The use of melting curves as a novel approach for validation of real-time PCR instruments
Validation of PCR thermal cycler performance is crucial in order to obtain reliable results. In this study, high resolu- tion melting curve (HRM) analysis is presented as a novel validation method for real-time PCR instruments. By ap- plying HRM analysis using a defined PCR amplicon and EvaGreen dye...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2011-09-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000113735 |