Simultaneous visualization of RNA transcripts and proteins in whole-mount mouse preimplantation embryos using single-molecule fluorescence in situ hybridization and immunofluoresce... by Rasmani Hazra, David L. Spector

“…Here, we describe a step-by-step, optimized, and robust protocol that allows detection of multiple RNA transcripts and protein molecules in whole-mount preimplantation mouse embryos. …”
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The Time-Feature of Uric Acid Excretion in Hyperuricemia Mice Induced by Potassium Oxonate and Adenine by Shaoshi Wen, Dan Wang, Haiyang Yu, Mengyang Liu, Qian Chen, Ruixia Bao, Lin Liu, Yi Zhang, Tao Wang

“…Hematoxylin-eosin staining was applied to observe kidney pathological changes, and Western blot was used to detect renal urate transporters’ expression. In hyperuricemia mice, plasma uric acid level increased significantly from the 3rd day, and tended to be stable from the 7th day, and the clearance rate of uric acid decreased greatly from the 3rd day. …”
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The calcium channel Orai1 is required for osteoblast development: Studies in a chimeric mouse with variable in vivo Runx-cre deletion of Orai-1. by Lisa J Robinson, Jonathan Soboloff, Irina L Tourkova, Quitterie C Larrouture, Kelechi M Onwuka, Dionysios J Papachristou, Scott Gross, Robert Hooper, Elsie Samakai, Paul F Worley, Peng Liu, Jan Tuckermann, Michelle R Witt, Harry C Blair

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Scleroderma-like Impairment in the Network of Telocytes/CD34⁺ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis by Irene Rosa, Eloisa Romano, Bianca Saveria Fioretto, Daniele Guasti, Lidia Ibba-Manneschi, Marco Matucci-Cerinic, Mirko Manetti

“…Ultrastructural signs of TC injury were detected in the skin of bleomycin-treated mice by TEM. …”
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Multiplex optical urinalysis for early detection of drug-induced kidney injury by Cheng, Penghui, Miao, Qingqing, Huang, Jiaguo, Li, Jingchao, Pu, Kanyi

“…Therefore, MURs hold great promise for detection of kidney function in both preclinical drug screening and clinical settings.…”
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Paxillin interactome identified by SILAC and label-free approaches coupled to TurboID sheds light on the compositions of focal adhesions in mouse embryonic stem cells by He, Qianqian, Sze, Siu Kwan, Ng, Kai Soon, Koh, Cheng-Gee

“…Self-renewal and differentiation of mouse embryonic stem cells (mESCs) are greatly affected by the extracellular matrix (ECM) environment; the composition and stiffness of which are sensed by the cells via integrin-associated focal adhesions (FAs) which link the cells to the ECM. …”
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Engineering of near IR fluorescent albumin nanoparticles for in vivo detection of colon cancer by Cohen Sarit, Margel Shlomo

“…<p>Abstract</p> <p>Background</p> <p>The use of near-infrared (NIR) fluorescence imaging techniques has gained great interest for early detection of cancer because water and other intrinsic biomolecules display negligible absorption or autofluorescence in this region. …”
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scRNA-seq reveals that origin recognition complex subunit 6 regulates mouse spermatogonial cell proliferation and apoptosis via activation of Wnt/β-catenin signaling by Shi-Wei Liu, Jia-Qiang Luo, Liang-Yu Zhao, Ning-Jing Ou, Chao-Yang, Yu-Xiang Zhang, Hao-Wei Bai, Hong-Fang Sun, Jian-Xiong Zhang, Chen-Cheng Yao, Peng Li, Ru-Hui Tian, Zheng Li, Zi-Jue Zhu

“…Using scRNA-seq analysis, we identified the spermatogonia upregulated gene origin recognition complex subunit 6 (Orc6), which is involved in DNA replication and cell cycle regulation; its protein expression in the human and mouse testis was detected by western blot and immunofluorescence. …”
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A novel onco-cardiological mouse model of lung cancer-induced cardiac dysfunction and its application in identifying potential roles of tRNA-derived small RNAs by Qian Wu, Shiting Zou, Wanjie Liu, Miao Liang, Yuling Chen, Jishuo Chang, Yinghua Liu, Xiyong Yu

“…We performed sequencing to detect the tRNA-derived fragments and tRNA-derived stress-induced RNAs (tRFs/tiRNAs) expressions in mouse heart tissue. 22 upregulated and 16 downregulated tRFs/tiRNAs were identified. …”
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Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes by Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen

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Real-Time Detection of Circulating Tumor Cells in Bloodstream Using Plasmonic Fiber Sensors by Shaodi Zhu, Zhenming Xie, Yuzhi Chen, Shiyue Liu, Yiu-Wa Kwan, Shuwen Zeng, Wu Yuan, Ho-Pui Ho

