Cell adhesion to agrin presented as a nanopatterned substrate is consistent with an interaction with the extracellular matrix and not transmembrane adhesion molecules by Wolfram Tobias, Spatz Joachim P, Burgess Robert W

“…This has been demonstrated using nanopatterned RGD peptides, a canonical extracellular matrix ligand for integrin interactions. Cell adhesion was greatly reduced when the RGD-coated nanoparticles were separated by more than 60 nm, indicating a sharp spacing-dependent threshold for this form of cell adhesion.…”
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Immune responses in advanced colorectal cancer following repeated intradermal vaccination with the anti-CEA murine monoclonal antibody, PR1A3: results of a phase I study. by Zbar, A, Thomas, H, Wilkinson, R, Wadhwa, M, Syrigos, K, Ross, E, Dilger, P, Allen-Mersh, T, Kmiot, W, Epenetos, A, Snary, D, Bodmer, W

“…In vitro PBMC proliferation with mitogens and recall antigens is greatly increased during the course of immunisation, with a shift in stimulated cytokine profile.…”

Research on the anti-ageing mechanism of Prunella vulgaris L. by Ping Li, Xiao Lv, Junrong Wang, Chenyang Zhang, Jiahao Zhao, Yadong Yang

“…These changes rarely present in young tissues, while greatly increasing in response to ageing. Firstly, the ageing model of the Institute of Cancer Research (ICR) mouse was established by d-galactose subcutaneous injection. …”
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ROS generation via NOX4 and its utility in the cytological diagnosis of urothelial carcinoma of the urinary bladder by Fujimoto Kiyohide, Anai Satoshi, Fujii Tomomi, Shimada Keiji, Konishi Noboru

“…As a result, the sensitivity for detection of low grade, non-invasive UC was greatly increased (35% in conventional cytology (C-C) vs. 75% in ROS-C), and the specificity was 95%. …”
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Effect and Mechanism of Andrias davidianus Peptide Supplementation Combined with Treadmill Exercise on Body Mass Loss in Obese Rats by QIAN Jin, FANG Shuangxi, HU Yating, MA Kun, LIU Hui, JIA Shaohui

“…Western blot was used to detect the expression of appetite-regulated proteins (NPY and proopiomelanocortin (POMC)) and energy metabolism-related proteins. …”
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Habitat Imaging of Tumors Enables High Confidence Sub-Regional Assessment of Response to Therapy by Paul David Tar, Neil A. Thacker, Muhammad Babur, Grazyna Lipowska-Bhalla, Susan Cheung, Ross A. Little, Kaye J. Williams, James P. B. O’Connor

“…Our data showed that LPM greatly increased the amount of information extracted from diffusion-weighted imaging, compared to cohort <i>t</i>-tests. …”
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Cellular environment of TTR deposits in an animal model of ATTR—Cardiomyopathy by Cristina Teixeira, Cristina Teixeira, Helena Sofia Martins, Helena Sofia Martins, Maria João Saraiva, Maria João Saraiva

“…It is believed the number of cases to be greatly underestimated mostly due to misdiagnosis. …”
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Illuminating and Radiosensitizing Tumors with 2DG-Bound Gold-Based Nanomedicine for Targeted CT Imaging and Therapy by Maharajan Sivasubramanian, Chia-Hui Chu, Yu Hsia, Nai-Tzu Chen, Meng-Ting Cai, Lih Shin Tew, Yao-Chen Chuang, Chin-Tu Chen, Bulent Aydogan, Lun-De Liao, Leu-Wei Lo

“…Results from this work indicate that 2DG-PEG-AuD could greatly potentiate theranostic capabilities by providing high-resolution anatomical and functional images in a single CT scan and therapeutic capability.…”
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Electrospun sandwich mesh structures loaded with naringenin and vitamin K2 polycaprolactone/gelatin nanofibers synergistically promote bone regeneration by Jiafeng Wang, Longhui Shao, Xiaoyu Wu, Chun Liu, Su Ni, Ting Dai, Hongwei Liu, Hongbin Zhao

“…The biocompatibility and osteogenic capacity of the membranes were assessed in vitro using mouse bone marrow mesenchymal stem cells (BMSCs). …”
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Mannose enhances intestinal immune barrier function and dextran sulfate sodium salt-induced colitis in mice by regulating intestinal microbiota by Yi Yang, Qiming Ma, Qingyu Wang, Lifeng Zhao, Hengshan Liu, Yanjun Chen

“…BackgroundInflammatory bowel disease (IBD) greatly affects human quality of life. Mannose has been reported to be used to treat IBD, but the mechanism is currently unknown.MethodsC57/BL mice were used as research subjects, and the mouse acute colitis model was induced using dextran sulfate sodium salt (DSS). …”
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Gi- and Gq-coupled ADP (P2Y) receptors act in opposition to modulate nociceptive signaling and inflammatory pain behavior by Molliver Derek C, Malin Sacha A

