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DIFFERENTIATED PRE-ADIPOCYTES ACT AS A DRIVING FORCE FOR PROSTATE CANCER PROLIFERATION, MIGRATION AND EPITHELIAL-MESENCHYMAL TRANSITION
Published 2022-04-01“…In the current study, condition media (CM) obtained from differentiated pre-adipocytes was used to set an indirect co-culture system with the prostate cancer cell line. Firstly, adipogenesis of 3T3F44-2A was checked by oil red o staining and expressions of specific genes. …”
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Chemical Constituents and Biological Activities of Garcinia Cuneifolia, Mesua Beccariana and Mesua Ferrea
Published 2005“…The main essential oil component is germacrene D (48.96%). Both the crude extracts of G. cuneifolia and M. beccariana (stem bark) were not active towards CEM-SS cells line, except for the hexane extract of M. beccariana, which showed weak cytotoxic effect. …”
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Novel topical nano-colloidal carrier loaded with cyclosporine: biological evaluation potentially for psoriasis treatment
Published 2021“…Psoriasis is an autoimmune condition appeared as a skin disease, spotted as dry skin with red and thick plagues, which commonly induce itchiness and extreme uncomfortable feeling to the patient. …”
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Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution
Published 2013-08-01“…The cells were differentiated into adipocytes for 5 days with a standard procedure, stained with Oil Red O and observed with an optical microscope. …”
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Spermine Suppresses Adipocyte Differentiation and Exerts Anti-Obesity Effects In Vitro and In Vivo
Published 2022-10-01“…The preadipocyte cell line, 3T3-L1, was cultured with Put, Spd, or Spm, and lipid accumulation in the cells was measured by Oil Red O staining. …”
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Prostaglandin F2α Regulates Adipogenesis by Modulating Extracellular Signal-Regulated Kinase Signaling in Graves’ Ophthalmopathy
Published 2023-04-01“…We used Ebopiprant (FPR antagonist) to interfere with PGF2α binding to the FPR and U0126, an Extracellular Signal-Regulated Kinase (ERK) inhibitor, to inhibit ERK phosphorylation. The results of Oil red O staining and expression of adipogenic markers showed that blocking the receptor binding or decreasing the phosphorylation state of the ERK both alleviate the inhibitory effect of PGF2a on the OFs adipogenesis. …”
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Antifibrotic Effect of Transforming Growth Factor Beta 1 Inhibitor Extract from Streptomyces SP.Strain H6552 on Human Hepatic Stellate Cells
Published 2010“…In HSC culture studies, LX-2 cells were seeded either on non-coated or Matrigel-coated culture plates and subjected to fibrotic marker analyses, Oil Red O staining, and phase contrast microscopy. …”
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Nickel-Catalyzed Enantioselective Negishi Cross-Couplings of Racemic Secondary alpha-Bromo Amides with Alkylzinc Reagents: (S)-N-Benzyl-7-cyano-2-ethyl-N-phenylheptanamide
Published 2011“…The neck is re-capped with a rubber septum, and the reaction mixture is stirred at 70 °C in an oil bath until the red color fades (~5 min). 6-Bromohexanenitrile (13.2 mL, 100 mmol, 1.00 equiv) (Note 4) is added via syringe over 4 min, and the reaction mixture is stirred at 70 °C for 12 h. …”
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Dual Beneficial Effects of Methylnissolin-3-O-β-<span style="font-variant: small-caps">d</span>-Glucopyranoside on Obesity-Induced Inflammatory Responses in Adipocyte-Macrophage Co...
Published 2022-06-01“…Intracellular lipid accumulation was determined using Oil Red O staining. Western blotting was performed to investigate the expression of adipogenesis- and inflammation-associated proteins. …”
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Bioavailability and Pharmacokinetics Studies of Gamma Oryzanol
Published 2004“…By day 9, cell line showed polarized monolayer properties as was detected from transepithelial electrical resistance (TEER) value (247.2 * 25.0 &m2) and phenol red diffusion (4.2 + 0.1 %). …”
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Balancing Biodiversity with Land Use in the Lowland Rainforests of Peninsular Malaysia, a Discussion Paper
Published 2006“…Among a total of 500 terrestrial species of birds (including inland species that occupy riparian habitats) found in Peninsular Malaysia, 156 are endemic to the Sundaland (Peninsular Malaysia, Sumatra, Java, Bali, Borneo and smaller island west of the Wallace Line) of which 82 (53%) are now red-listed by IUCN. …”
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Poly (Lactic-<i>co</i>-Glycolic Acid) Nanoparticles and Nanoliposomes for Protein Delivery in Targeted Therapy: A Comparative <i>In</i> <i>Vitro</i> Study
Published 2020-11-01“…In order to explore a possible application of these nanocarriers for a targeted therapy in the cardiovascular field, hemolytic activity and biocompatibility, in terms of cell viability, were performed by using human red blood cells and EA.hy926 human endothelial cell line, respectively.…”
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FHL2 regulates THP-1 macrophage foaming through NF-κB signaling pathway
Published 2024-02-01“…The cells were stimulated with oxidized low density lipoprotein(ox-LDL) and the expression of IL-6, IL-1β,TNF-α and other cytokines were detected by ELISA. Oil red O staining was used to detect the degree of cell foaming. …”
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The effect of hypoxia on myogenic differentiation and multipotency of the skeletal muscle-derived stem cells in mice
Published 2022-02-01“…Hypoxia promoted both SC adipogenesis and osteogenesis under respective induction as shown by using Oil Red O and Alizarin Red S staining. The expression of adipogenic markers; peroxisome proliferator activated receptor gamma (PPARγ) and fatty acid-binding protein 4 (FABP4) were upregulated under hypoxia up to day 14 compared to normoxic condition. …”
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Exosomes biogenesis was increased in metformin-treated human ovary cancer cells; possibly to mediate resistance
Published 2024-04-01“…Metformin increased the activity of caspase-3 (P < 0.05) as well as the number of Oil-Red-O positive cells in both cell lines. In vitro scratch assay showed that the cell migration rate of metformin-treated cells was decreased (P < 0.05), whereas AChE activity of exosomes from metformin-treated cells was increased (P < 0.05). …”
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Chronic Exposure to Low-Molecular-Weight Polycyclic Aromatic Hydrocarbons Promotes Lipid Accumulation and Metabolic Inflammation
Published 2023-01-01“…In consistence, an increase in lipid accumulation was demonstrated in BODIPY and Oil Red O-stained adipocytes. Additionally, 3T3-L1 adipocytes and RAW264.7 macrophages chronically exposed to 2-naphthol showed upregulated mRNA expression of major inflammatory cytokines (e.g., tumor necrosis factor α (<i>Tnfα),</i> interleukin-1β <i>(Il-1β), <i>and</i> Il-6)</i>. …”
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Pharmaceutical significance of Leuconostoc mesenteroides KS-TN11 isolated from Nile Tilapia, Oreochromis niloticus
Published 2018-05-01“…Effect of KS-TN11 on lipid accumulation in adipocytes was done by using Oil Red O staining. The isolate showed strong antibacterial activity against a number of pathogenic bacteria in vitro. …”
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Study on Carbohydrate Metabolism in Adult Zebrafish (Danio rerio)
Published 2023-01-01“…Oil red O staining, triglyceride content, and Hematoxylin-Eosin staining results showed that dietary dextrin levels of ≥30% significantly increased lipid accumulation and liver damage, as well as processes related to glycolipid metabolism and inflammation in zebrafish. …”
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