Functional analysis of root-preferential oil palm metallothionein promoter in tobacco by Masura, Subhi Siti, Shaharuddin, Noor Azmi, Masani, Mat Yunus Abdul, Chan, Kuang-Lim, Low, Eng-Ti Leslie, Chan, Pek-Lan, Rahmah, Abdul Rahman Siti, Amiruddin, Nadzirah, Abdullah, Mohd Puad, Azzeme, Azzreena Mohamad, Parveez, Ghulam Kadir Ahmad, Abd Rasid, Omar

“…We have characterised the promoter from an oil palm metallothionein gene (EgMT) and performed a serial 5' deletion analysis to identify the region(s) essential for transgenes expression in roots. …”

Changes in gene expression in human skeletal stem cells transduced with constitutively active Gsα correlates with hallmark histopathological changes seen in fibrous dysplastic bone... by Raimondo, Domenico, Remoli, Cristina, Astrologo, Letizia, Burla, Romina, La Torre, Mattia, Vernì, Fiammetta, Tagliafico, Enrico, Corsi, Alessandro, Del Giudice, Simona, Persichetti, Agnese, Giannicola, Giuseppe, Robey, Pamela G., Riminucci, Mara, Saggio, Isabella

Get full text

Gene expression regulation by well-known non-coding RNAs by Tan, Jiazi

“…From there, we could screen for genes important for XCI by rescuing the differentiated transgenic ES cells by gene silencing. …”
Get full text

Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root plasticity response to nitrogen by Huang, Y, Ji, Z, Tao, Y, Wei, S, Jiao, W, Fang, Y, Jian, P, Shen, C, Qin, Y, Zhang, S, Li, S, Liu, X, Kang, S, Tian, Y, Song, Q, Harberd, NP, Wang, S, Li, S

“…In this study, we identified RNR10 (REGULATOR OF N-RESPONSIVE RSA ON CHROMOSOME 10) as the causal gene that underlies the significantly different root developmental plasticity in response to changes in N level exhibited by the indica (Xian) and japonica (Geng) subspecies of rice. …”

Induction of apoptosis and the signalling pathways involved by dillenia suffruticosa dichloromethane root extract in mcf-7 and mda-mb-231 breast cancer cells by Foo, Jhi Biau

“…Previous study reported that dichloromethane extract of D. suffruticosa root (DCM-DS) was the most cytotoxic towards breast cancer cells. …”
Get full text

Regulation of Burkholderia cenocepacia biofilm formation by RpoN and the c-di-GMP effector BerB by Fazli, Mustafa, Rybtke, Morten, Steiner, Elisabeth, Weidel, Elisabeth, Berthelsen, Jens, Groizeleau, Julie, Bin, Wu, Zhi, Boo Zhao, Yaming, Zhang, Kaever, Volkhard, Givskov, Michael, Hartmann, Rolf W., Eberl, Leo, Tolker-Nielsen, Tim

Get full text
Get full text

Assessment of novel pre-erythrocytic malaria vaccines in humans by Venkatraman, N

“…This provides the first evidence of proof-of-concept that this vaccine regime should be tested in malaria-endemic populations.…”

Antidiabetic activity of Curculigo latifolia extracts in vitro and in vivo studies by Ishak, Nur Akmal

“…The regulatory effects of C. latifolia fruit:root extracts on genes involved in glucose and lipid metabolisms were further studied. …”
Get full text

Engineering control of rhizobacteria for plant growth promotion by Green, PWL

“…Use of this signal to induce bacterial gene expression was tested in the model cereal-associative rhizobium A. …”

Manufacturing mesenchymal stromal cells in a microcarrier-microbioreactor platform can enhance cell yield and quality attributes: case study for acute respiratory distress syndrome by Krupczak, Brandon, Farruggio, Camille, Van Vliet, Krystyn J.

“…Next, we identified 24 genes implicated in a therapeutic role for a specific motivating indication, acute respiratory distress syndrome (ARDS); expression of these genes served as our pCQAs for initial in vitro evaluation of product potency. …”
Get full text

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system by Shen, Yunbing, Jiang, Long, Iyer, Vaishnavi Srinivasan, Raposo, Bruno, Dubnovitsky, Anatoly, Boddul, Sanjaykumar V., Kasza, Zsolt, Wermeling, Fredrik

“…CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a CRISPR-targeted cell population will not show a uniform genotype of the targeted gene. …”
Get full text

Ectomycorrhizal community composition, diversity, and their environmental drivers across common UK temperate woodlands by Ong, Wei Sern

“…ECM richness, diversity and taxonomic community composition were determined by Sanger sequencing of the fungal ITS gene from collected ECM root tip samples, while functional traits and morphology of the ectomycorrhizas were also recorded. …”
Get full text

Archaeal proline dehydrogenase from Pyrobaculum aerophilum : sequence comparison. by Kik, Shian Chi.

