A Comprehensive Review on l-Asparaginase and Its Applications
l-asparaginase (LA) catalyzes the degradation of asparagine, an essential amino acid for leukemic cells, into ammonia and aspartate. Owing to its ability to inhibit protein biosynthesis in lymphoblasts, LA is used to treat acute lymphoblastic leukemia (ALL). Different isozymes of this enzyme have be...
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| Format: | Article |
| Language: | English |
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Springer
2016
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| Online Access: | http://umpir.ump.edu.my/id/eprint/12747/1/A%20Comprehensive%20Review%20on%20l-Asparaginase%20and%20Its%20Applications.pdf |
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| author | Batool, Tahira Makky, Essam A. Ali, Muna Jalal M. M., Yusoff |
| author_facet | Batool, Tahira Makky, Essam A. Ali, Muna Jalal M. M., Yusoff |
| author_sort | Batool, Tahira |
| collection | UMP |
| description | l-asparaginase (LA) catalyzes the degradation of asparagine, an essential amino acid for leukemic cells, into ammonia and aspartate. Owing to its ability to inhibit protein biosynthesis in lymphoblasts, LA is used to treat acute lymphoblastic leukemia (ALL). Different isozymes of this enzyme have been isolated from a wide range of organisms, including plants and terrestrial and marine microorganisms. Pieces of information about the three-dimensional structure of l-asparaginase from Escherichia coli and Erwinia sp. have identified residues that are essential for catalytic activity. This review catalogues the major sources of l-asparaginase, the methods of its production through the solid state (SSF) and submerged (SmF) fermentation, purification, and characterization as well as its biological roles. In the same breath, this article explores both the past and present applications of this important enzyme and discusses its future prospects. |
| first_indexed | 2024-03-06T12:02:48Z |
| format | Article |
| id | UMPir12747 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:02:48Z |
| publishDate | 2016 |
| publisher | Springer |
| record_format | dspace |
| spelling | UMPir127472018-07-26T06:50:56Z http://umpir.ump.edu.my/id/eprint/12747/ A Comprehensive Review on l-Asparaginase and Its Applications Batool, Tahira Makky, Essam A. Ali, Muna Jalal M. M., Yusoff Q Science (General) l-asparaginase (LA) catalyzes the degradation of asparagine, an essential amino acid for leukemic cells, into ammonia and aspartate. Owing to its ability to inhibit protein biosynthesis in lymphoblasts, LA is used to treat acute lymphoblastic leukemia (ALL). Different isozymes of this enzyme have been isolated from a wide range of organisms, including plants and terrestrial and marine microorganisms. Pieces of information about the three-dimensional structure of l-asparaginase from Escherichia coli and Erwinia sp. have identified residues that are essential for catalytic activity. This review catalogues the major sources of l-asparaginase, the methods of its production through the solid state (SSF) and submerged (SmF) fermentation, purification, and characterization as well as its biological roles. In the same breath, this article explores both the past and present applications of this important enzyme and discusses its future prospects. Springer 2016 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/12747/1/A%20Comprehensive%20Review%20on%20l-Asparaginase%20and%20Its%20Applications.pdf Batool, Tahira and Makky, Essam A. and Ali, Muna Jalal and M. M., Yusoff (2016) A Comprehensive Review on l-Asparaginase and Its Applications. Applied Biochemistry and Biotechnology, 178 (5). pp. 900-923. ISSN 1559-0291. (Published) http://dx.doi.org/10.1007/s12010-015-1917-3 DOI: 10.1007/s12010-015-1917-3 |
| spellingShingle | Q Science (General) Batool, Tahira Makky, Essam A. Ali, Muna Jalal M. M., Yusoff A Comprehensive Review on l-Asparaginase and Its Applications |
| title | A Comprehensive Review on l-Asparaginase and Its Applications |
| title_full | A Comprehensive Review on l-Asparaginase and Its Applications |
| title_fullStr | A Comprehensive Review on l-Asparaginase and Its Applications |
| title_full_unstemmed | A Comprehensive Review on l-Asparaginase and Its Applications |
| title_short | A Comprehensive Review on l-Asparaginase and Its Applications |
| title_sort | comprehensive review on l asparaginase and its applications |
| topic | Q Science (General) |
| url | http://umpir.ump.edu.my/id/eprint/12747/1/A%20Comprehensive%20Review%20on%20l-Asparaginase%20and%20Its%20Applications.pdf |
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