Lipid Extraction from HeLa Cells, Quantification of Lipids, Formation of Large Unilamellar Vesicles (LUVs) by Extrusion and in vitro Protein-lipid Binding Assays, Analysis of the I... by Amina Bittame, Jodie Lopez, Gregory Effantin, Nicolas Blanchard, Marie-France Cesbron-Delauw, Jean Gagnon, Corinne Mercier

“…This article describes the protocols for 1) extraction of total lipids from eukaryotic cells using the method developed by Bligh and Dyer (1959), 2) the quantification of glycerophospholipids by gas chromatography after methanolysis, followed by 3) the formation of LUVs by extrusion, 4) protein-lipid binding assay, 5) analysis of the incubation product by transmission electron microscopy (TEM) and by flotation across a discontinuous sucrose gradient and finally, 6) analysis of the proteins by immunoblot and revelation of the glycerophospholipids by iodin fumigation.…”
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Lamellarity-Driven Differences in Surface Structural Features of DPPS Lipids: Spectroscopic, Calorimetric and Computational Study by Lea Pašalić, Barbara Pem, Danijela Bakarić

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Anatomical Variations on NCCT Nose and Paranasal Sinuses and Their Relation with Symptoms of the Patients: A Retrospective Analysis by Riya Thakral, Abhinav Srivastav, Anshul Chatrath, Vishwani Khanna, Sanjeev Awasthi, Vivek Kumar Pathak, Luv Gupta

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Correction: Identification of TRAIL-inducing compounds highlights small molecule ONC201/TIC10 as a unique anti-cancer agent that activates the TRAIL pathway by Joshua E Allen, Gabriel Krigsfeld, Luv Patel, Patrick A Mayes, David T Dicker, Gen Sheng Wu, Wafik S El-Deiry

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Smoking and smoking cessation in relation to risk of diabetes in Chinese men and women: a 9-year prospective study of 0.5 million people by Liu, X, Bragg, F, Yang, L, Kartsonaki, C, Guo, Y, Du, H, Bian, Z, Chen, Y, Yu, C, Luv, J, Wang, K, Zhang, H, Chen, J, Clarke, R, Collins, R, Peto, R, Li, L, Chen, Z

Boosting REsources And caregiver empowerment for Tracheostomy care at HomE (BREATHE) Study: study protocol for a stratified randomization trial by Karen Sepucha, Kevin Callans, Lauren Leavitt, Yuchiao Chang, Ha Vo, Matthew Brigger, Stacey Broughton, Jennifer Cahill, Siva Chinnadurai, Janet Germann, Teresa Giordano, Hanna Greenlick-Michals, Luv Javia, Asitha D. L. Jayawardena, Jeffery Osthimer, Rosemary Chandy Patel, Andrew Redmann, Sergei Roumiantsev, Leigh Simmons, Matthew Smith, Michelle Tate, Mollie Warren, Kimberly Whalen, Phoebe Yager, Habib Zalzal, Christopher Hartnick

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Scleral Buckling for Primary Retinal Detachment: Outcomes of Scleral Tunnels versus Scleral Sutures by Matthew R. Starr, Edwin H. Ryan, Anthony Obeid, Claire Ryan, Xinxiao Gao, Malika L. Madhava, Sean M. Maloney, Adam Z. Adika, Krishi V. Peddada, Kareem Sioufi, Luv G. Patel, Michael J. Ammar, Nora J. Forbes, Antonio Capone Jr., Geoffrey G. Emerson, Daniel P. Joseph, Dean Eliott, Carl D. Regillo, Jason Hsu, Omesh P. Gupta, Yoshihiro Yonekawa, for the Primary Retinal Detachment Outcomes (PRO) Study Group

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A Watershed-Segmentation-Based Improved Algorithm for Extracting Cultivated Land Boundaries by Yongan Xue, Jinling Zhao, Mingmei Zhang

“…The extraction accuracy was compared to the RGB color space using the <i>δ_A</i>, <i>δ_P</i>, and <i>K_hat</i>, that were improved by 76.92%, 62.01%, and 16.83%, respectively, in the Lab color space, while they were 55.79%, 49.67%, and 13.42% in the Luv color space. (4) Through the visual comparison, time efficiency, and segmentation accuracy, the comprehensive extraction effect using the proposed algorithm was obviously better than that of RGB color-based space algorithm. …”
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Measures of star formation rates from infrared (Herschel) and UV (GALEX) emissions of galaxies in the HerMES fields by Buat, V, Giovannoli, E, Burgarella, D, Altieri, B, Amblard, A, Arumugam, V, Aussel, H, Babbedge, T, Blain, A, Bock, J, Boselli, A, Castro-Rodriguez, N, Cava, A, Chanial, P, Clements, D, Conley, A, Conversi, L, Cooray, A, Dowell, C, Dwek, E, Eales, S, Elbaz, D, Fox, M, Franceschini, A, Gear, W

