Standardization of flow cytometry and cell sorting to enable a transcriptomic analysis in a multi-site sarcoidosis study. by Roman E Magallon, Laura D Harmacek, Nicholas K Arger, Pineet Grewal, Linda Powers, Brenda R Werner, Briana Q Barkes, Li Li, Kristyn MacPhail, May Gillespie, Elizabeth K White, Sarah E Collins, Talyor Brown, Jessica Cardenas, Edward S Chen, Lisa A Maier, Sonia M Leach, Nabeel Y Hamzeh, Laura L Koth, Brian P O'Connor

“…To minimize gene expression changes induced by perturbations of T cells and avoid protein denaturation caused by freeze/thaw cycles, we optimized our protocols using freshly isolated samples at each study site. …”
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Simultaneous separation and determination of several chiral antidepressants and their enantiomers in wastewater by online heart-cutting two-dimensional liquid chromatography by Jinjian Zhong, Xin Liu, Linzhou Chen, Kan Li, Qingkun Hu, Ke Wu, Jidan Zhou, Yuesen Shi, Huajun Fan

“…The target analytes could achieve satisfactory transformation between 2D-LCs with transfer rate of 90.57–98.58 %. By means of freeze-drying and SPE, three antidepressants in wastewater were greatly preconcentrated under the optimized conditions, improving the method performance. …”
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Current Aspects for Searching and Returning Assets: Problems and Perspectives for Ukraine by P. S. Bilevskyi

“…The work on searching and returning illegally obtained assets to Ukraine is currently characterized by increased complexity and lack of efficiency due to the imperfection of existing procedures and the constant improvement of the methods to remove them from the country. The general mechanism for returning corrupt assets is based on the requirements of the criminal procedural legislation of Ukraine and provides three stages: registration of criminal proceedings; sending an official appeal to the governments of foreign states with a request to freezing the assets; sending the request to initiate criminal proceedings related to the misappropriation of assets. …”
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Pilot Study on the Molecular Pathogenesis of Pyeloureteral Junction Obstruction: Underdevelopment or Fibrosis? by Ramune Zilinskaite Tamasauske, Vytis Kazlauskas, Povilas Barasa, Natalija Krestnikova, Darius Dasevicius, Vytautas Bilius, Gilvydas Verkauskas

“…<i>Results:</i> Significant differences between healthy and stenotic tissue samples in Ctgf gene expression levels were observed, with the samples from afflicted tissue showing lower expression. …”
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Comparison of auditory cueing in toe tapping and gait in persons with Parkinson’s disease by Elizabeth L. Stegemöller, Riley Berg, Alison Warnecke, Mollie Hammer

“…Specifically, impairments in repetitive movements of the upper extremity have been associated with freezing of gait. Studies examining lower extremity repetitive movements are limited. …”
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Effect of ice formation in crevasses to the temperature field in the cold layer of glacier by G. A. Chernyakov, R. A. Chernov

“…The simulation is performed for a polythermal Arctic glacier with a regular network of crevasses filled with water at 0 °C, for the1-year period of freezing of water in crevasses in the cold layer of a glacier, below the active layer. …”
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Distribution and Degradation Processes of Isolated Permafrost near Buried Oil Pipelines by Means of Electrical Resistivity Tomography and Ground Temperature Monitoring: A Case Stud... by Gang Wu, Guoyu Li, Yapeng Cao, Dun Chen, Shunshun Qi, Fei Wang, Kai Gao, Qingsong Du, Xinbin Wang, Hongyuan Jing, Zhenrong Zhang

“…The active layer thickness has increased at a rate of 2.0 m/a from about 2.4–6.8 m to 2.5–10.8 m from 2019 to 2021 along this cross-section. …”
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Does information on age-related fertility decline and fertility policies affect university students' family and career expectations? Evidence from a randomized controlled trial. by Poh Lin Tan, Jessica Pan, Xing Xia

“…A total of 1000 undergraduate students were recruited through campus advertisements to complete a 30- to 45-minute online survey, which randomly exposed participants to one of three informational brochures on age-related fertility decline, fertility policies, or diabetes (control group). …”
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First results of Antarctic sea ice type retrieval from active and passive microwave remote sensing data by C. Melsheimer, G. Spreen, Y. Ye, M. Shokr

“…However, no data about ice type areas are available from that region, particularly for MYI. …”
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Effects of Dietary Babassu Oil or Buriti Oil on Nutrient Intake and Total Tract Digestibility, and Abomasal Digesta Fatty Acid Profile of Lambs by Nítalo Machado, Michelle Parente, Rui Bessa, Henrique Parente, Ruan Gomes, Ricardo Pinho, Daniele Ferreira, Anderson Zanine, Juliany Costa, Susana Alves

