A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

Abstract Background The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of p...

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Bibliographic Details
Main Authors: Charlotte Bureau, Nadège Lanau, Mathieu Ingouff, Boukhaddaoui Hassan, Anne-Cécile Meunier, Fanchon Divol, Rosie Sevilla, Delphine Mieulet, Anne Dievart, Christophe Périn
Format: Article
Language:English
Published: BMC 2018-10-01
Series:Plant Methods
Subjects:
Rice
Root meristem
Multiphoton microscope
GFP
YFP
CFP
Online Access:http://link.springer.com/article/10.1186/s13007-018-0364-x

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http://link.springer.com/article/10.1186/s13007-018-0364-x

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