Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques
The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids,...
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| Format: | Article |
| Language: | English |
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Taylor & Francis
2019
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| Online Access: | http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf |
| _version_ | 1825812661755969536 |
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| author | Alara, Oluwaseun Ruth Nour, A. H. Ishmael, Ukaegbu Chinonso Kabbashi, Nassereldeen Ahmed |
| author_facet | Alara, Oluwaseun Ruth Nour, A. H. Ishmael, Ukaegbu Chinonso Kabbashi, Nassereldeen Ahmed |
| author_sort | Alara, Oluwaseun Ruth |
| collection | UMP |
| description | The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique. |
| first_indexed | 2024-03-06T12:32:28Z |
| format | Article |
| id | UMPir24723 |
| institution | Universiti Malaysia Pahang |
| language | English |
| last_indexed | 2024-03-06T12:32:28Z |
| publishDate | 2019 |
| publisher | Taylor & Francis |
| record_format | dspace |
| spelling | UMPir247232020-02-21T07:34:21Z http://umpir.ump.edu.my/id/eprint/24723/ Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques Alara, Oluwaseun Ruth Nour, A. H. Ishmael, Ukaegbu Chinonso Kabbashi, Nassereldeen Ahmed QD Chemistry QK Botany TP Chemical technology The bioactive compounds in Vernonia amygdalina leaf were extracted using ethanol through Soxhlet and microwave-assisted extraction (MAE) techniques. The phytochemical analysis was carried out on the ethanolic extracts using the standard techniques. This analysis revealed the presence of flavonoids, alkaloids, steroids, terpenoids, glycosides, tannins, phenols, saponins, and the absence of anthraquinones. Furthermore, gas chromatography-mass spectroscopy (GC-MS) analysis results revealed the presence of more phytochemicals in the extract obtained through MAE compared to Soxhlet extraction technique, whereby phytol was present in a higher percentage. Fourier transform infrared spectroscopy analysis confirmed the presence of phytochemicals in the extracts. Moreover, the IC50 values of the extracts antioxidant activities were evaluated using DPPH and ABTS assays. There exists a clear correlation between total phenolic content, total flavonoid content and antioxidant activity of the extracts. Therefore, this study suggested that higher phenolic compounds responsible for natural antioxidant could be obtained from V. amygdalina leaf using the microwave-assisted extraction technique. Taylor & Francis 2019-12-11 Article PeerReviewed pdf en cc_by_nc_4 http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Ishmael, Ukaegbu Chinonso and Kabbashi, Nassereldeen Ahmed (2019) Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques. Journal of Taibah University for Science, 13 (1). pp. 414-422. ISSN 1658-3655. (Published) https://doi.org/10.1080/16583655.2019.1582460 https://doi.org/10.1080/16583655.2019.1582460 |
| spellingShingle | QD Chemistry QK Botany TP Chemical technology Alara, Oluwaseun Ruth Nour, A. H. Ishmael, Ukaegbu Chinonso Kabbashi, Nassereldeen Ahmed Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title | Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title_full | Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title_fullStr | Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title_full_unstemmed | Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title_short | Extraction and characterization of bioactive compounds in Vernonia amygdalina leaf ethanolic extract comparing Soxhlet and microwave-assisted extraction techniques |
| title_sort | extraction and characterization of bioactive compounds in vernonia amygdalina leaf ethanolic extract comparing soxhlet and microwave assisted extraction techniques |
| topic | QD Chemistry QK Botany TP Chemical technology |
| url | http://umpir.ump.edu.my/id/eprint/24723/1/Extraction%20and%20characterization%20of%20bioactive%20compounds%20in%20Vernonia.pdf |
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