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    Structure and functions of a metallo-beta-lactamase- like hypothetical protein bleg1_2437 from alkali-tolerant soil bacterium Bacillus lehensis G1 by Tan, Soo Huei

    Published 2015
    “…This recombinant plasmid was transformed into Escherichia coli Rosetta-gami (DE3) for Bleg1_2437 protein expression and purification. The optimum condition for intracellular expression of Bleg1_2437 was achieved at 20 ºC, with Isopropyl-β-D-Thiogalactopyranoside (IPTG) concentration of 0.1 mM and incubated for 18 hours. …”
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