“…U-96 and L-proline dehydrogenase from Pyrococcus horikoshii with root mean square deviation of 2.043 and 1.983 respectively. …”
Get full text

Validating predictive sequence analysis in polyketide stereochemical determination though the synthesis of stambomycin D by Lim, J

“…<p>In recent years, predictive gene sequence analysis of biosynthetic enzymes has emerged as a powerful tool for the stereochemical determination of natural products. …”

Detection and characterisation of a sixth Candida auris clade in Singapore: a genomic and phenotypic study by Suphavilai, Chayaporn, Ko, Karrie Kwan Ki, Lim, Kar Mun, Tan, Mei Gie, Boonsimma, Patipan, Chu, Joash Jun Keat, Goh, Sui Sin, Rajandran, Prevena, Lee, Lai Chee, Tan, Kwee Yuen, Bushra Binte Shaik Ismail, Aung, May Kyawt, Yang, Yong, Sim, Jean Xiang Ying, Venkatachalam, Indumathi, Cherng, Benjamin Pei Zhi, Spruijtenburg, Bram, Chan, Kian Sing, Oon, Lynette Lin Ean, Tan, Ai Ling, Tan, Yen Ee, Wijaya, Limin, Tan, Ban Hock, Ling, Moi Lin, Koh, Tse Hsien, Meis, Jacques F., Tsui, Clement Kin Ming, Nagarajan, Niranjan

“…Antifungal susceptibility testing was done and antifungal resistance genes, mating-type locus, and chromosomal rearrangements were characterised from the WGS data of the three investigated isolates. …”
Get full text

Morphological multiparameter filtration and persistent homology in mitochondrial image analysis by Chung, Yu-Min, Hu, Chuan-Shen, Sun, Emily, Tseng, Henry C.

“…Using this information, we also develop a connectivity index that describes the morphology of the branching patterns. As proof of concept, we utilize this approach to study how mitochondrial networks are altered by genetic changes in the Optineurin gene. …”
Get full text

Detection of single-nucleotide polymorphisms in Plasmodium falciparum by PCR primer extension and lateral flow immunoassay by Moers, APHA, Hallett, R, Burrow, R, Schallig, HDFH, Sutherland, CJ, van Amerongen, A

“…Primer extension methods (PEXT) were developed to identify 4 SNPs in P. falciparum positioned at amino acids 86, 184, and 1246 of the P. falciparum multidrug resistance 1 gene (pfmdr1) and amino acid 76 of the chloroquine resistance transporter gene (pfcrt). …”

Evolutionary origin of the chordate nervous system revealed by amphioxus developmental trajectories by Dai, Y, Zhong, Y, Liu, H, Pan, R, Yuan, L, Du, J, Li, M, Wang, X, Shi, C, Liu, G, Zhu, P, Shimeld, S, Zhou, X, Li, G

“…We identified many new amphioxus cell types, including homologues to the vertebrate hypothalamus and neurohypophysis, rooting the evolutionary origin of these structures. …”

Alleviation of off-target effects from vector-encoded shRNAs via codelivered RNA decoys by Mockenhaupt, Stefan, Grosse, Stefanie, Rupp, Daniel, Bartenschlager, Ralf, Grimm, Dirk

“…Our combination of shRNA and TuD in a single bicistronic gene transfer vector derived from Adeno-associated virus (AAV) enables a wide range of applications, including gene therapies. …”
Get full text
Get full text

Scaffold-mediated sustained, non-viral delivery of miR-219/miR-338 promotes CNS remyelination by Milbreta, Ulla, Lin, Junquan, Pinese, Coline, Ong, William, Chin, Jiah Shin, Shirahama, Hitomi, Mi, Ruifa, Williams, Anna, Bechler, Marie E., Wang, Jun, ffrench-Constant, Charles, Hoke, Ahmet, Chew, Sing Yian

Get full text