, Gao, Chang, Pham, Thach Tuan, Vu, Luyen Tien, Yeo, Eric Yew Meng, Yap, Gracemary, Wang, Lingzhi, Goh, Boon Cher, Tam, Wai Leong, Luo, Dahai, Le, Minh TN

“…The RIG-I pathway can be activated by RNA containing 5' triphosphate, leading to type I interferon release and immune activation. …”
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Scaffold-mediated non-viral delivery platform for CRISPR/Cas9-based genome editing by Chin, Jiah Shin, Chooi, Wai Hon, Wang, Hongxia, Ong, William, Leong, Kam W., Chew, Sing Yian

“…Here, we developed a scaffold that delivers the CRISPR/Cas9 components (i.e. single guide RNA (sgRNA) and Cas9 protein complexes) in a localized and non-viral manner. …”
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Sequence-Dependent & -Independent Effects of Intron-Mediated Enhancement (IME) by Kowal, Emma J. K.

“…Introns are ubiquitous features of eukaryotic genes, and their precise removal from pre-mRNA transcripts by the spliceosome is an essential step in gene expression. …”
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Red blood cell extracellular vesicles incorporated into 3D printed scaffolds for spinal cord injury regeneration by Huang, Chongquan, Jayasinghe, Migara Kavishka, Lau, Kieran, Thi Nguyet Minh Le, Chew, Sing Yian

“…In this study, we extracted red blood cell extracellular vesicles (RBCEVs) and loaded siRNA/miRNA into them as the primary gene carrier to achieve 1) and 2). …”
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Characterization and identification of Oya virus, a Simbu serogroup virus of the genus Bunyavirus, isolated from a pig suspected of Nipah virus infection by Kono, Y., Yusnita, Y., Mohd Ali, A. R., Maizan, M., Sharifah, S. H., Fauzia, O., Kubo, M., Aziz, A. J.

“…A common primer set for S-RNA of the Simbu serogroup of the genus Bunyavirus was able to amplify a cDNA from Oya virus RNA. …”

Single cell genomics data analyses reveal novel biological insights by Hu, X

“…<p>Single cell RNA-Seq is one type of single cell –omics experiment. …”

Mapping epigenetic modifications by sequencing technologies by Chen, X, Xu, H, Shu, X, Song, C-X

“…Thus far, chemical modifications on histones, DNA, and RNA have emerged as three important building blocks of epigenetic modifications. …”

The first complete mitochondrial genome of the critically endangered Malaysian giant turtle, Orlitia borneensis (Testudines: Geoemydidae) by Salleh, Mohd Hairul Mohd, Esa, Yuzine

“…The assembled mitochondrial genome includes 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA genes (rRNAs), and one control region. …”