Development of a Comparative European Orthohantavirus Microneutralization Assay With Multi- Species Validation and Evaluation in a Human Diagnostic Cohort by Tabitha E. Hoornweg, Ilse Zutt, Ankje de Vries, Miriam Maas, Marieke N. Hoogerwerf, Tatjana Avšič-Županc, Miša Korva, Johan H. J. Reimerink, Chantal B. E. M. Reusken

“…The assay was validated using RT-PCR-confirmed rodent (n=17) and human sera (n=17), DOBV-suspected human sera (n=3) and cohorts of orthohantavirus-negative rodent (n=3) and human sera (n=85). 16/17 RT-PCR-confirmed rodent sera and 18/20 of the RT-PCR-confirmed and DOBV-suspected human sera were serotyped successfully, while for the remaining rodent (n=1) and human sera (n=2) no neutralizing titers could be detected. …”
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A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus by Carlos Fernando Odir Rodrigues Melo, Luiz Claudio Navarro, Diogo Noin de Oliveira, Tatiane Melina Guerreiro, Estela de Oliveira Lima, Jeany Delafiori, Mohamed Ziad Dabaja, Marta da Silva Ribeiro, Maico de Menezes, Rafael Gustavo Martins Rodrigues, Karen Noda Morishita, Cibele Zanardi Esteves, Aline Lopes Lucas de Amorim, Caroline Tiemi Aoyagui, Pierina Lorencini Parise, Guilherme Paier Milanez, Gabriela Mansano do Nascimento, André Ricardo Ribas Freitas, André Ricardo Ribas Freitas, Rodrigo Angerami, Fábio Trindade Maranhão Costa, Clarice Weis Arns, Mariangela Ribeiro Resende, Eliana Amaral, Renato Passini Junior, Carolina C. Ribeiro-do-Valle, Helaine Milanez, Maria Luiza Moretti, Jose Luiz Proenca-Modena, Sandra Avila, Anderson Rocha, Rodrigo Ramos Catharino

“…Recent Zika outbreaks in South America, accompanied by unexpectedly severe clinical complications have brought much interest in fast and reliable screening methods for ZIKV (Zika virus) identification. …”
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Identification of CRF66_BF, a New HIV-1 Circulating Recombinant Form of South American Origin by Joan Bacqué, Elena Delgado, Sonia Benito, María Moreno-Lorenzo, Vanessa Montero, Horacio Gil, Mónica Sánchez, María Carmen Nieto-Toboso, Josefa Muñoz, Miren Z. Zubero-Sulibarria, Estíbaliz Ugalde, Elena García-Bodas, Javier E. Cañada, Jorge del Romero, Carmen Rodríguez, Iciar Rodríguez-Avial, Luis Elorduy-Otazua, José J. Portu, Juan García-Costa, Antonio Ocampo, Jorge J. Cabrera, Michael M. Thomson

“…Through a Bayesian coalescent analysis, the most recent common ancestor of CRF66_BF was estimated around 1984 in South America, either in Paraguay or Argentina. Among Pr-RT sequences obtained by us from HIV-1-infected Paraguayans living in Spain, 14 (20.9%) of 67 were of CRF66_BF, suggesting that CRF66_BF may be one of the major HIV-1 genetic forms circulating in Paraguay. …”
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Insights into the Host Range, Genetic Diversity, and Geographical Distribution of Jingmenviruses by Sarah Temmam, Thomas Bigot, Delphine Chrétien, Mathilde Gondard, Philippe Pérot, Virginie Pommelet, Evelyne Dufour, Stéphane Petres, Elodie Devillers, Thavry Hoem, Valérie Pinarello, Vibol Hul, Khamsing Vongphayloth, Jeffrey C. Hertz, Irène Loiseau, Marine Dumarest, Veasna Duong, Muriel Vayssier-Taussat, Marc Grandadam, Emmanuel Albina, Philippe Dussart, Sara Moutailler, Julien Cappelle, Paul T. Brey, Marc Eloit

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COVID-19, SARS-CoV-2, influenza, adenovirus, pneumonia, comparison by Esma KEPENEK KURT, Bahar KANDEMİR, İbrahim ERAYMAN, Hülya VATANSEV, Adil ZAMANİ, Şebnem YOSUNKAYA, Soner DEMİRBAŞ, Celalettin KORKMAZ, Pınar Diydem YILMAZ, Necdet POYRAZ, Bahadır FEYZİOĞLU, Mehmet ÖZDEMİR, Mehmet UYAR, Tevfik KÜÇÜKKARTALLAR

“…Results: Among 237 RT-PCR positive patients, 104 (43.9%) were female and 133 (56.1%) were male. …”
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Diagnosis of hantavirus infection in humans and rodents in Ribeirão Preto, State of São Paulo, Brazil Diagnóstico de infecção por hantavírus em humanos e roedores em Ribeirão Preto... by Glauciane Garcia de Figueiredo, Alessandra Abel Borges, Gelse Mazzoni Campos, Alex Martins Machado, Fabiano Pinto Saggioro, Gilberto dos Santos Sabino Júnior, Soraya Jabur Badra, Alberto Anastacio Amarilla Ortiz, Luiz Tadeu Moraes Figueiredo

