Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm Genetic Requirements in Enterococcus faecalis by Afonina, Irina, Ong, June, Chua, Jerome, Lu, Timothy, Kline, Kimberly A

“…This system can silence genes involved in biofilm formation and antibiotic resistance and can be used to interrogate gene essentiality. …”
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Comparative Genomics of Enterococci: Variation in Enterococcus faecalis, Clade Structure in E. faecium, and Defining Characteristics of E. gallinarum and E. casseliflavus by Palmer, Kelli L., Godfrey, Paul, Griggs, Allison, Kos, Veronica N., Zucker, Jeremy, Desjardins, Christopher A., Cerqueira, Gustavo, Gevers, Dirk, Walker, Suzanne, Wortman, Jennifer, Feldgarden, Michael, Haas, Brian J., Birren, Bruce W., Gilmore, Michael S.

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A conserved helicase processivity factor is needed for conjugation and replication of an integrative and conjugative element by Thomas, Jacob, Lee, Catherine Ann, Grossman, Alan Davis

“…Integrative and conjugative elements (ICEs) are agents of horizontal gene transfer and have major roles in evolution and acquisition of new traits, including antibiotic resistances. ICEs are found integrated in a host chromosome and can excise and transfer to recipient bacteria via conjugation. …”
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Self-assembled thin films with tunable release kinetics for biomedical applications by Hsu, Bryan Boen

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Biophysical characterization of natural antimicrobials towards development of antibacterial skin therapies by Valle González, Elba Ruth

“…While direct inhibition of C. acnes and S. aureus can improve therapeutic outcomes, this goal is difficult to achieve due to the growing rise of antibiotic-resistant strains and current therapies rely mainly on ameliorating disease symptoms. …”
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SINTESIS �-TERPINEOL MELALUI HIDRASI �-PINENA DENGAN KATALIS HOMOGEN (SA, MCA, TCA) DAN HETEROGEN (ZHY, TCA/ZHY) SERTA UJI AKTIVITAS �-TERPINEOL SEBAGAI ZAT ANTIBAKTERI... by , Dra. Nanik Wijayati, M.Si., , Prof. Dr. rer. nat. Harno Dwi Pranowo, M.S.

“…The existence of product of isomerization reaction causes antibacterial activity of the α-terpineol obtained from the hydration reaction are smaller than those of the standar terpineol or commercial antibiotic compound (e.g. amikacin).…”

Neuroendocrine tumour of the lung: a diagnostic challenge / Nur Hidayah Bahrom … [et al.] by Bahrom, Nur Hidayah, Ramli, Anis Safura, Samsudin, Nor Suraya, Mohamad Ali, Norliana Dalila, Bakar, Nor Salmah

“…Diagnosis of bronchiectasis was made and he was treated with antibiotic and tranexamic acid. Due to financial difficulties, his care was transferred to a university respiratory clinic in June 2018. …”
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Adjunctive dexamethasone in bacterial meningitis: a meta-analysis of individual patient data. by van de Beek, D, Farrar, J, de Gans, J, Mai, N, Molyneux, E, Peltola, H, Peto, T, Roine, I, Scarborough, M, Schultsz, C, Thwaites, G, Tuan, P, Zwinderman, A

“…Dexamethasone had no effect in any of the prespecified subgroups, including specific causative organisms, pre-dexamethasone antibiotic treatment, HIV status, or age. Pooling of the mortality data with those of all other published trials did not significantly change the results. …”

‘Caveat emptor’: the cautionary tale of endocarditis and the potential pitfalls of clinical coding data—an electronic health records study by Fawcett, N, Young, B, Peto, L, Quan, TP, Gillott, R, Wu, J, Middlemass, C, Weston, S, Crook, DW, Peto, TEA, Muller-Pebody, B, Johnson, A, Walker, AS, Sandoe, J

“…Electronic health records have been used to explore the impact of changing guidance on antibiotic prophylaxis for dental procedures on incidence, but limited data on the accuracy of the diagnostic codes exists. …”

Dual predation by bacteriophage and Bdellovibrio bacteriovorus can eradicate escherichia coli prey in situations where single predation cannot by Hobley, L, Summers, JK, Till, R, Milner, DS, Atterbury, RJ, Stroud, A, Capeness, MJ, Gray, S, Leidenroth, A, Lambert, C, Connerton, I, Twycross, J, Baker, M, Tyson, J, Kreft, JU, Sockett, RE

“…This suggests that combinatorial predator therapy could overcome problems of phage resistance.IMPORTANCE With increasing levels of antibiotic resistance, the development of alternative antibacterial therapies is urgently needed. …”

Intravenous doxycycline, azithromycin, or both for severe scrub typhus by Varghese, GM, Dayanand, D, Gunasekaran, K, Kundu, D, Wyawahare, M, Sharma, N, Chaudhry, D, Mahajan, SK, Saravu, K, Aruldhas, BW, Mathew, BS, Nair, RG, Newbigging, N, Mathew, A, Abhilash, KPP, Biswal, M, Prasad, AH, Zachariah, A, Iyadurai, R, Hansdak, SG, Sathyendra, S, Sudarsanam, TD, Prakash, JAJ, Manesh, A, Mohan, A, Tarning, J, Blacksell, SD, Peerawaranun, P, Waithira, N, Mukaka, M, Cheah, PY, Peter, JV, Abraham, OC, Day, NPJ

