Characterization of the near native conformational states of the SAM domain of Ste11 protein by NMR spectroscopy by Gupta, Sebanti, Bhattacharjya, Surajit

“…SAM domain of the Ste11 protein of budding yeast plays important roles in mitogen-activated protein kinase cascades. …”
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On the total synthesis of complex alkaloids by Shennan, BDA

“…Following a concerted investigation into C−H oxidation for the former and a transfer of chirality approach for the latter, a Baker’s yeast reduction is adopted as an efficient route towards an enantioenriched, oxidised pyrrolidine building block which is advanced to a pentacyclic intermediate.…”

The bHLH transcription factor OsPRI1 activates the Setaria viridis PEPC1 promoter in rice by Carvalho, P, Gomes, C, Gonçalves, I, Lourenço, TF, Vlad, D, Langdale, JA, Saibo, NJM

“…To engineer C₄ into a C₃ plant, it is essential to understand how C₄ genes, such as <i>phosphoenolpyruvate carboxylase (PEPC1)</i>, are regulated to be expressed at high levels and in a cell-specific manner.</li> <li>Yeast one-hybrid screening was used to show that OsPRI1, a rice bHLH transcription factor involved in iron homeostasis, binds to the <i>Setaria viridis PEPC1</i> promoter. …”

Recent insight into the advances and prospects of microbial lipases and their potential applications in industry by Eskandari, Azadeh, Thean, Chor Leow, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…This updated review presents new knowledge about various origins of microbial lipases, such as fungi, bacteria, and yeast. It highlights both the traditional and modern purification methods, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, the aqueous two-phase system (ATPS), and aqueous two-phase flotation (ATPF), moreover, delves into the diverse applications of microbial lipases across several industries, such as food, vitamin esters, textile, detergent, biodiesel, and bioremediation. …”

A study of GPS based attitude determination technique by Dai, Lin

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CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome by Dalvie, Neil C., Lorgeree, Timothy R., Yang, Yuchen, Rodriguez-Aponte, Sergio A., Whittaker, Charles A., Hinckley, Joshua A., Clark, John J., Del Rosario, Amanda M., Love, Kerry R., Love, J. C.

“…Background The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. …”
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Antibody-directed evolution reveals a mechanism for enhanced neutralization at the HIV-1 fusion peptide site by Banach, Bailey B, Pletnev, Sergei, Olia, Adam S, Xu, Kai, Zhang, Baoshan, Rawi, Reda, Bylund, Tatsiana, Doria-Rose, Nicole A, Nguyen, Thuy Duong, Fahad, Ahmed S, Lee, Myungjin, Lin, Bob C, Liu, Tracy, Louder, Mark K, Madan, Bharat, McKee, Krisha, O’Dell, Sijy, Sastry, Mallika, Schön, Arne, Bui, Natalie, Shen, Chen-Hsiang, Wolfe, Jacy R, Chuang, Gwo-Yu, Mascola, John R, Kwong, Peter D, DeKosky, Brandon J

“…Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. …”
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Crystal structure of the FK506 binding domain of plasmodium falciparum FKBP35 in complex with FK506 by Kotaka, Masayo, Ye, Hong, Alag, Reema, Hu, Guangan, Bozdech, Zbynek, Preiser, Peter Rainer, Yoon, Ho Sup, Lescar, Julien

“…It binds to a α/β protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. …”
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Finding transcription factor binding motifs for coregulated genes by combining sequence overrepresentation with cross-species conservation by Jia, Hui., Li, Jinming.

