Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats
IntroductionRates of relapse to drug use during abstinence are among the highest for opioid use disorder (OUD). In preclinical studies, reinstatement to drug-seeking has been extensively studied as a model of relapse–but the work has been primarily in males. We asked whether biological sex contribut...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.1035350/full |
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| author | Suman K. Guha Yanaira Alonso-Caraballo Gillian S. Driscoll Jessica A. Babb Megan Neal Nicholas J. Constantino Tania Lintz Elizabeth Kinard Elena H. Chartoff |
| author_facet | Suman K. Guha Yanaira Alonso-Caraballo Gillian S. Driscoll Jessica A. Babb Megan Neal Nicholas J. Constantino Tania Lintz Elizabeth Kinard Elena H. Chartoff |
| author_sort | Suman K. Guha |
| collection | DOAJ |
| description | IntroductionRates of relapse to drug use during abstinence are among the highest for opioid use disorder (OUD). In preclinical studies, reinstatement to drug-seeking has been extensively studied as a model of relapse–but the work has been primarily in males. We asked whether biological sex contributes to behaviors comprising self-administration of the prescription opioid oxycodone in rats, and we calculated the relative contribution of these behavioral measures to reinstatement in male and female rats.Materials and methodsRats were trained to self-administer oxycodone (8 days, training phase), after which we examined oxycodone self-administration behaviors for an additional 14 days under three conditions in male and female rats: short access (ShA, 1 h/d), long access (LgA, 6 h/d), and saline self-administration. All rats were then tested for cue-induced reinstatement of drug-seeking after a 14-d forced abstinence period. We quantified the # of infusions, front-loading of drug intake, non-reinforced lever pressing, inter-infusion intervals, escalation of intake, and reinstatement responding on the active lever.ResultsBoth male and female rats in LgA and ShA conditions escalated oxycodone intake to a similar extent. However, males had higher levels of non-reinforced responding than females under LgA conditions, and females had greater levels of reinstatement responding than males. We then correlated each addiction-related measure listed above with reinstatement responding in males and females and ranked their respective relative contributions. Although the majority of behavioral measures associated with oxycodone self-administration did not show sex differences on their own, when analyzed together using partial least squares regression, their relative contributions to reinstatement were sex-dependent. Front-loading behavior was calculated to have the highest relative contribution to reinstatement in both sexes, with long and short inter-infusion intervals having the second greatest contribution in females and males, respectively.DiscussionOur results demonstrate sex differences in some oxycodone self-administration measures. More importantly, we demonstrate that a sex- dependent constellation of self-administration behaviors can predict the magnitude of reinstatement, which holds great promise for relapse prevention in people. |
| first_indexed | 2024-04-12T06:44:22Z |
| format | Article |
| id | doaj.art-005683e86c464e29a143be413e11b5a2 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-12T06:44:22Z |
| publishDate | 2022-11-01 |
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| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-005683e86c464e29a143be413e11b5a22022-12-22T03:43:35ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532022-11-011610.3389/fnbeh.2022.10353501035350Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female ratsSuman K. Guha0Yanaira Alonso-Caraballo1Gillian S. Driscoll2Jessica A. Babb3Megan Neal4Nicholas J. Constantino5Tania Lintz6Elizabeth Kinard7Elena H. Chartoff8Basic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesResearch Service, VA Boston Healthcare System, Department of Psychiatry, Harvard Medical School, Boston, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesBasic Neuroscience Division, McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, United StatesIntroductionRates of relapse to drug use during abstinence are among the highest for opioid use disorder (OUD). In preclinical studies, reinstatement to drug-seeking has been extensively studied as a model of relapse–but the work has been primarily in males. We asked whether biological sex contributes to behaviors comprising self-administration of the prescription opioid oxycodone in rats, and we calculated the relative contribution of these behavioral measures to reinstatement in male and female rats.Materials and methodsRats were trained to self-administer oxycodone (8 days, training phase), after which we examined oxycodone self-administration behaviors for an additional 14 days under three conditions in male and female rats: short access (ShA, 1 h/d), long access (LgA, 6 h/d), and saline self-administration. All rats were then tested for cue-induced reinstatement of drug-seeking after a 14-d forced abstinence period. We quantified the # of infusions, front-loading of drug intake, non-reinforced lever pressing, inter-infusion intervals, escalation of intake, and reinstatement responding on the active lever.ResultsBoth male and female rats in LgA and ShA conditions escalated oxycodone intake to a similar extent. However, males had higher levels of non-reinforced responding than females under LgA conditions, and females had greater levels of reinstatement responding than males. We then correlated each addiction-related measure listed above with reinstatement responding in males and females and ranked their respective relative contributions. Although the majority of behavioral measures associated with oxycodone self-administration did not show sex differences on their own, when analyzed together using partial least squares regression, their relative contributions to reinstatement were sex-dependent. Front-loading behavior was calculated to have the highest relative contribution to reinstatement in both sexes, with long and short inter-infusion intervals having the second greatest contribution in females and males, respectively.DiscussionOur results demonstrate sex differences in some oxycodone self-administration measures. More importantly, we demonstrate that a sex- dependent constellation of self-administration behaviors can predict the magnitude of reinstatement, which holds great promise for relapse prevention in people.https://www.frontiersin.org/articles/10.3389/fnbeh.2022.1035350/fullescalationfront-loadingfemalevulnerabilityopioidrelapse |
| spellingShingle | Suman K. Guha Yanaira Alonso-Caraballo Gillian S. Driscoll Jessica A. Babb Megan Neal Nicholas J. Constantino Tania Lintz Elizabeth Kinard Elena H. Chartoff Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats Frontiers in Behavioral Neuroscience escalation front-loading female vulnerability opioid relapse |
| title | Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats |
| title_full | Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats |
| title_fullStr | Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats |
| title_full_unstemmed | Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats |
| title_short | Ranking the contribution of behavioral measures comprising oxycodone self-administration to reinstatement of drug-seeking in male and female rats |
| title_sort | ranking the contribution of behavioral measures comprising oxycodone self administration to reinstatement of drug seeking in male and female rats |
| topic | escalation front-loading female vulnerability opioid relapse |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2022.1035350/full |
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