Isolation and expansion of pure and functional γδ T cells by Tamara Verkerk, Tamara Verkerk, Tamara Verkerk, Anouk T. Pappot, Anouk T. Pappot, Tineke Jorritsma, Lisa A. King, Lisa A. King, Lisa A. King, Mariël C. Duurland, Robbert M. Spaapen, Robbert M. Spaapen, S. Marieke van Ham, S. Marieke van Ham, S. Marieke van Ham

“…Moreover, the primary focus only lies with expansion of Vδ2+ T cells, while Vδ1+ T cells likewise have anti-tumor potential. Here, we investigated whether γδ T cells directly isolated from blood could be efficiently expanded while maintaining function. γδ T cell subsets were isolated using MACS separation, followed by FACS sorting, yielding >99% pure γδ T cells. …”
Get full text

C/EBPα involvement in microglial polarization via HDAC1/STAT3 pathway aggravated sevoflurane-induced cognitive impairment in aged rats by Zhao Xu, Xi Yao, Yikang Zhao, Bo Yao

“…Methods Sprague-Dawley (SD) rats were anesthetized by inhalation of 3% sevoflurane for 6 h to establish the POCD model. …”
Get full text

Geochemical Features of Ground Ice from the Faddeevsky Peninsula Eastern Coast (Kotelny Island, East Siberian Arctic) as a Key to Understand Paleoenvironmental Conditions of Its Formation by Anfisa Pismeniuk, Petr Semenov, Alexandra Veremeeva, Wei He, Anna Kozachek, Sergei Malyshev, Elizaveta Shatrova, Anastasiia Lodochnikova, Irina Streletskaya

“…CH₄ concentrations were relatively low with a maximum value of 2.27 μmol/L. …”
Get full text

Early- and late-phase changes of brain activity and early-phase neuromodulation in the posttraumatic stress disorder rat model by Shao-Han Chang, Huan-Yuan Chen, Fu-Zen Shaw, Bai-Chuang Shyu

“…To characterize the temporal changes, we characterized freezing behavior brain wave features during the conditioning chamber re-exposure in the early (10 and 30 min; 2, 4, and 6 h) and late (day 1, 3, 7, and 14) phases after traumatic stress exposure. …”
Get full text

CONSUMER PROPERTIES OF STRUCTURE FORMING SUBSTANCES BASED ON SECONDARY RAW FISH WITH CYSTOSEIRA by Anastasiia Ivaniuta, N. Slobodianiuk, I. P. Chumachenko

“…The dynamics of dissolution of structure forming substances within 15 months were investigated to ensure the guarantee shelf life. …”
Get full text

Accelerated vegetative growth measured by gross primary productivity in China from 1980 to 2018 by Chan Zuo, Junbang Wang, Xiujuan Zhang, Alan E. Watson

“…Revealing vegetation response trends, with oscillations, is essential for formulating climate change mitigation and adaptation planning, as well as adopting ecological protection and restoration measures. Here, the long-term trend and periodic oscillations in vegetation changes were explored through a measure of gross primary production (GPP), an indicator of vegetation photosynthesis, using data of both high spatial resolution (1 km2) and a long-term of nearly 4 decades, from 1980 to 2018. …”
Get full text

A retrospective comparative study on the diagnostic efficacy and the complications: between CassiII rotational core biopsy and core needle biopsy by Shuduo Xie, Shuduo Xie, Siwei Ju, Siwei Ju, Xun Zhang, Xun Zhang, Chao Qi, Jiahang Zhang, Jiahang Zhang, Misha Mao, Misha Mao, Cong Chen, Yongxia Chen, Yongxia Chen, Feiyang Ji, Feiyang Ji, Jichun Zhou, Jichun Zhou, Linbo Wang, Linbo Wang, Linbo Wang

“…The CassiII rotational core biopsy system is a novel biopsy technique with a guide needle and a “stick-freeze” technology. The comprehensive assessments including the concordance rates of diagnosis and biomarker status between CassiII and core needle biopsy were evaluated in this study. …”
Get full text

