Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice

Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice

Summary: Chimeric antigen receptor (CAR) T cell therapy often causes serious toxicities, such as cytokine release syndrome (CRS), mainly due to interleukin-6 (IL-6) secreted by monocyte lineage cells. Here, we describe a protocol to generate anti-CD19 CAR T cells and quantify human monocyte-derived...

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Bibliographic Details
Main Authors: Thao Nguyen, Toshiaki Yoshikawa, Yusuke Ito, Hitomi Kasuya, Takahiro Nakashima, Sachiko Okamoto, Yasunori Amaishi, Haosong Zhang, Yang Li, Tetsuya Matsukawa, Satoshi Inoue, Yuki Kagoya
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Cell culture
Flow Cytometry
Cancer
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005884

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http://www.sciencedirect.com/science/article/pii/S2666166724005884

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