Detection of newly synthesized RNA reveals transcriptional reprogramming during ZGA and a role of Obox3 in totipotency acquisition by Mizuki Sakamoto, Aoi Ito, Sayaka Wakayama, Hiroyuki Sasaki, Teruhiko Wakayama, Takashi Ishiuchi

Get full text

Application of Six Detection Methods for Analysis of Paralytic Shellfish Toxins in Shellfish from Four Regions within Latin America by Andrew D. Turner, Sophie Tarnovius, Robert G. Hatfield, Mickael Teixeira Alves, Maggie Broadwater, Frances Van Dolah, Ernesto Garcia-Mendoza, Dinorah Medina, Maria Salhi, Alejandra B. Goya, Fernanda Barrera, Daniel Carrasco, Ignacio Rubilar, Benjamin A. Suarez-Isla

“…With the move away from use of mouse bioassay (MBA) to test bivalve mollusc shellfish for paralytic shellfish poisoning (PSP) toxins, countries around the world are having to adopt non-animal-based alternatives that fulfil ethical and legal requirements. …”
Get full text

Optical magnetic multimodality imaging of plectin-1-targeted imaging agent for the precise detection of orthotopic pancreatic ductal adenocarcinoma in mice by Wenjia Zhang, Xiaolong Liang, Liang Zhu, Xinyu Zhang, Zhengyu Jin, Yang Du, Jie Tian, Huadan Xue

“…Findings: PTP-Fe3O4-IRDye800CW nanoparticles possessed great triple-modality imaging performance and specific targeting to plectin-1 expressed on PDAC cells. …”
Get full text

A NIR ratiometric fluorescent biosensor for sensitive detection and imaging of α‐L‐fucosidase in living cells and HCC tumor‐bearing mice by Guo‐Qiang Zhang, Weiwei Feng, Zhiyuan Gao, Gao‐Lan Zhang, Xinying Wu, Yating Xiao, Xun Li, Lei Zheng, Dan Ding, Jianshuang Guo, Bo Situ

“…Abstract Detection and imaging of α‐L‐fucosidase (AFU) is of great value to understand its roles in hepatocellular carcinoma (HCC) and tumor early diagnosis, but ideal assays are still lacking. …”
Get full text

Detection of Wood Mice (<i>Apodemus sylvaticus</i>) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis by Lucía Varela-Castro, Olalla Torrontegi, Iker A. Sevilla, Marta Barral

“…Mycobacterial infections caused by the <i>Mycobacterium tuberculosis</i> complex (MTC) and non-tuberculous mycobacteria (NTM) are of great medical and veterinary relevance. The aim of this research was to study whether small mammals play a role in the epidemiology of mycobacterioses. …”
Get full text

c-Met-Targeting 19F MRI Nanoparticles with Ultralong Tumor Retention for Precisely Detecting Small or Ill-Defined Colorectal Liver Metastases by Li D, Yang J, Xu Z, Li Y, Sun Y, Wang Y, Zou H, Wang K, Yang L, Wu L, Sun X

“…Daoshuang Li,1,2,&ast; Jie Yang,1,2,&ast; Zuoyu Xu,1,2 Yingbo Li,1,2 Yige Sun,1,2 Yuchen Wang,1,2 Hongyan Zou,1,2 Kai Wang,1,2 Lili Yang,1,2 Lina Wu,1,2 Xilin Sun1,2 1Department of Nuclear Medicine, the Fourth Hospital of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China; 2NHC Key Laboratory of Molecular Probe and Targeted Diagnosis and Therapy, Molecular Imaging Research Center (MIRC) of Harbin Medical University, Harbin, Heilongjiang, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Xilin Sun; Lina Wu, Department of Nuclear Medicine, the Fourth Hospital of Harbin Medical University, 766 Xiangan N Street, Songbei District, Harbin, Heilongjiang, 150028, People’s Republic of China, Tel +86-451-88118600, Fax +86-451-82576509, Email sunxl@ems.hrbmu.edu.cn; LinaWu@hrbmu.edu.cnPurpose: Precisely detecting colorectal liver metastases (CLMs), the leading cause of colorectal cancer-associated mortality, is extremely important. 1H MRI with high soft tissue resolution plays a key role in the diagnosing liver lesions; however, precise detecting CLMs by 1H MRI is a great challenge due to the limited sensitivity. …”
Get full text

Dysregulation of miR-1-3p: An Early Event in Colitis-Associated Dysplasia by Mariana F. Fragoso, Geysson J. Fernandez, Lisa Vanderveer, Harry S. Cooper, Michael Slifker, Margie L. Clapper

“…Detection of colorectal dysplasia during surveillance colonoscopy remains the best method of determining risk for colitis-associated colorectal cancer (CAC). miRNAs (miRs) show great promise as tissue-specific biomarkers of neoplasia. …”
Get full text

Identification of functional gene modules by integrating multi-omics data and known molecular interactions by Xiaoqing Chen, Xiaoqing Chen, Mingfei Han, Yingxing Li, Xiao Li, Jiaqi Zhang, Yunping Zhu, Yunping Zhu