Designing Functional Fruit-Based Recovery Drinks in Powder Form That Contain Electrolytes, Peptides, Carbohydrates and Prebiotic Fiber Taking into Account Each Component’s Osmolali... by Anna Sadowska, Franciszek Świderski, Klaudia Kulik, Bożena Waszkiewicz-Robak

“…The sensory qualities of each drink were assessed according to a scaling method. Six test versions of recovery drinks were prepared in which osmolalities ranged from 388 to 607 mOsm/kg water, total polyphenol content was 27–49 mg GAE/100 mL and vitamin C level was 8.1–20.6 mg/100 mL, following compositions defined by the study results. …”
Get full text

Clinical analysis of bronchoscope diagnosis and treatment for airway foreign body removal in pediatric patients by Lin-Lin Han, Chen Meng, Zhong-Xiao Zhang, Xiao-Di Tang, Jing Ma, Chang-Xiao Li

“…After comprehensive assessment of the foreign body nature in the airway, the foreign bodies were removed by freezing, laser, electrocoagulation, balloon and other techniques, the success rate of the foreign body removed from the airway was observed, the percentage of the foreign body removed using different techniques, the operation time, and the incidence of post-adverse reactions during operation. …”
Get full text

Low-Temperature Properties of the Sodium-Ion Electrolytes Based on EC-DEC, EC-DMC, and EC-DME Binary Solvents by Denis S. Lutsenko, Ekaterina V. Belova, Maxim V. Zakharkin, Oleg A. Drozhzhin, Evgeny V. Antipov

“…The impact is minimal in the case of EC:DEC, although it partially freezes at a higher temperature than other electrolytes. …”
Get full text

The value of serum progesterone level on day of human chorionic gonadotrophin administration / metaphase II oocyte ratio in predicting IVF/ICSI outcome in patients with normal ovar... by Ahmad Mahran, Mohammed Khairy, Reham Elkhateeb, Abdel Rahman Hegazy, Ayman Abdelmeged, Gaber El-Saber Batiha, Khalaf F. Alsharif, Helal F. Hetta, Haitham Ahmed Bahaa

“…Material & methods Two hundred patients admitted to the IVF/ICSI program at Minia IVF center in Egypt in the period from October 2016 to May 2018 were included in this study. …”
Get full text

Wildlife whodunnit: forensic identification of predators to inform wildlife management and conservation by Taylor R. Ganz, Melia T. DeVivo, Ellen M. Reese, Laura R. Prugh

“…Predator DNA was also 27.0% more likely to amplify when collected from kill sites that had not undergone a freeze‐thaw cycle between the mortality and the investigation. …”
Get full text

Use of the copepod Oithona hebes as a bioencapsulator of essential fatty acids by Marcia Vanacor Barroso, Breno Barroso Boos, Rosemar Antoniassi, Luiz Fernando Loureiro Fernandes

“…The experiment was conducted in nine cylindrical-conical tanks with a 60 L capacity, salinity of 25.8 ± 1.3, temperature of 25.5 ± 0.5 ºC and weak aeration, stocked with a density of 1.5 copepod/mL. Treatments were made in triplicate and consisted of: Treatment 1 (Control) fed with microalgae Chaetoceros gracilis and Nannochloropsis oculata (1:1) with 50,000 cells.mL-1each; Treatment 2 with S.Parkle® INVE (1g.million-1); and Treatment 3 with freeze-dried spirulina (1g.million-1). …”
Get full text

Cryopreservation of <i>Hydractinia symbiolongicarpus</i> Sperm to Support Community-Based Repository Development for Preservation of Genetic Resources by Aidan L. Huene, Jack C. Koch, Lucía Arregui, Yue Liu, Matthew L. Nicotra, Virginia M. Weis, Terrence R. Tiersch

