Loop protein engineering for improved transglycosylation activity of a β‐N Acetylhexosaminidase

Certain enzymes of the glycoside hydrolase family 20 (GH20) exert transglycosylation activity and catalyze the transfer of β‐N‐acetylglucosamine (GlcNAc) from a chitobiose donor to lactose to produce lacto‐N‐triose II (LNT2), a key human milk oligosaccharide backbone moiety. The present work is aime...

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Bibliographic Details
Main Authors: Shariza, Jamek, Muschiol, Jan, Holck, Jesper, Zeuner, Birgitte, Busk, Peter K., Mikkelsen, Jørn D., Meyer, Anne S.
Format: Article
Language:English
Published: John Wiley & Sons 2018
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/22565/1/Loop%20protein%20engineering%20for%20improved%20transglycosylation%20activity.pdf