“…METHODS: HPCS was diagnosed by means of ELISA and/or RT-PCR in 11 (21.2%) out of 52 suspected cases, and 54.4% of these were fatal. …”
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Comparison of Demographic Profile and CT Findings in COVID-19 Pneumonia between First and Second Waves of COVID-19 Pandemic: A Retrospective Study by Taling Mari, Wangju Sumnyan, Kenjum Riba, Nyapu Soki

“…The demographic profile, radiological pattern based on the Radiological Society of North America (RSNA) consensus statement and CT severity index in COVID-19 pneumonia were compared during the peak three months of first and second waves of the pandemic. …”
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Molecular and genetic characteristics of the multicomponent flavi-like Kindia tick virus (Flaviviridae) found in ixodes ticks on the territory of the Republic of Guinea by Mikhail Yu. Kartashov, Anastasia V. Gladysheva, Ekaterina V. Naidenova, Kirill S. Zakharov, Аlexander N. Shvalov, Ekaterina I. Krivosheina, Aislu M. Senichkina, Mamadou B. Bah, Vladimir A. Ternovoi, Sanaba Boumbaly, Valery B. Loktev

“…The detection of viral RNA was carried out in individual samples of ticks by RT-PCR, followed by the determination of the nucleotide sequence and phylogenetic analysis. …”
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Detection of rabies virus in organs unrelated to the central nervous system of experimentally-inoculated vampire bats by María Luisa Méndez Ojeda, Edith Rojas Anaya, José Francisco Morales Álvarez, Graciela Tapia Pérez, Gerardo Suzán, Osiris Gaona Pineda, Rodrigo A. Medellín Legorreta, Charles E Rupprecht, Elizabeth Loza-Rubio

“…Viral RNA was detected in brain, salivary gland and tongue tissue by RT-PCR and nested PCR (nPCR). Viral genome was also detected in organs unrelated to the central nervous system. …”
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Ethnographic Study at a Music Library Found Students Prefer Short Stopovers and Longer Solitary Study by Dominique Daniel

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A Newly Emerging Serotype A Strain in Foot-and-Mouth Disease Virus with Higher Severity and Mortality in Buffalo than in Cattle Calves in North Egypt by Samy Metwally, Nabil Bkear, Yassien Badr, Besheer Elshafey, Sadeq K. Alhag, Laila A. Al-Shuraym, Gaber Batiha, Bassant Fakhry, Rania Hamada

“…A FMDV genome was detected and serotyped using one-step reverse transcription polymerase chain reactions (RT-PCRs). Nucleotide sequencing of the purified serotype-specific PCR bands was performed, and a maximum likelihood phylogenetic tree based on 600 base pairs of VP1 was constructed. …”
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Genome-wide characterization and expression profiling of NAC transcription factor genes under abiotic stresses in radish (Raphanus sativus L.) by Bernard Kinuthia Karanja, Liang Xu, Yan Wang, Everlyne M’mbone Muleke, Bashir Mohammed Jabir, Yang Xie, Xianwen Zhu, Wanwan Cheng, Liwang Liu

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Low prevalence of hepatitis delta infection in Cuban HBsAg carriers: Prospect for elimination by Licel de los Ángeles Rodríguez Lay, Zexi Tan, Maria Caridad Montalvo Villalba, Marcia Samada Suárez, Marité Bello Corredor, Dayesi López Hernández, Barbara Marrero Sánchez, Lidunka Valdés Alonso, Aurélie Sausy, Judith M. Hübschen

“…If positive, the samples were analyzed for HDV-RNA using Real-Time RT-PCR targeting the ribozyme and HD antigen domains followed by genotyping based on phylogenetic analysis.ResultsTwo samples were anti-HDV positive [0.39% (95% CI 0.11–1.44)]. …”
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Occurrence of SARS-CoV-2 reinfections at regular intervals in Ecuador by Rommel Guevara, Belén Prado-Vivar, Sully Márquez, Erika B. Muñoz, Mateo Carvajal, Juan José Guadalupe, Mónica Becerra-Wong, Stefanie Proaño, Rosa Bayas-Rea, Josefina Coloma, Michelle Grunauer, Gabriel Trueba, Patricio Rojas-Silva, Verónica Barragán, Paúl Cárdenas