“…The goal of this study was to establish a generalizable cryopreservation approach for <i>Hydractinia</i> that would support future repository development for other cnidarian species. Specific objectives were to: (1) characterize basic parameters related to sperm quality; (2) develop a generalizable approach for sperm collection; (3) assess the feasibility of in vitro fertilization (IVF) with sperm after refrigerated storage; (4) assess the feasibility of IVF with sperm cryopreserved with various sperm concentrations; (5) evaluate feasibility of cryopreservation with various freezing conditions, and (6) explore the feasibility of cryopreservation by use of a 3-D printed open-hardware (CryoKit) device. …”
Get full text

Assessment of Different Complementary-Relationship-Based Models for Estimating Actual Terrestrial Evapotranspiration in the Frozen Ground Regions of the Qinghai-Tibet Plateau by Chengpeng Shang, Tonghua Wu, Ning Ma, Jiemin Wang, Xiangfei Li, Xiaofan Zhu, Tianye Wang, Guojie Hu, Ren Li, Sizhong Yang, Jie Chen, Jimin Yao, Cheng Yang

“…Due to the impacts of freeze-thaw cycles and permafrost degradation on the regional ET process, it is therefore urgent and important to find a reasonable approach for ET_a estimation in the regions. …”
Get full text

La formalización lógica del lenguaje como punto de partida para el análisis objetivo del discurso y la argumentación científica by William Orlando Cárdenas-Marín, Darwin Bellini Reyes Solís, Frank Bolívar Viteri Bazante

“…¿Cuáles son las limitaciones que presenta la lógica clásica o aristotélica ante la lógica simbólica estructurada por Frege? ¿Cuáles son los aportes a partir de la formalización lógica del lenguaje para el análisis del discurso y la estructuración de la ciencia?…”
Get full text

Experimental study of a 3D-printing technique combined with biphasic calcium phosphates to treat osteonecrosis of the femoral head in a canine model by Zhian Chen, Fanzhe Feng, Xixiong Su, Yongqing Xu, Ying Zhang, Hongbo Tan

“…Methods Fifteen healthy adult male and female beagle dogs weighing 20 + 2 kg were randomly divided into three groups (n = 5) after establishing a model of ONFH using the liquid nitrogen freezing method. …”
Get full text

APPLICATION OF MATHEMATICAL METHODS OF THE ANALYSIS IN THE SELECTION OF THE VEGETABLE PEA by S. P. Kuzmina, N. G. Kazydub, E. V. Bondarenko

“…As a result of the research, sources of selected economically valuable pea vegetable seeds were selected for breeding in the conditions of the southern forest-steppe of Western Siberia: Chinese, Bondi, Chika and Italy for shrinking vegetation period, for resistance to ascochitis and rust - Chinese, Chika, Gloriosa, for weight gain One bean – Chinese, Chika and Italy; Weight of beans and seeds from a single plant – Chinese, Chika, Italy, Bondi and Demos, 1000 seeds of weight – Chinese, Chika and Italy, according to the taste and suitability for canning and freezing - Chinese and Chika, by the number of nitrogen fixing nodules on one plant - Chinese. …”
Get full text

Investigation on Performances and Functions of Asphalt Mixtures Modified with Super Absorbent Polymer (SAP) by Yuxuan Sun, Weimin Song, Hao Wu, Yiqun Zhan, Zhezheng Wu, Jian Yin

“…The water stability and infiltration reduction effect of the asphalt mixtures incorporated with SAP were evaluated by the Marshall stability test, immersion Marshall stability test, freeze-thaw splitting strength test, Cantabro test, and permeability test. …”
Get full text

Impact of ice formations on the shore and bottom areas of shallow seas and large lakes of middle and subarctic latitudes by S. V. Maznev, S. A. Ogorodov

“…Lawrence, the Baltic, Black, Caspian Seas, and Sea of Japan, freeze partially or completely every year. Inland and northern lakes and seas of the Subarctic, such as the White Sea, the Gulf of Bothnia and the Sea of Okhotsk are characterized by the most severe ice conditions. …”
Get full text