“…</p> <p>Results</p> <p>We detected mRNA and protein for ADP receptors P2Y12 and P2Y13 in mouse dorsal root ganglia (DRG). …”
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Highly efficacious and safe neutralizing DNA aptamer of SARS-CoV-2 as an emerging therapy for COVID-19 disease by Mohamad Ammar Ayass, Trivendra Tripathi, Natalya Griko, Victor Pashkov, Jun Dai, Jin Zhang, Fabian C. Herbert, Ramya Ramankutty Nair, Tutku Okyay, Kevin Zhu, Jeremiah J. Gassensmith, Lina Abi-Mosleh

“…In addition, AYA2012004_L did not induce inflammatory response in the lungs of Th1 and Th2 mouse models as well as human PBMCs. AYA2012004_L was detectable in mice lungs and noticeable in insignificant amounts in other vital organs. …”
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miR‐29a‐3p promotes the regulatory role of eicosapentaenoic acid in the NLRP3 inflammasome and autophagy in microglial cells by Jian‐Ping Pan, Jia‐Li Xie, Li‐Yun Huang, Qi‐Zhen Wu, Dan‐Feng Tang, Qi Jin, Wei Wang, Ming‐Fu Yang

“…Methylated miR‐29a‐3p was detected via methylation‐specific polymerase chain reaction. …”
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Generalised deep learning model for semi-automated length measurement of fish in stereo-BRUVS by Daniel Marrable, Sawitchaya Tippaya, Kathryn Barker, Euan Harvey, Stacy L. Bierwagen, Mathew Wyatt, Scott Bainbridge, Marcus Stowar

“…The head-to-tail measurement method presented here builds on, and advances, previously published object detection for stereo-BRUVS. Furthermore, by replacing the manual process of four careful mouse clicks on the screen to precisely locate the head and tail of a fish in two images, with two fast clicks anywhere on that fish in those two images, a significant reduction in image processing and analysis time is expected. …”
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METTL3 regulates N6-methyladenosine modification of ANGPTL3 mRNA and potentiates malignant progression of stomach adenocarcinoma by Zhijin Zhang, Jun Fu, Yuhao Zhang, Xianju Qin, Yuexia Wang, Chungen Xing

“…Abstract Background N6-methyladenosine (m6A) is associated with mammalian mRNA biogenesis, decay, translation and metabolism, and also contributes greatly to gastrointestinal tumor formation and development. …”
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Comparative transcriptomic analysis of Illumina and MGI next-generation sequencing platforms using RUNX3- and ZBTB46-instructed embryonic stem cells by Szilárd Póliska, Chahra Fareh, Chahra Fareh, Adél Lengyel, Adél Lengyel, Loránd Göczi, Loránd Göczi, József Tőzsér, Istvan Szatmari

“…For example, we found that Gzmb, Gzmd, Gzme, Gdf6, and Ccr7 genes were robustly upregulated upon the forced expression of Runx3; on the other hand, Gpx2, Tdpoz4, and Arg2 were induced alongside the ectopic expression of Zbtb46.Discussion: Similar gene expression profile and greatly overlapping RUNX3- and ZBTB46-regulated gene sets were detected with both DNA sequencing platforms. …”
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Characterisation of AEBP2: a polycomb repressive complex 2 component by Grijzenhout, A

“…Interestingly, I did not detect any peptides of the Polycomblike (PCL) family proteins. …”

Fusion Protein Vaccine Based on Ag85B and STEAP1 Induces a Protective Immune Response against Prostate Cancer by Linpei Guo, Hui Xie, Zheng Zhang, Zhun Wang, Shuanghe Peng, Yuanjie Niu, Zhiqun Shang

“…The uptake of the fusion protein vaccine by DCs was evaluated by confocal microscopy, and DC markers were detected using flow cytometry after incubation with the fusion protein. …”
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Hyperactivation of mTORC1 in a double hit mutant zebrafish model of tuberous sclerosis complex causes increased seizure susceptibility and neurodevelopmental abnormalities by Ann-Sofie De Meulemeester, Lise Heylen, Aleksandra Siekierska, James D. Mills, James D. Mills, James D. Mills, Alessia Romagnolo, Nicole N. Van Der Wel, Eleonora Aronica, Eleonora Aronica, Peter A. M. de Witte

“…Recently, second hit mutations have been detected frequently in mTORopathies. To investigate the pathophysiological effects of second hit mutations, several mouse models have been developed. …”
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Loss of Myostatin Alters Mitochondrial Oxidative Phosphorylation, TCA Cycle Activity, and ATP Production in Skeletal Muscle by Xueqiao Wang, Zhuying Wei, Mingjuan Gu, Lin Zhu, Chao Hai, Anqi Di, Di Wu, Chunling Bai, Guanghua Su, Xuefei Liu, Lei Yang, Guangpeng Li

“…Expectedly, the MSTN null (<i>Mstn</i>^−/−) mouse has a hypermuscular phenotype. The muscle metabolism of the <i>Mstn^−/−</i> mice was detected by an enzyme-linked immunosorbent assay, indirect calorimetry, ChIP-qPCR, and RT-qPCR. …”
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