Star forming galaxies at z ≈ 6 and reionization by Bunker, A, Stanway, E, Ellis, R, McMahon, R, Eyles, L, Lacy, M

“…This declining comoving star formation rate (0.005 h70 M⊙ yr-1 Mpc-3 at z ≈ 6 at LUV &gt; 0.1L* for a Salpeter IMF) poses an interesting challenge for models which suggest that LUV &gt; 0.1L* star forming galaxies at z ≃ 6 reionized the universe. …”

ASMUS — Ein numerisches Modell zur Berechnung der Strömung und der Schadstoffverteilung im Bereich einzelner Gebäude. I: Das Strömungsfeld by Günter Gross, Rainer Röckle, Ulrike Janssen

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Further observational evidence for a critical ionizing luminosity in active galaxies by Curran, S, Hunstead, R, Johnston, H, Whiting, M, Sadler, E, Allison, J, Bignell, C

“…Based upon the detection rate for z ≥ 0.1 associated absorption, we would expect approximately four detections. Of the 11 which have previously not been searched, there is sufficient source-frame optical/ultraviolet photometry to determine the ionizing photon rate for four. …”

Atomic and molecular absorption in redshifted radio sources by Curran, SJ, Whiting, MT, Allison, JR, Tanna, A, Sadler, EM, Athreya, R

“…We report on a survey for associated H I 21-cm and OH 18-cm absorption with the Giant Metrewave Radio Telescope at redshifts z ≈ 0.2–0.4. Although the low-redshift selection ensures that our targets are below the critical ultraviolet luminosity (LUV ∼ 1023 W Hz−1), which is hypothesized to ionize all of the neutral gas in the host galaxy, we do not obtain any detection in the six sources searched. …”

Precision diagnosis of burn injuries using imaging and predictive modeling for clinical applications by Pramod K. B. Rangaiah, B P Pradeep kumar, Fredrik Huss, Robin Augustine

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Proteoliposomes for Studying Lipid-protein Interactions in Membranes in vitro by Helmut Kirchhoff

“…This protocol describes the isolation of LHCII from leaves and details a four-step procedure to incorporate the detergent-solubilized membrane protein in large unilamellar vesicles (LUV). …”
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Neutrino nonstandard interactions and lepton flavor universality violation at SND@LHC via charm production by Bhubanjyoti Bhattacharya, Alakabha Datta, Elena Graverini, Lopamudra Mukherjee, Divya Sachdeva, John Waite

“…Finally, we also study the effects of lepton universality violation (LUV) assuming that the new physics coupling is not lepton universal.…”
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Obscured star formation in bright z ≃ 7 Lyman-break galaxies by Bowler, R, Bourne, N, Dunlop, J, McLure, R, McLeod, D

The strong gravitationally lensed Herschel galaxy HLock01: Optical spectroscopy reveals a close galaxy merger with evidence of inflowing gas by Marques-Chaves, R, Pérez-Fournon, I, Gavazzi, R, Martínez-Navajas, P, Riechers, D, Rigopoulou, D, Cabrera-Lavers, A, Clements, D, Cooray, A, Farrah, D, Ivison, R, Jiménez-Ángel, C, Nayyeri, H, Oliver, S, Omont, A, Scott, D, Shu, Y, Wardlow, J

“…While the SMG appears very massive (M∗≃ 5 ×1011Mo), with a highly extinguished stellar component (AV≃ 4.3), the LBG is a young, lower-mass (M∗≃ 1 ×1010Mo), but still luminous (10 × LUV∗) satellite galaxy. …”

Adduction of cholesterol 5,6-secosterol aldehyde to membrane-bound myelin basic protein exposes an immunodominant epitope. by Cygan, N, Scheinost, J, Butters, T, Wentworth, P

Hubble Space Telescope imaging and keck spectroscopy of z approximate to 6 i-band dropout galaxies in the Advanced Camera for Surveys GOODS fields by Stanway, E, Bunker, A, McMahon, R, Ellis, R, Treu, T, McCarthy, P

“…Using the rest-frame UV continuum from our six candidate z ∼ 6 galaxies from the GOODS-N field, we determine a lower limit to the unobscured volume-averaged global star formation rate at z ∼ 6 of (5.4 ± 2.2) × 10 -4 h70 M⊙ yr-1 Mpc-3. We find that the cosmic star formation density in Lyman break galaxies (LBGs) with unobscured star formation rates greater than 15 M⊙ yr-1 falls by a factor of 8 between z ∼ 3 and z ∼ 6. …”