“…Suspected reinfections were identified retrospectively from a database of RT-qPCR-positive patients. Cases were confirmed by sequencing viral genomes from the first and second infections using the ONT MinION platform. …”
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Global epidemiology of drug resistance after failure of WHO recommended first-line regimens for adult HIV-1 infection: a multicentre retrospective cohort study by Gregson, J, Tang, M, Ndembi, N, Hamers, R, Marconi, V, Brooks, K, Theys, K, Arruda, M, Garcia, F, Monge, S, Kanki, P, Kumarasamy, N, Kerschberger, B, Mor, O, Charpentier, C, Todesco, E, Rokx, C, Gras, L, Halvas, E, Sunpath, H, Carlo, D, Antinori, A, Andreoni, M, Latini, A, Mussini, C, Aghokeng, A, Sonnerborg, A, Neogi, U, Fessel, W, Agolory, S, Yang, C, Blanco, J, Juma, J, Smit, E, Schmidt, D, Watera, C, Asio, J, Kirungi, W, Tostevin, A, Clumeck, N, Goedhals, D, Bester, P, Sabin, C, Mukui, I, Santoro, M, Perno, C, Hunt, G, Morris, L, Camacho, R, Pillay, D, Schulter, E, Reyes-Terán, G, Romero, K, Avila-Rios, S, Sirivichayakul, S, Ruxrungtham, K, Mekprasan, S, Dunn, D, Kaleebu, P, Raizes, E, Kantor, R, Shafer, R, Gupta, R, Rhee, S, De Wit, T, Diero, L, Günthard, H, Hoffmann, C, Di Carlo, D

“…Our primary outcome was tenofovir resistance, defined as presence of K65R/N or K70E/G/Q mutations in the reverse transcriptase (RT) gene. Findings We included 1926 patients from 36 countries with treatment failure between 1998 and 2015. …”

Atypical Porcine Pestivirus as a Novel Type of Pestivirus in Pigs in China by Jin Yuan, Zhiyong Han, Jun Li, Yunzhen Huang, Jiongfeng Yang, Hongxing Ding, Jingyuan Zhang, Mengjiao Zhu, Yangyi Zhang, Jiedan Liao, Mingqiu Zhao, Jinding Chen

“…Recently, a novel atypical porcine pestivirus (APPV) linked with the development of congenital tremor (CT) in neonatal pigs was described in Europe and the Americas. Here, the first Asian APPV complete polyprotein coding sequence was assembled from serum samples from newborn piglets affected with CT in Southern China, and termed APPV_GD. 14 organ samples from affected piglets were analyzed by quantitative RT-PCR (qRT-PCR) to investigate the tissue tropism of APPV, and 135 serum samples from pigs from 10 farms were used for identifying APPV in adult pigs. …”
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Epidemiological and viral features of a cohort of SARS-CoV-2 symptomatic and asymptomatic individuals in an area of the Colombian Caribbean by Salim Mattar, Caty Martinez-Bravo, Ricardo Rivero, Héctor Contreras, Álvaro A. Faccini-Martínez, Camilo Guzman-Teran, Ketty Galeano, Nelson Alvis-Guzman, Verónica Contreras, German Arrieta, Marco Gonzalez-Tous, Jorge Miranda, Martha Ospina, Francisco Camargo-Assis, Marcela Mercado-Reyes, Evelyn Garay, Alejandra Garcia-Perez, Yesica Lopez, Vaneza Tique

“…As other world regions, South America has not contained the pandemic’s advance since it lacks the hospital and economic capacities. …”
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Emergence and dissemination of equine-like G3P[8] rotavirus A in Brazil between 2015 and 2021 by Meylin Bautista Gutierrez, Ighor Arantes, Gonzalo Bello, Lúcia Helena Berto, Leonardo Hermes Dutra, Rodrigo Bentes Kato, Tulio Machado Fumian

“…To understand its global emergence and dissemination in Brazilian territory, stool samples collected from 11 Brazilian states (n = 919) were genotyped by RT-qPCR and proceeded to sequence the VP7 gene (n = 102, 79 being newly generated) of the G3P[8] samples with pronounced viral loads. …”
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Tracing the evolutionary history of hepatitis B virus genotype H endemic to Mexico by Alexis Jose-Abrego, Alexis Jose-Abrego, Sonia Roman, Sonia Roman, Saul Laguna-Meraz, Saul Laguna-Meraz, Saul Laguna-Meraz, João Renato Rebello-Pinho, João Renato Rebello-Pinho, Santiago Justo Arevalo, Santiago Justo Arevalo, Arturo Panduro, Arturo Panduro

“…Ninety-two HBV sequences of the reverse transcriptase (RT) domain of the polymerase gene (~1,251 bp) were analyzed; 48 were genotype H, 43 were genotype F, and the oldest HBV sequence from America was included as the root. …”
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Clinical Profile of SARS-CoV-2 Infection: Mechanisms of the Cellular Immune Response and Immunogenetic Markers in Patients from Brazil by Vanessa Pacheco, Rosane Cuber Guimarães, Danielly Corrêa-Moreira, Carlos Eduardo Magalhães, Douglas Figueiredo, Patricia Guttmann, Gisela Freitas Trindade, Juliana Fernandes Amorim da Silva, Ana Paula Dinis Ano Bom, Maria de Lourdes Maia, Juliana Gil Melgaço, Tamiris Azamor da Costa Barros, Andrea Marques Vieira da Silva, Collaborative Group, Manoel Marques Evangelista Oliveira

“…Methods: This is a cohort study of 70 cases of COVID-19, confirmed by RT-PCR, followed up to 60 days. Plasma Samples and clinical data were. …”
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