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Automation of the golden gate assembly reaction at low volumes using pin tools
Published 2016Get full text
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Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly.
Published 2018-01-01“…A community-wide standard in DNA assembly would enable bio-molecular engineering at the levels of predictivity and universality in design and construction that are comparable to other engineering fields. Golden Gate Assembly technology, with its robust capability to unidirectionally assemble numerous DNA fragments in a one-tube reaction, has the potential to deliver a universal standard framework for DNA assembly. …”
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METAPHORICS OF THE EXPERIMENTAL THEATER “KYIV GOLDEN GATE THEATER” AS A REFLECTION OF EUROPEAN THEATERICAL POSTMODERNISM
Published 2018-12-01“…The purpose of the article is to define the peculiarities of metaphorical directing of the experimental theater “Kyiv Golden Gate Theater” during the period of 1984–2014. …”
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Sandstone geomorphology of the Golden Gate Highlands National Park, South Africa, in a global context
Published 2011-03-01“…The Golden Gate Highlands National Park (GGHNP) is well known for its impressive sandstone formations. …”
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Golden Gate Cloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors for Plants
Published 2020-10-01“…In this article, we present Golden Gate cloning-compatible modular systems allowing rapid and flexible construction of polycistronic expression vectors applicable for plants. …”
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Sandstone weathering, Electrical Resistivity Tomography, and the deterioration of San Rock Art in the Golden Gate Highlands National Park, South Africa
Published 2011“…</p><p>This research was continued by investigating the role of internal moisture in sandstone weathering using the Golden Gate Highlands National Park (GGHNP), South Africa as a case study. …”
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PluriBAC: A Versatile Baculovirus-Based Modular System to Express Heterologous Genes in Different Biotechnological Platforms
Published 2023-09-01“…The PluriBAC system consists of three insert entry levels using Golden Gate assembly technology. The wide availability of vectors and sticky ends allows enough versatility to combine more than four different promoters, genes of interest, and terminator sequences. …”
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AHEAD: Automatic Holistic Energy-Aware Design Methodology for MLP Neural Network Hardware Generation in Proactive BMI Edge Devices
Published 2020-05-01“…The results show that the proposed methodology is up to a 4X faster in performance, 3X lower in terms of power consumption, and achieves a 5X reduction in area resources, with exact accuracy, compared to floating-point and half-floating-point design on a field-programmable gate array (FPGA), which makes it a promising design methodology for proactive BMI edge devices.…”
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Defeating Untrustworthy Testing Parties: A Novel Hybrid Clustering Ensemble Based Golden Models-Free Hardware Trojan Detection Method
Published 2019-01-01“…Both the EDA evaluation on ISCAS89 benchmarks and field-programmable gate array evaluation on Trust-HUB benchmarks are performed to evaluate the performance of the proposed method. …”
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A Power Transformer Fault Diagnosis Method Based on Improved Sand Cat Swarm Optimization Algorithm and Bidirectional Gated Recurrent Unit
Published 2023-01-01“…The bidirectional gated recurrent unit (BiGRU) method based on dissolved gas analysis (DGA) has been studied in the field of power transformer fault diagnosis. …”
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Two Novel Measurements for the Drive-Mode Resonant Frequency of a Micromachined Vibratory Gyroscope
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FPGA-based fault injection design for 16K-point FFT processor
Published 2019-10-01Subjects: Get full text
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SANParks, people and adaptive management: Understanding a diverse field of practice during changing times
Published 2011-05-01Get full text
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FungalBraid 2.0: expanding the synthetic biology toolbox for the biotechnological exploitation of filamentous fungi
Published 2023-08-01“…Recently, an increasing number of synthetic biology toolkits have been developed and applied to filamentous fungi, which highlights the relevance of these organisms in the biotechnology field. The FungalBraid (FB) modular cloning platform enables interchangeability of DNA parts with the GoldenBraid (GB) platform, which is designed for plants, and other systems that are compatible with the standard Golden Gate cloning and syntax, and uses binary pCAMBIA-derived vectors to allow Agrobacterium tumefaciens-mediated transformation of a wide range of fungal species. …”
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Lo spirito dorato. San Francisco e il Parco Crissy Field degli Hargreaves Associates
Published 2015-11-01“…<div class="page" title="Page 1"><div class="layoutArea"><div class="column"><p><span>Nel saggio si presenta il progetto di riqualificazione degli Hargreaves Associates per il </span><span><em>waterfront</em> </span><span>della costa nord di San Francisco, un’ area dimessa dall’aviazione ed appartenente al Golden Gate National Recreational Area denominata Crissy Field. …”
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Quick identification of ABC trilayer graphene at nanoscale resolution via a near-field optical route
Published 2023-01-01“…Consequently, the IR contrast between ABC and ABA becomes reversed and can even be striking (ABC/ABA ∼ 3) near the graphene phonon frequency. The observed near-field IR features can serve as a golden rule to quickly distinguish ABA and ABC trilayers with no ambiguity, which could largely advance the exploration of correlation physics in ABC-stacked trilayer graphene.…”
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Time-resolved velocity mapping at high magnetic fields: A preclinical comparison between stack‐of‐stars and cartesian 4D-Flow
Published 2022-09-01“…Purpose: Prospectively-gated Cartesian 4D-flow (referred to as Cartesian-4D-flow) imaging suffers from long TE and intensified flow-related intravoxel-dephasing especially in preclinical ultra-high field MRI. …”
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Chinook salmon and green sturgeon migrate through San Francisco Estuary despite large distortions in the local magnetic field produced by bridges.
Published 2017-01-01“…Significant numbers of tagged Chinook salmon smolts migrated past bridges, which produced strong magnetic anomalies, to the Golden Gate Bridge, where they were recorded by dual arrays of acoustic tag-detecting monitors moored in lines across the mouth of the bay. …”
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