“…The performance of our plasmonic fiber probe was demonstrated to specifically detect Michigan Cancer Foundation-7 (MCF-7) breast cancer cells in flowing whole mouse blood. …”
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Treatment of false‐negative metastatic lymph nodes by a lymphatic drug delivery system with 5‐fluorouracil by Honoka Fujii, Sachiko Horie, Ariunbuyan Sukhbaatar, Radhika Mishra, Maya Sakamoto, Shiro Mori, Tetsuya Kodama

“…We anticipate that the delivery of anticancer drugs by a LDDS will be of great benefit in the prevention and treatment of cancer metastasis via LNs.…”
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The Underlying Relationship between Keratoconus and Down Syndrome by Theresa Akoto, Jiemin J. Li, Amy J. Estes, Dimitrios Karamichos, Yutao Liu

“…Furthermore, this review discusses KC diagnostic and treatment strategies that may help detect KC in DS patients, as well as the available DS mouse models that could be used in modeling KC in DS patients. …”
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Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo by Jinhui Wang, Jinhui Wang, Kangxin He, Kangxin He, Zhengjiao Wu, Weikun Jin, Wende Wu, Wende Wu, Yanfeng Guo, Weiyu Zhang, Weiyu Zhang, Wenda Di, Wenda Di

“…The mouse anti-buffalo secondary antibody 2G7 conjugated to colloidal gold particles was used as the detection system for line visualization. …”
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Hepatocellular Carcinoma Detection by Cell Sensor Based on Anti-GPC3 Single-Chain Variable Fragment by Zupeng Yan, Ziyuan Che, Zhenghan Shi, Jingjiang Lv, Linhe Yang, Tianhao Lu, Yanli Lu, Jianzhen Shan, Qingjun Liu

“…The high selectivity in the liver tissue extract fluid derived from the design strategy was also displayed in the mouse samples. The customized scFv and related cell sensor demonstrated great potential for fast HCC analysis in early-stage cancer diagnosis.…”
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An ENU-induced mutation in mouse glycyl-tRNA synthetase (GARS) causes peripheral sensory and motor phenotypes creating a model of Charcot-Marie-Tooth type 2D peripheral neuropathy. by Achilli, F, Bros-Facer, V, Williams, H, Banks, G, AlQatari, M, Chia, R, Tucci, V, Groves, M, Nickols, C, Seburn, K, Kendall, R, Cader, M, Talbot, K, van Minnen, J, Burgess, R, Brandner, S, Martin, J, Koltzenburg, M, Greensmith, L, Nolan, P, Fisher, E

“…We found that enzyme activity was not reduced detectably in heterozygotes at any age, but was diminished greatly in homozygous mice compared with controls; thus, homozygous animals may suffer from a partial loss of function. …”

Applying deep learning to segmentation of murine lung tumors in pre-clinical micro-computed tomography by Mary Katherine Montgomery, Chong Duan, Lisa Manzuk, Stephanie Chang, Aiyana Cubias, Sonja Brun, Anand Giddabasappa, Ziyue Karen Jiang

“…Lung cancer remains a leading cause of cancer-related death, but scientists have made great strides in developing new treatments recently, partly owing to the use of genetically engineered mouse models (GEMMs). …”
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A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen <i>Salmonella typhimurium</i> by Palaniappan Ramasamy, Gajalakshmi Dakshinamoorthy, Shanmugam Jayashree, Dhamodharan Prabhu, Sundararaj Rajamanikandan, Palaniyandi Velusamy, Govindan Dayanithi, Robert E. B. Hanna

“…The device and technique have a range of biological applications. This novel detection system has great potential for future development and application in surveillance for microbial pathogens.…”
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Quartz Crystal Microbalance (QCM) Based Biosensor Functionalized by HER2/neu Antibody for Breast Cancer Cell Detection by Merve Yılmaz, Monireh Bakhshpour, Ilgım Göktürk, Ayşe Kevser Pişkin, Adil Denizli

“…Therefore, development of simple but effective diagnostic systems which detect the molecular markers of cancer is of great importance. …”
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In Vivo Evaluation of a Miniaturized Fluorescence Molecular Tomography (FMT) Endoscope for Breast Cancer Detection Using Targeted Nanoprobes by Hao Yang, Weipin Qian, Lily Yang, Huikai Xie, Huabei Jiang

“…This study indicates that our miniaturized FMT endoscope, coupled with the targeted nanoparticle–NIR dye conjugates as a contrast agent, has high sensitivity and specificity, and thus great potential to be used for image-guided detection and removal of a primary tumor and local metastatic tumors during surgery.…”
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