“…Overall, the series of evaluations demonstrated the great potential of Correlation-based Local Approximation of Membership for identifying modular biomarkers for complex diseases. …”
Get full text

MultiMap: A Tool to Automatically Extract and Analyse Spatial Microscopic Data From Large Stacks of Confocal Microscopy Images by Gherardo Varando, Ruth Benavides-Piccione, Ruth Benavides-Piccione, Alberto Muñoz, Alberto Muñoz, Alberto Muñoz, Asta Kastanauskaite, Asta Kastanauskaite, Concha Bielza, Pedro Larrañaga, Javier DeFelipe, Javier DeFelipe

“…The segmentation and quantification method has been validated over expert labeled images of the mouse hippocampus and over two benchmark datasets, obtaining comparable results to the expert detections.…”
Get full text

Significance of Second Near-Infrared Region (NIR-II) guided tumor detection and surgical treatment using Carcinoembryonic Antigen-Related Cell Adhesion Molecule (CEACAM) targeted p... by Fiza Mushtaq, Haneen Kamran

“…To compare the efficacy of NIR-11 with that of NIR-1comparative studies were also conducted on mouse CA colon, orthotopic cancer and metastasis models. …”
Get full text

Significance of Second Near-Infrared Region (NIR-II) guided tumor detection and surgical treatment using Carcinoembryonic Antigen-Related Cell Adhesion Molecule (CEACAM) targeted p... by Fiza Mushtaq, Haneen Kamran

“…To compare the efficacy of NIR-11 with that of NIR-1comparative studies were also conducted on mouse CA colon, orthotopic cancer and metastasis models. …”
Get full text

Enhanced Plasmonic Biosensor Utilizing Paired Antibody and Label-Free Fe₃O₄ Nanoparticles for Highly Sensitive and Selective Detection of Parkinson’s <i>α</i>... by Samuel Husin Surya Mandala, Tai-Jan Liu, Chiung-Mei Chen, Kuo-Kang Liu, Mochamad Januar, Ying-Feng Chang, Chao-Sung Lai, Kuo-Hsuan Chang, Kou-Chen Liu

“…<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>α</mi></semantics></math></inline-formula>-Synuclein (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>α</mi></semantics></math></inline-formula>-syn) is a potential biomarker for the early diagnosis of PD, and there is a great need to develop a biosensing platform that precisely detects <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mi>α</mi></semantics></math></inline-formula>-syn in human body fluids. …”
Get full text

Biomimetic nanobubbles for triple-negative breast cancer targeted ultrasound molecular imaging by Natacha Jugniot, Tarik F. Massoud, Jeremy J. Dahl, Ramasamy Paulmurugan

Get full text

Transendothelial electrical resistance measurement by a microfluidic device for functional study of endothelial barriers in inflammatory bowel disease by Ya Li, Min Xu, Zhu Zhu, Feng Xu, Bing Chen

“…Four electrodes independent from the outlets were integrated to the chip for TEER detection. In vivo mouse models mouse models and proteome profiling were performed to finding relevant regulators.Results: With this platform, significant decrease of TEER was detected, indicating that IBD serum exosome impact the endothelial cellular barrier on-chip. …”
Get full text

Leucocytes : methods and protocols / by Ashman, Robert B.

Emowars: Interactive Game Input Menggunakan Ekspresi Wajah by Andry Chowanda

“…The results of this research imply that happy emotion obtains 78% of detection, meanwhile the anger emotion has the lowest detection of 44.4%. …”
Get full text

Short-chain dehydrogenases/reductases (SDRs). by Kallberg, Y, Oppermann, U, Jörnvall, H, Persson, B

“…Short-chain dehydrogenases/reductases (SDRs) are enzymes of great functional diversity. Even at sequence identities of typically only 15-30%, specific sequence motifs are detectable, reflecting common folding patterns. …”

Ultrastructural description of rabies virus infection in cultured sensory neurons by Myriam L Velandia, Rosalía Pérez-Castro, Hernán Hurtado, Jaime E Castellanos

“…Primary cultures were made from adult mouse spinal ganglia for depicting an ultrastructural description of rabies virus (RABV) infection in adult mouse sensory neuron cultures; they were infected with rabies virus for 24, 36, and 48 h. …”
Get full text

Investigation of the cell biology of human regulatory T cells in the context of transplantation by Milward, K

“…After infusing labelled cells into a humanised mouse model of skin allotransplantation, we detected human Tregs within a human skin graft by PCR and visualised Tregs moving in the graft, in a live mouse, by two-photon microscopy.…”

Coenzyme-based functional assignments of short-chain dehydrogenases/reductases (SDRs). by Persson, B, Kallberg, Y, Oppermann, U, Jörnvall, H

“…Short-chain dehydrogenases/reductases (SDRs) are enzymes of great functional diversity. In spite of a residue identity of only 15-30%, the folds are conserved to a large extent, with specific sequence motifs detectable. …”