“…<p><strong>BACKGROUND:</strong> The appropriate antibiotic treatment for severe scrub typhus, a neglected but widespread reemerging zoonotic infection, is unclear.…”

Studies on the Bacterial Potassium efflux system Kef in Shewanella denitrificans SdKef by Grayer, S

“…<p>The glutathione-gated potassium efflux system (Kef) is a K⁺/H⁺ antiporter found in the majority of Gram negative pathogens, which shows potential as a novel antibiotic target. Kef plays a vital role in the protection of bacteria against toxic electrophiles through the regulation of cytoplasmic pH. …”

Cost-effectiveness of screening, decolonisation and isolation strategies for carbapenem-resistant Enterobacterales and methicillin-resistant Staphylococcus aureus infections in hos... by Allel, K, Garcia, P, Peters, A, Munita, J, Undurraga, EA, Yakob, L

“…Evidence for sex differences among antibiotic resistant bacterial infections is increasing but a focus on policy implications is needed. …”

Characterization and genomic analysis of the lytic bacteriophage vB_EclM_HK6 as a potential approach to biocontrol the spread of Enterobacter cloacae contaminating food by Temsaah, HR, Azmy, AF, Ahmed, AE, Elshebrawy, HA, Kasem, NG, El-Gohary, FA, Lood, C, Lavigne, R, Abdelkader, K

“…Background: Increased prevalence of Enterobacter cloacae within food products underscores food as an underexplored reservoir for antibiotic resistance, thus requiring particular intervention. …”

Whole-genome sequencing to determine transmission of Neisseria gonorrhoeae: an observational study by De Silva, D, Peters, J, Cole, K, Cole, M, Cresswell, F, Dean, G, Dave, J, Thomas, D, Foster, K, Waldram, A, Wilson, D, Didelot, X, Grad, Y, Crook, D, Peto, T, Walker, A, Paul, J, Eyre, D

“…New approaches are urgently required to address increasing rates of gonorrhoea and the emergence and global spread of antibiotic-resistant Neisseria gonorrhoeae. We used whole-genome sequencing to study transmission and track resistance in N gonorrhoeae isolates.We did whole-genome sequencing of isolates obtained from samples collected from patients attending sexual health services in Brighton, UK, between Jan 1, 2011, and March 9, 2015. …”

Distinguishing bacterial versus non-bacterial causes of febrile illness - A systematic review of host biomarkers by Leticia Fernandez-Carballo, B, Escadafal, C, MacLean, E, Kapasi, AJ, Dittrich, S

“…A simple tool for distinguishing bacterial versus non-bacterial infections would radically improve patient management and reduce indiscriminate antibiotic use. Diagnostic tests based on host biomarkers can play an important role here, and a target product profile (TPP) was developed to guide development. …”

Population-level faecal metagenomic profiling as a tool to predict antimicrobial resistance in Enterobacterales isolates causing invasive infections: An exploratory study across Ca... by Auguet, OT, Niehus, R, Gweon, HS, Berkley, JA, Waichungo, J, Njim, T, Edgeworth, JD, Batra, R, Chau, K, Swann, J, Walker, SA, Peto, TEA, Crook, DW, Lamble, S, Turner, P, Cooper, BS, Stoesser, N

“…Capacity for individual-level surveillance remains limited in many countries, whilst population-level surveillance approaches could inform empiric antibiotic treatment guidelines. Methods In this exploratory study, a novel approach to population-level prediction of AMR in Enterobacterales clinical isolates using metagenomic (Illumina) profiling of pooled DNA extracts from human faecal samples was developed and tested. …”

Barriers and enablers to blood culture sampling in Indonesia, Thailand and Viet Nam: a Theoretical Domains Framework-based survey by Suntornsut, P, Asadinia, KS, Limato, R, Tamara, A, Rotty, LWA, Bramanti, R, Nusantara, DU, Nelwan, EJ, Khusuwan, S, Suphamongkholchaikul, W, Chamnan, P, Piyaphanee, W, Vu, HTL, Nguyen, YH, Nguyen, KH, Pham, TN, Le, QM, Vu, VH, Chau, DM, Vo, DETH, Harriss, EK, van Doorn, HR, Hamers, RL, Lorencatto, F, Atkins, L, Limmathurotsakul, D

“…<p><strong>Objective</strong> Blood culture (BC) sampling is recommended for all suspected sepsis patients prior to antibiotic administration. We examine barriers and enablers to BC sampling in three Southeast Asian countries.…”

Individual and collective behaviour of bacteria under oxidative stress by Choudhary, D

“…Bacteria frequently experience oxidative stress during aerobic growth, host immune attack, microbiome interactions, and under antibiotic treatment. Here, we study bacteria under hydrogen peroxide (H2O2) and observe that individual cells exhibit variability in their oxidative stress response. …”

Fecal microbiota profiles in treatment-naïve pediatric inflammatory bowel disease – associations with disease phenotype, treatment, and outcome by Olbjørn, C, Småstuen, MC, Thiis-Evensen, E, Nakstad, B, Vatn, MH, Jahnsen, J, Ricanek, P, Vatn, S, Moen, AEF, Tannæs, TM, Lindstrøm, JC, Söderholm, JD, Halfvarson, J, Gomollón, F, Casén, C, Karlsson, MK, Kalla, R, Adams, AT, Satsangi, J, Perminow, G

“…Low Faecalibacterium prausnitzii abundance was associated with prior antibiotic therapy (P=0.001), surgery (P=0.02), and nonmucosal healing (P&lt;0.03). …”