“…Compared with the prevailing motif finding software, our method has some advantages in finding transcriptional factor binding motifs for potential coregulated genes if the gene upstream sequences of multiple closely related species are available. Although we used yeast genomes to assess our method in this study, it might also be applied to other organisms if suitable related species are available and the upstream sequences of coregulated genes can be obtained for the multiple closely related species.…”
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Niemann-Pick type c disease: pathogenesis and therapy by Colaco, A

“…To gain further insight into the function of NPC1 and proteins it interacts with we used the yeast orthologue, Ncr1p. I recreated the Δncr1 mutant and characterized the mutant using an array of systematic screens to identify different processes and pathways that may play a role in NPC pathogenesis. …”

Isolation of Bifidobacteria infantis and its antagonistic activity against ETEC 0157 and Salmonella typhimurium S-285 in weaning foods by Mohd Yusof, Rokiah, Haque, Formuzul, Ismail, Maznah, Hassan, Zaiton

“…Trypticase phytone peptone yeast extract agar (TPY) was used as a selective media for the isolation. …”

Structural investigation, computational analysis, and theoretical cryoprotectant approach of antifreeze protein type IV mutants by Eskandari, Azadeh, Leow, Thean Chor, Abdul Rahman, Mohd Basyaruddin, Oslan, Siti Nurbaya

“…In this in silico study, the helical peptide afp1m from an Antarctic yeast AFP was modeled into a sculpin AFPIV, to replace each of its four α-helices in turn, using various computational tools. …”

Design of system for parking and repositioning of bikes in a public bike-sharing system by Siong, Jun Wei

“…In the case study of this report, the proposed model is shown to be able to determine a bike reallocation plan that incurs the least cost. Also, the model provides information of the quantity of bikes to reallocate and when to reallocate to incur the least cost. …”
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Globalization and environmental quality : insights from 128 countries by Le, Ha Chi

“…Fixed Effects (FE) is employed as the main estimation technique while panel Ordinary Least Squares (OLS), Feasible Generalized Least Squares (FGLS) and Driscoll-Kraay (D-K) standard errors estimations are employed as robustness check. …”
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A comparative study on parameter recovery of three approaches to structural equation modeling by Malhotra, Naresh K., Tomiuk, Marc A., Hwang, Heungsun, Kim, Youngchan, Hong, Sungjin

“…Traditionally, two approaches have been employed for structural equation modeling: covariance structure analysis and partial least squares. A third alternative, generalized structured component analysis, was introduced recently in the psychometric literature. …”
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Geometry of single axis motions using conic fitting by Jiang, G, Tsui, H-T, Quan, L, Zisserman, A

“…The main advantage is that determining only five parameters of a conic from one corresponding point over at least five views is simpler and more robust than determining a fundamental matrix from two views or a trifocal tensor from three views. …”

Microbial lipid production from food waste saccharified liquid and the effects of compositions by Ma, Xiaoyu, Gao, Zhen, Gao, Ming, Ma, Yingqun, Ma, Hongzhi, Zhang, Min, Liu, Yu, Wang, Qunhui

“…The residues of food waste after removing waste cooking oils could be converted to microbial lipid by oleaginous yeasts and microbial lipid could be used as a raw material of biodiesel with waste cooking oils. …”
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R-loopDB: a database for R-loop forming sequences (RLFS) and R-loops by Jenjaroenpun, Piroon, Wongsurawat, Thidathip, Sutheeworapong, Sawannee, Kuznetsov, Vladimir A.

“…It includes genome-scale prediction of RLFSs for humans, six other animals and yeast. Using the extended quantitative model of RLFSs (QmRLFS), we significantly increased the number of RLFSs predicted in the human genes and identified RLFSs in other organism genomes. …”
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Expression of recombinant geobacillus SP a-amylase in pichia pastoris GS 115 by Gandhi, Sivasangkary

“…Pichia pastoris is a currently well established methanol utilizing yeast expression system. The aim of the studies was to produce a recombinant Geobacillus sp. α-amylase SR74 in P. pastoris GS115 and characterize the biochemical properties of the purified recombinant α-amylase. …”
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Measuring shear wave velocity using bender elements by Leong, Eng Choon, Yeo, Sir Hoon, Rahardjo, Harianto

“…The interpretation of the bender element test can be improved if two performance criteria are adopted: (1) a signal-to-noise ratio of at least 4 dB for the receiver signal, and (2) a wave path length to wavelength ratio of at least 3.33. …”
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