“…During the last five days of the feedlot, samples of orts and feces were individually collected to determine the nutrient and FA digestibility. …”
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Bioactive Potential of Carrot-Based Products Enriched with <i>Lactobacillus plantarum</i> by Monica Boev, Cristina Stănescu, Mihaela Turturică, Mihaela Cotârleţ, Denisa Batîr-Marin, Nicoleta Maftei, Carmen Chiţescu, Leontina Grigore-Gurgu, Vasilica Barbu, Elena Enachi, Elena Lăcrămioara Lisă

“…The content of polyphenolic compounds varied from 0.044 to 0.091 mg/g DW, while the content of total flavonoids varied from 0.03 to 0.66 mg/g DW. …”
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Major Factors Influencing the Size Distribution Analysis of Cellulose Nanocrystals Imaged in Transmission Electron Microscopy by Hui Qian

“…During TEM specimen preparation, CNCs were fractionated on TEM grids driven by the coffee-ring effect, as observed from contrast variation of CNCs with a stain-depth gradient. …”
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Zygospore development of Spirogyra (Charophyta) investigated by serial block-face scanning electron microscopy and 3D reconstructions by Sebastian J. Antreich, Sebastian J. Antreich, Charlotte Permann, Nannan Xiao, Giuseppe Tiloca, Andreas Holzinger

“…In the denser endospore, pitch angles of the microfibril layers were calculated: ~18 ± 3° in S. mirabilis, ~20 ± 3° in Spirogyra sp. from Austria and ~38 ± 8° in Spirogyra sp. from Greece. …”
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Discharge of embryos from in vitro bovine production: a sustainable vision of the process by Isabele Picada Emanuelli, Danieli Aparecida Bobbo Moreski, Ana Carolina Fanhani de Arruda Botelho, Pedro Henrique Baeza Burali, Francielli Gasparotto, Fábio Luiz Bim Cavalieri

“…On the seventh day of culture, the embryos were evaluated for viability for transfer, with some used and others discarded. …”
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Influence of Liquid Asphalt on Resilient Modules and Permanent Deformation of Recycled Asphalt Concrete by Mohammed Chaloob Saleem AL-Shujairy, Saad Isaa Sarsam

“…Two types of liquid asphalt (cutback and emulsion) were tried as recycling agents, aged materials that were reclaimed from field (100% RAP), samples were prepared from the aged mixture, and two types of liquid asphalt (cutback and emulsion) with a weight content of 0.5% were utilized to prepare a recycled mixture. …”
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Encapsulation of Jeruju Leaves Ethanol Extract (Acanthus ilicifolius L.) and It's Antiinflammatory Activities Against Carrageenan-Induced Mice by Indra Lasmana Tarigan, Tiara Kharisma, Restina Bemis, Sutrisno Sutrisno, Madyawati Latief

“…In this research, the secondary metabolite compounds contained in the ethanol extract of Jeruju Leaves were flavonoids, phenolics, steroids, and saponins. …”
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Urinary ATP may be a dynamic biomarker of detrusor overactivity in women with overactive bladder syndrome. by Miguel Silva-Ramos, Isabel Silva, Olga Oliveira, Sónia Ferreira, Maria Júlia Reis, José Carlos Oliveira, Paulo Correia-de-Sá

“…Here, we compared the urinary ATP concentration in samples collected non-invasively from OAB women with detrusor overactivity and age-matched controls. …”
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The forgotten variable? Does the euthanasia method and sample storage condition influence an organisms transcriptome – a gene expression analysis on multiple tissues in pigs by B. Chakkingal Bhaskaran, R. Meyermans, W. Gorssen, G. E. Maes, J. Buyse, S. Janssens, N. Buys

“…Further, the effect of common tissue storage conditions, RNAlater™ (RL) and snap freezing in liquid nitrogen (LN2), on gene expression profiles were also analysed. …”
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Amelioration of Motor Performance and Nigrostriatal Dopamine Cell Volume Using a Novel Far-Infrared Ceramic Blanket in an A53T Alpha-Synuclein Transgenic Parkinson’s Disease Mouse... by Frederick Robert Carrick, Luis Sebastian Alexis Valerio Hernandez, Kiminobu Sugaya

“…We had attended a Parkinson’s Disease (PD) patient for a non-healing wound who reported a marked decrease in his hand tremor and freezing of gait when his wound was exposed to a ceramic far-field infrared (cFIR) blanket. …”
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New Poly(lactic acid)–Hydrogel Core–Shell Scaffolds Highly Support MSCs’ Viability, Proliferation and Osteogenic Differentiation by Chiara Pasini, Stefano Pandini, Federica Re, Matteo Ferroni, Elisa Borsani, Domenico Russo, Luciana Sartore

“…This study introduces innovative three-dimensional bioresorbable scaffolds, in which a stiff poly(lactic acid) lattice structure is meant to ensure temporary mechanical support, while a bioactive gelatin–chitosan hydrogel is incorporated to provide a better environment for cell adhesion and proliferation. …”
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